New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi 2230679 =57 (1.030)4 (0.080) 4/266 7.6 6.9% coding (56/399 nt) yohJ UPF0299 family inner membrane protein
?NC_000913_3_pae_pgi 2230701 = 55 (1.060)coding (78/399 nt) yohJ UPF0299 family inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATT  >  NC_000913_3_pae_pgi/2230534‑2230827
                                                                                                                                                 |                                                                                                                                                    
gAAAAAATAAGCGCTGATTTCTTAATGTGATCGATAGCACGTTTTACGAATTAATTGTATAATGAATTCATCTCATCTGGGGAGTTGATTATTAGTGAGCCACTGAACATTATGTGGCAGTATTTACGACTTTTCGGCCAGAGTTAtcc                                                                                                                                                   >  2:320603/1‑147 (MQ=255)
    aaaTAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTAtgcctgc                                                                                                                                               <  1:120192/149‑1 (MQ=255)
    aaaTAAGCGCTGATTTCTTAATGTGATCGGGAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTAtgcctgc                                                                                                                                               <  1:278627/149‑1 (MQ=255)
          gcgcTGATTTCTGAATGTGATCGGTAGCACGTTTTACGAATTAATTGTCTGGCAGGGCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTAt                                                                                                                                         <  1:273146/149‑1 (MQ=255)
             cTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTAGGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCa                                                                                                                                      <  1:79288/149‑1 (MQ=255)
              tgttttCTTAATGTGATCGGTGGCACGTTTTACGCATTAATTGTATGATGAATCCATCTCATCTGGGGTGGTGATTATGAGTAAGACACTGAATATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAg                                                                                                                                     <  2:441495/146‑1 (MQ=255)
              tGATTTCTTAATGTGATCGGTAGCACTTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAg                                                                                                                                     <  1:25731/149‑1 (MQ=255)
                 tttCTTAATGGGATAGGTAGCACGTTTTAGGGATTAATTGTGTGATGACTTCATCGCATCTGGGGTGTTGATTTTGAGTAAGACACTGAACATTATTTGGCAATATTTACGCTCTTCAGTCCTATTTTATGCCTACCTGTATGCAGGCa                                                                                                                                  >  2:99585/1‑149 (MQ=255)
                  ttCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCAt                                                                                                                                 >  1:392109/1‑149 (MQ=255)
                       aaTGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCa                                                                                                                            <  2:22652/149‑1 (MQ=255)
                       aaTGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCGCACCTTGGGTGGTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCa                                                                                                                            >  2:495314/1‑149 (MQ=255)
                         tgtgGTCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCAACTGGTGAAATTGGGATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCAtt                                                                                                                          <  1:225829/149‑1 (MQ=255)
                               cGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTcc                                                                                                                    <  2:596736/149‑1 (MQ=255)
                               cGGTAGCACGTGTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTCTATCATGAGTAAGACACTCAACATTATCTGGCAAGATTCACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTcc                                                                                                                    <  1:437582/149‑1 (MQ=255)
                                 gTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCt                                                                                                                  <  2:266832/149‑1 (MQ=255)
                                    gCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCt                                                                                                               >  1:29252/1‑149 (MQ=255)
                                      acattttACAAATTAATAGTAAGATGAATCAATCTCATCTGGGGCGTTGATTATCAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTATTCCTGAATTATGCTTGCCTTTATGCAGGCATTTTCATTGCTTCCCCTCTAc                                                                                                             >  2:422215/4‑149 (MQ=255)
                                        gTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCg                                                                                                           <  2:370116/149‑1 (MQ=255)
                                          ttggcGAATTAATTGTATGGTGTAGCCATTTCATTTGGGGTGTTGATTATGAGTAAGTCACTTGACATTATCTCGCAATATTTGCGCGCTTTCGTCCTGATTTATGCCTTCCTGTATGCAGGCATGTTCATTGCTTCCCTGCTACCGGt                                                                                                         <  2:419582/145‑1 (MQ=255)
                                             aCGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAc                                                                                                      <  1:727620/149‑1 (MQ=255)
                                               gAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCTTTGCTTCTCTGGTATCGGTTACCa                                                                                                    >  1:886117/1‑149 (MQ=255)
                                                aattaattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCAt                                                                                                   >  1:182062/1‑149 (MQ=255)
                                                aattaattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCAt                                                                                                   >  1:780129/1‑149 (MQ=255)
                                                   taattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATCAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTcc                                                                                                <  2:2241/149‑1 (MQ=255)
                                                      ttGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAGCCATTCAggg                                                                                             >  1:610686/1‑149 (MQ=255)
                                                              tgaATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGcatca                                                                                     >  1:541615/1‑149 (MQ=255)
                                                                   ccATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTTATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCggg                                                                                <  1:134304/149‑1 (MQ=255)
                                                                   ccATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCggg                                                                                <  2:29252/149‑1 (MQ=255)
                                                                    catctcatctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGa                                                                               >  2:642319/1‑149 (MQ=255)
                                                                          atctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGa                                                                         >  1:300950/1‑149 (MQ=255)
                                                                          atctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGa                                                                         >  1:301137/1‑149 (MQ=255)
                                                                            ctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATc                                                                       <  2:16118/149‑1 (MQ=255)
                                                                                ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGt                                                                   <  1:966636/149‑1 (MQ=255)
                                                                                  tgtTGATTATGAGAAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTGCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGttt                                                                 <  2:558792/149‑1 (MQ=255)
                                                                                   gtTGATTATGAGTAAGACACTGAACATTAGCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTg                                                                <  1:547850/149‑1 (MQ=255)
                                                                                    ttGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGt                                                               >  2:815312/1‑149 (MQ=255)
                                                                                            gAGTAAGACACTGAACATTATCTGGCAAAATTTACGCTGGTTCTTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTgg                                                       <  1:179697/149‑1 (MQ=255)
                                                                                              gTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGcc                                                     >  1:940345/1‑149 (MQ=255)
                                                                                              gTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGcc                                                     <  1:642319/149‑1 (MQ=255)
                                                                                                 aGACACTGAACATTATCTGGCAATATTTATGCGCTTTCGTCCTGATTTATGCATGCCTGTATGCAGGCATTTTCATTGCTTCCCTCCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTCTTTCTCCTGCTGGCCTTg                                                  >  2:910836/1‑149 (MQ=255)
                                                                                                     acTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTTTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTCACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGTTGGCCTTGCaaa                                              >  1:337686/1‑149 (MQ=255)
                                                                                                       tGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAtt                                            >  2:125420/1‑149 (MQ=255)
                                                                                                        gAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAtt                                            >  1:561453/1‑148 (MQ=255)
                                                                                                        gAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAtt                                            <  2:561453/148‑1 (MQ=255)
                                                                                                            aTTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTcc                                       >  1:314843/1‑149 (MQ=255)
                                                                                                                 cTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGAAAATTCTTCCGGcca                                  >  1:247428/1‑147 (MQ=255)
                                                                                                                  tGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCaaa                                 >  2:466827/1‑149 (MQ=255)
                                                                                                                     cAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATg                              <  2:863386/149‑1 (MQ=255)
                                                                                                                       atatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATggg                            >  2:394016/1‑149 (MQ=255)
                                                                                                                         atTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTc                          >  1:910761/1‑149 (MQ=255)
                                                                                                                               gcgcTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTTTTTGTCCTGCTGGCCTTGCAGATTCTTCCGGCAAAATGGGTCaaacag                    >  1:266617/1‑145 (MQ=255)
                                                                                                                                      cGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCTTTTTCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCt             <  1:2498/149‑1 (MQ=255)
                                                                                                                                        tCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCACTCCGGGGTGCtcc           >  2:663152/1‑147 (MQ=255)
                                                                                                                                           tGATTTATGCCTGCCTTTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTa        >  2:604889/1‑149 (MQ=255)
                                                                                                                                           tGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTa        >  2:369308/1‑149 (MQ=255)
                                                                                                                                            gATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAATAAAACCCTTCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTAc       <  1:950246/149‑1 (MQ=255)
                                                                                                                                            gATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAGCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTGCGTAc       >  1:643842/1‑149 (MQ=255)
                                                                                                                                                 aTGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGAtt  <  2:910761/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATT  >  NC_000913_3_pae_pgi/2230534‑2230827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.