Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_pgi 1299499–1300696 1300697 2–1199 46 [0] [0] 46 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 12994980 (0.000)45 (1.150) 44/272 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCA  >  NC_000913_3_pae_pgi/1300553‑1300842
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tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                               <  1:653097‑M2/4‑1 (MQ=255)
  aaattacagttagttataggtatgtccttaactgcttctcctcaccatcatgttattttcgcctcatcataatcctgggcttgctgaagaataattgaattgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                             <  2:650929‑M2/6‑1 (MQ=255)
     tgacatttagttataagtatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggtttgctgaagaataatggaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                          <  2:102584‑M2/9‑1 (MQ=255)
            tagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                   <  1:238688‑M2/16‑1 (MQ=255)
               ttataaggatttccttaactgcttctcctaaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccacttccttttcagatggaaagtgccaaATAATAATCAATACTtaat                                                                                                                                >  2:164082‑M2/131‑148 (MQ=255)
                    aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                           >  2:2474‑M2/126‑149 (MQ=255)
                     ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgcggaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaAAAATAATCAATTGTTaaatgataa                                                                                                                          >  2:421906‑M2/125‑147 (MQ=255)
                           ccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                  >  1:598268‑M2/119‑119 (MQ=255)
                           ccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                  <  2:598268‑M2/1‑1 (MQ=255)
                             ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTc                                                                                                                  >  1:102586‑M2/117‑149 (MQ=255)
                              taactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaacgtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                                 <  1:533636‑M2/34‑1 (MQ=255)
                               aactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                                >  1:655901‑M2/115‑149 (MQ=255)
                                 ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTCTTGTGCATTTCacta                                                                                                              >  1:433459‑M2/113‑149 (MQ=255)
                                  tgcttctcctcaccatcatgttattttcgcacgttatcgatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactac                                                                                                             >  1:534172‑M2/112‑149 (MQ=255)
                                     ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                          >  1:327839‑M2/109‑149 (MQ=255)
                                        tcctcgccatcatgttattgtcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                       <  1:55032‑M2/44‑1 (MQ=255)
                                         cctcaaccttatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagatgaaagtgctcaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                      <  2:355705‑M2/45‑1 (MQ=255)
                                           tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                            <  1:453602‑M2/7‑1 (MQ=255)
                                           tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                            >  2:453602‑M2/103‑109 (MQ=255)
                                             accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                           >  2:608186‑M2/101‑140 (MQ=255)
                                             accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                           <  1:608186‑M2/40‑1 (MQ=255)
                                               catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaaggatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTAACTACTGGAACAGTAATc                                                                                                >  1:677515‑M2/99‑149 (MQ=255)
                                                atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattacttccgctgcctttggtagaggaaagtgctaaATAATAATCAATCATTAAATTATTGTGAATTTTACTACTggacctgacatcc                                                                                               >  2:285485‑M2/98‑143 (MQ=255)
                                                      ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                         >  1:412158‑M2/92‑149 (MQ=255)
                                                          tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                     >  1:154649‑M2/88‑149 (MQ=255)
                                                           ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                    <  1:144101‑M2/63‑1 (MQ=255)
                                                                tacttcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                               <  2:165002‑M2/68‑1 (MQ=255)
                                                                cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAAAAACGATAAACATGc                                                                               >  2:112080‑M2/82‑149 (MQ=255)
                                                                        actcctgggcttgctgaagaataattgaaatgatattattacttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTGCATTATTGTGCATTTTACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCaa                                                                       <  1:285485‑M2/76‑1 (MQ=255)
                                                                            ctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAgctc                                                                   >  1:567901‑M2/70‑149 (MQ=255)
                                                                                gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATTCTTAGCCAATCTCTAtt                                                               >  1:425977‑M2/66‑149 (MQ=255)
                                                                                     ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTACCCAATctctattggctt                                                          >  1:2905‑M2/61‑144 (MQ=255)
                                                                                           aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                    >  1:17827‑M2/55‑149 (MQ=255)
                                                                                             taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaattgaa                                                  <  2:17827‑M2/97‑1 (MQ=255)
                                                                                                     atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                          <  1:112080‑M2/105‑1 (MQ=255)
                                                                                                      tgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAGTTGAAAGAtgtttg                                         >  2:576302‑M2/44‑149 (MQ=255)
                                                                                                          attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                        <  2:416484‑M2/107‑1 (MQ=255)
                                                                                                          attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                        >  1:416484‑M2/40‑146 (MQ=255)
                                                                                                                     cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                          >  2:57315‑M2/29‑101 (MQ=255)
                                                                                                                     cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                          <  1:57315‑M2/73‑1 (MQ=255)
                                                                                                                        tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                       <  1:502945‑M2/124‑1 (MQ=255)
                                                                                                                                      gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                            <  1:613443‑M2/71‑1 (MQ=255)
                                                                                                                                      gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                            >  2:613443‑M2/12‑82 (MQ=255)
                                                                                                                                      gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAt         >  2:72138‑M2/12‑149 (MQ=255)
                                                                                                                                           tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt    <  2:327839‑M2/143‑1 (MQ=255)
                                                                                                                                             ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa  <  2:154649‑M2/145‑1 (MQ=255)
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GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCA  >  NC_000913_3_pae_pgi/1300553‑1300842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: