New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_pgi	213093 =	24 (1.310)	4 (0.230)	4/276	2.0	14.8%	coding (763/1299 nt)	tilS	tRNA(Ile)‑lysidine synthetase
	?	NC_000913_3_vch_pgi	213111 =	23 (1.310)					coding (781/1299 nt)	tilS	tRNA(Ile)‑lysidine synthetase
Rejected: Frequency below/above cutoff threshold.

CGAAGCAACGGCCCGCAGCGCCGCACTTTGTGCTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCC  >  NC_000913_3_vch_pgi/212948‑213231                                                                                                                                                  |                                                                                                                                           accacagttgcgactgatagcgtcggatttcaaacgcgcccaaacgtaaacagggtgaggcatcttcccccgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctaccctgccagccagcgg                                                                                                                                         <  2:316494‑M1/4‑1 (MQ=255)      cAACGGCCCGCAGCGCCGCACTTTGTGCTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGAtt                                                                                                                                    <  1:33605/149‑1 (MQ=255)                  tagcgtcggacttcaaacgcgcccaaacgtaaacagggtgaggcatcttcccgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgccagccaGCGGCGATTCTCCGCCgctgg                                                                                                                        >  1:203469‑M1/129‑149 (MQ=255)                   gcgtcggatttcaaacgcgcctaaacgtaaacagggtgaggcatcttcccgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgcccgccgcGCGGCGATTATCCGCCgctggc                                                                                                                       >  2:324084‑M1/128‑149 (MQ=255)                           tttGGGCTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTggcaggg                                                                                                               <  2:20954/149‑1 (MQ=255)                                 cTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAAt                                                                                                         >  2:90659/1‑149 (MQ=255)                                 cTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAAt                                                                                                         >  1:39687/1‑149 (MQ=255)                                    aaCACGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGCGATTCCCGCCGCGCGGCGATTATCCGCCGCTGGCTCGCAGGGCAGAATGCa                                                                                                      <  1:173534/149‑1 (MQ=255)                                    aaCAAGAGAGCCTGCTGGATAAACTGCTGGCAGAACATTCAGCAAACAGTCAAACGCCGGAAGGGGCACTCCGGATTGGGCCAATGCTGGGGATGCGTGGTGCCAGCCTCGCGAAGATTACCCGACGCTCGATTGGAGGACAGAATGCa                                                                                                      >  2:187152/1‑149 (MQ=255)                                       aaGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATGGCCGCAGGGGACGCTGAAGATTGTGCCAATGCTGGCGATGAGGGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATACAcca                                                                                                   >  1:103213/1‑148 (MQ=255)                                       aaGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCACTACACCAGATCGGGGGGGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCg                                                                                                   >  1:156200/1‑149 (MQ=255)                                              ccTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGCCGCTGCAGCATGTGCCAATGCTGTCGATAAGTGGTTCTGGCTGGTCGGCGAGCAGCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCtt                                                                                            >  2:311202/1‑149 (MQ=255)                                                  ctgGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATTCTCCGCTGGCTGTCAGGGCAGAATGCACCGATGCCTTCCcg                                                                                        >  1:59576/1‑149 (MQ=255)                                                    ggATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCAGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCcgcg                                                                                      >  1:211120/1‑149 (MQ=255)                                                              ctgGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCCCGACGCGTTGGt                                                                            >  1:235046/1‑149 (MQ=255)                                                                 gCAGATGATTTCGCACACTGTCAATCGCCGCAGGGGCCGCTGCAGATTGTGCTAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAg                                                                         <  2:257910/149‑1 (MQ=255)                                                                    gatgatTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGAt                                                                      <  2:146432/149‑1 (MQ=255)                                                                     cgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgccagccaGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCa                                                                                                      >  2:41172‑M1/78‑116 (MQ=255)                                                                     cgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgccagccaGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCa                                                                                                      <  1:41172‑M1/39‑1 (MQ=255)                                                                      tgatGTAGCACCCGGGCAAGCGCCGCAGGGGATGCTGCAGCCTGTGCCAATGCGGGCGATGAGGGATGCCGGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGGATGCAACGATGCCTTGGCGCGACGCGTTGGTGTGGAttt                                                                    <  2:6101/149‑3 (MQ=255)                                                                       gatTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTg                                                                   >  1:8192/1‑149 (MQ=255)                                                                             gCACATTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAggg                                                             >  2:281039/1‑148 (MQ=255)                                                                                  cTGTCAATCGCCGGAGGGGACGGTGAAGTTTGTGCAAATGCTGGCGATGAGAGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtgg                                                        <  1:14316/149‑1 (MQ=255)                                                                                       aaTCGCCGCAGGGGACGCTGCAGATTGTGCGAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcgctg                                                   <  1:254019/149‑1 (MQ=255)                                                                                           gccgcAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGACGGCACCGATGCCTTCCCGCGACGCGTTCGTGAGGATCTGGCAGGAAGtggcgctggcgc                                               >  2:288078/1‑149 (MQ=255)                                                                                                       gCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCt                                   >  1:299781/1‑149 (MQ=255)                                                                                                               ttGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGt                           <  1:207955/149‑1 (MQ=255)                                                                                                                                     tGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGaaa     >  1:291514/1‑149 (MQ=255)                                                                                                                                        tGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATcc  >  1:145861/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                           CGAAGCAACGGCCCGCAGCGCCGCACTTTGTGCTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCC  >  NC_000913_3_vch_pgi/212948‑213231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.