New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_pgi	2505963 =	27 (1.400)	3 (0.160)	3/278	2.5	11.1%	coding (670/1086 nt)	ypdF	Xaa‑Pro aminopeptidase
	?	NC_000913_3_vch_pgi	2505985 =	22 (1.190)					coding (648/1086 nt)	ypdF	Xaa‑Pro aminopeptidase
Rejected: Frequency below/above cutoff threshold.

CGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACA  >  NC_000913_3_vch_pgi/2505816‑2506081                                                                                                                                                    |                                                                                                                       gagtggtttatgcgccagcagggcgcagaaaaagcctcttttgacaccattgtcgccagtggctggcgtggggcgctgccgcacggcaaagccagcgacaagattgttgcagcgggcgagtttgtcactctcgatttcggtgcgctgTa                                                                                                                       <  2:171566‑M1/2‑1 (MQ=255)      tCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCAAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCgga                                                                                                                  >  1:321461/1‑149 (MQ=255)         tCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCa                                                                                                               >  1:168930/1‑149 (MQ=255)               tgctgGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGcc                                                                                                         >  2:282876/1‑149 (MQ=255)               tgctgGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGcc                                                                                                         >  2:282836/1‑149 (MQ=255)                 ctgGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTCAACCACAGGCGATTAATGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTTATATCAGAGCAGTAGCCCt                                                                                                       <  2:242641/149‑1 (MQ=255)                     gggcacagaaaaagcctcttttgacaccattgtcgccagtggctggcgtggggcgctgccgcacggcaaagccagcgacaagattgttgcagcgggcgagtttgtcactctcgatttcgttgcgctgTATCAGAGCAGTAGCCCTGATa                                                                                                   >  1:288093‑M1/128‑149 (MQ=255)                      aGCGCACGCCGGGGCGAATCGCGGAGGTTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTGAACGGCAGGTGAGATTGGGGGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCGGAGCAGTAGCCCTGATAc                                                                                                  >  2:259438/1‑149 (MQ=255)                                        tCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGAAAGGTGCGCGTCATATCAGAGCAGTCGCCCTGCTACAGCGCACCGAAATCgaga                                                                                >  1:147328/1‑149 (MQ=255)                                            ggAGATGGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGa                                                                            <  2:55754/149‑1 (MQ=255)                                                   gctgctGGCTTTGCCTGCCGGACAATTTGATCGGCGTTAATCAGCCGGTTACATTCGGCGCTCAGCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTc                                                                     <  2:273911/149‑1 (MQ=255)                                                     tgcGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAg                                                                                                >  2:63585/1‑120 (MQ=255)                                                     tgcGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAg                                                                                                <  1:63585/120‑1 (MQ=255)                                                     tgcGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGc                                                                   >  2:265373/1‑149 (MQ=255)                                                            tgtgCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGAAGTAGCCCTGATACAGCCCACCGAAATCGAGAGTGACAAACTCCCCCGCTGc                                                            >  2:149474/1‑149 (MQ=255)                                                                  tGCAGGACGATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAAt                                                      <  1:118773/149‑1 (MQ=255)                                                                   gCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATc                                                     <  1:282876/149‑1 (MQ=255)                                                                   gCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATc                                                     <  1:282836/149‑1 (MQ=255)                                                                      ggACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGTGGGAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTg                                                  >  1:334445/1‑149 (MQ=255)                                                                           aTTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACCCGCCCGCTGCAACAGTCTTGTCCCt                                             >  2:79748/1‑149 (MQ=255)                                                                            tttGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCaga                                                                                                                  <  2:200607/79‑1 (MQ=255)                                                                            tttGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCaga                                                                                                                  >  1:200607/1‑79 (MQ=255)                                                                                       ttAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTg                                                             <  1:298626/121‑1 (MQ=255)                                                                                       ttAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTg                                                             >  2:298626/1‑121 (MQ=255)                                                                                             agcagGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAg                           >  1:221172/1‑149 (MQ=255)                                                                                                    gagaTTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCa                    <  1:268561/149‑1 (MQ=255)                                                                                                               agcgggcgagtttgtcactctcgatttcggtgcgctgTATCAGAGCAGTAGCCCTGATACa                                                                                                 <  1:99120‑M1/24‑1 (MQ=255)                                                                                                               agcgggcgagtttgtcactctcgatttcggtgcgctgTATCAGAGCAGTAGCCCTGATACa                                                                                                 >  2:99120‑M1/38‑61 (MQ=255)                                                                                                                tCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTg        >  1:315/1‑149 (MQ=255)                                                                                                                     ccTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCCGATACAGCGCACCGCGAACCAGACTGACAAACTCGCCCGCTGCAACAATCTTTTCACTAGCTTTGCCCTACGGCAGCGCCCCACGCCAGCCACTGGCGAc   >  2:165961/1‑149 (MQ=255)                                                                                                                      cTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCCCAGAATTCCAGGGTGACAAACTCGCCCGATGCAAAAATCTTACCGCAGGCTTTGCCGTGCGCCCGCTCCCCGCGCCCGCCCCTGGCcaca  >  2:237076/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                       CGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACA  >  NC_000913_3_vch_pgi/2505816‑2506081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.