New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_pgi	2955129 =	15 (1.000)	3 (0.210)	3/278	1.8	18.5%	coding (875/3543 nt)	recB	exonuclease V (RecBCD complex), beta subunit
	?	NC_000913_3_vch_pgi	2955151 =	12 (0.830)					coding (853/3543 nt)	recB	exonuclease V (RecBCD complex), beta subunit
Rejected: Frequency below/above cutoff threshold.

TCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACC  >  NC_000913_3_vch_pgi/2955003‑2955283                                                                                                                                                     |                                                                                                                                     ttaGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGa                                                                                                                                      <  2:266409/147‑1 (MQ=255)                           aTCGCGGATCGATAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtct                                                                                                            <  2:158845/149‑1 (MQ=255)                                ggATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCTTGCGATCTTCTAAGAAACGCTGGTAGAATTTTTCCACCGACTCCGCCAACTGATAACTGTTTGTCtcttct                                                                                                       >  1:214015/1‑149 (MQ=255)                                                 gCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGAt                                                                                      <  1:220403/149‑1 (MQ=255)                                                  cAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATc                                                                                     >  1:213286/1‑149 (MQ=255)                                                       aGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAATGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTc                                                                                <  2:229725/149‑1 (MQ=255)                                                           gctcgatcgCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATc                                                                            <  1:17001/147‑1 (MQ=255)                                                               gatcgCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTACGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGGTAACGGTTTGTCTCTTCTTCTGCCCAGGGGCTGATCTTGTCGATCCAtt                                                                        >  2:5776/1‑149 (MQ=255)                                                                     atcgacaagatcagcgcctgggcagaagaagagacaaacagttatcagttgccggagtCGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtctc                                                                                                           <  2:66390‑M1/50‑1 (MQ=255)                                                                     atcgacaagatcagcgcctgggcagaagaagagacaaacagttatcagttgccggagtCGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtctc                                                                                                           >  1:66390‑M1/59‑108 (MQ=255)                                                                             gTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGACACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTa                                                          <  2:171405/149‑1 (MQ=255)                                                                                     cgcgGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCCACGGTTaaa                                                  <  2:203712/149‑1 (MQ=255)                                                                                                   gagacaaacagttatcagttgccggagtcgctggaaaaattctcccagcgACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGAt                                                                             <  2:66390‑M2/58‑1 (MQ=255)                                                                                                   gagacaaacagttatcagttgccggagtcgctggaaaaattctcccagcgACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGAt                                                                             >  1:66390‑M2/51‑108 (MQ=255)                                                                                                                cGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTc                       <  1:49953/149‑1 (MQ=255)                                                                                                                 gATCTTCTAAGAAACGCTGGGAGAATTTTTCCGGCGACTCCGGCAACTGATATCTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATGCATTTAGCCTGATTGCTAAGGTTAGACTTGCGTCGATCAATACCGGGAGATTCg                      >  2:145033/1‑149 (MQ=255)                                                                                                                                     gcgcattctgccagtgACGTCGGCAACTTCTAACTGTTTGTCTGTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAATATTCGATCAGCGCATCCAGTTCAcc  <  1:255071‑M2/133‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                     TCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACC  >  NC_000913_3_vch_pgi/2955003‑2955283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.