New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi 213093 =24 (1.310)4 (0.230) 4/276 2.0 14.8% coding (763/1299 nt) tilS tRNA(Ile)‑lysidine synthetase
?NC_000913_3_vch_pgi 213111 = 23 (1.310)coding (781/1299 nt) tilS tRNA(Ile)‑lysidine synthetase
Rejected: Frequency below/above cutoff threshold.

GCGCCGCACTTTGTGCTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGT  >  NC_000913_3_vch_pgi/212965‑213255
                                                                                                                                                  |                                                                                                                                                
tagcgtcggacttcaaacgcgcccaaacgtaaacagggtgaggcatcttcccgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgccagccaGCGGCGATTCTCCGCCgctgg                                                                                                                                                >  1:203469‑M1/129‑149 (MQ=255)
 gcgtcggatttcaaacgcgcctaaacgtaaacagggtgaggcatcttcccgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgcccgccgcGCGGCGATTATCCGCCgctggc                                                                                                                                               >  2:324084‑M1/128‑149 (MQ=255)
         tttGGGCTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTggcaggg                                                                                                                                       <  2:20954/149‑1 (MQ=255)
               cTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAAt                                                                                                                                 >  2:90659/1‑149 (MQ=255)
               cTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAAt                                                                                                                                 >  1:39687/1‑149 (MQ=255)
                  aaCACGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGCGATTCCCGCCGCGCGGCGATTATCCGCCGCTGGCTCGCAGGGCAGAATGCa                                                                                                                              <  1:173534/149‑1 (MQ=255)
                  aaCAAGAGAGCCTGCTGGATAAACTGCTGGCAGAACATTCAGCAAACAGTCAAACGCCGGAAGGGGCACTCCGGATTGGGCCAATGCTGGGGATGCGTGGTGCCAGCCTCGCGAAGATTACCCGACGCTCGATTGGAGGACAGAATGCa                                                                                                                              >  2:187152/1‑149 (MQ=255)
                     aaGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATGGCCGCAGGGGACGCTGAAGATTGTGCCAATGCTGGCGATGAGGGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATACAcca                                                                                                                           >  1:103213/1‑148 (MQ=255)
                     aaGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCACTACACCAGATCGGGGGGGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCg                                                                                                                           >  1:156200/1‑149 (MQ=255)
                            ccTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGCCGCTGCAGCATGTGCCAATGCTGTCGATAAGTGGTTCTGGCTGGTCGGCGAGCAGCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCtt                                                                                                                    >  2:311202/1‑149 (MQ=255)
                                ctgGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATTCTCCGCTGGCTGTCAGGGCAGAATGCACCGATGCCTTCCcg                                                                                                                >  1:59576/1‑149 (MQ=255)
                                  ggATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCAGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCcgcg                                                                                                              >  1:211120/1‑149 (MQ=255)
                                            ctgGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCCCGACGCGTTGGt                                                                                                    >  1:235046/1‑149 (MQ=255)
                                               gCAGATGATTTCGCACACTGTCAATCGCCGCAGGGGCCGCTGCAGATTGTGCTAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAg                                                                                                 <  2:257910/149‑1 (MQ=255)
                                                  gatgatTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGAt                                                                                              <  2:146432/149‑1 (MQ=255)
                                                   cgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgccagccaGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCa                                                                                                                              >  2:41172‑M1/78‑116 (MQ=255)
                                                   cgcgccagcgccacttcctgccagatcctcaccaacgcgtcgcgggaaggcatcggtgcattctgccctgccagccaGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCa                                                                                                                              <  1:41172‑M1/39‑1 (MQ=255)
                                                    tgatGTAGCACCCGGGCAAGCGCCGCAGGGGATGCTGCAGCCTGTGCCAATGCGGGCGATGAGGGATGCCGGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGGATGCAACGATGCCTTGGCGCGACGCGTTGGTGTGGAttt                                                                                            <  2:6101/149‑3 (MQ=255)
                                                     gatTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTg                                                                                           >  1:8192/1‑149 (MQ=255)
                                                           gCACATTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAggg                                                                                     >  2:281039/1‑148 (MQ=255)
                                                                cTGTCAATCGCCGGAGGGGACGGTGAAGTTTGTGCAAATGCTGGCGATGAGAGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtgg                                                                                <  1:14316/149‑1 (MQ=255)
                                                                     aaTCGCCGCAGGGGACGCTGCAGATTGTGCGAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcgctg                                                                           <  1:254019/149‑1 (MQ=255)
                                                                         gccgcAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGACGGCACCGATGCCTTCCCGCGACGCGTTCGTGAGGATCTGGCAGGAAGtggcgctggcgc                                                                       >  2:288078/1‑149 (MQ=255)
                                                                                     gCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCt                                                           >  1:299781/1‑149 (MQ=255)
                                                                                             ttGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGt                                                   <  1:207955/149‑1 (MQ=255)
                                                                                                           tgcattctgccctgccagccagcggcggataatcgccgcTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGcgcg                                                                      <  1:41172‑M2/77‑1 (MQ=255)
                                                                                                           tgcattctgccctgccagccagcggcggataatcgccgcTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGcgcg                                                                      >  2:41172‑M2/40‑116 (MQ=255)
                                                                                                                   tGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGaaa                             >  1:291514/1‑149 (MQ=255)
                                                                                                                      tGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATcc                          >  1:145861/1‑149 (MQ=255)
                                                                                                                           gccagcggcggataatcgccgcgCGGCGGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTAGGCGCGTTTGAAATCCGACGc                     >  2:324084‑M2/24‑149 (MQ=255)
                                                                                                                             ccagcggcggagaatcgccgcTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAGTCCGACGCTa                   >  1:203469‑M2/22‑149 (MQ=255)
                                                                                                                                              ccgcTGGCTGGCAGGGTAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGGGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTgtggt  <  2:316494‑M2/145‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCGCCGCACTTTGTGCTGAACAAGAGAGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGT  >  NC_000913_3_vch_pgi/212965‑213255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.