New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi 3645087 =17 (0.930)4 (0.230) 4/274 1.9 19.9% coding (96/2043 nt) prlC oligopeptidase A
?NC_000913_3_vch_pgi 3645120 = 16 (0.920)coding (63/2043 nt) prlC oligopeptidase A
Rejected: Frequency below/above cutoff threshold.

GGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGC  >  NC_000913_3_vch_pgi/3644945‑3645215
                                                                                                                                              |                                                                                                                                
ggCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCTGCTGGCAGAGCTTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTc                                                                                                                            >  1:288033/1‑149 (MQ=255)
ggCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTc                                                                                                                            >  2:210843/1‑149 (MQ=255)
  cTATTTTGCACCGATGTCCGGGGGCTGATCGGGGAGAAGATGCGACCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCaa                                                                                                                          <  2:103499/149‑1 (MQ=255)
     tctttCACCGAGTTCAGGTGGCCGACCGGGGAGAAGATTCTTCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGc                                                                                                                       <  2:285532/147‑1 (MQ=255)
           aCCGAGTTCAGGTGGCTGACCGGGGAGACGATACGCCCCAACACATCGTCAACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCACTACTACGCGCCCCACCTTTTCGCGGCAATCGTTCCATCCCCTatc                                                                                                                 >  2:104176/1‑147 (MQ=255)
             cGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCa                                                                                                               <  1:210843/149‑1 (MQ=255)
               aGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCCATGCCTTAGTCACg                                                                                                             >  1:179431/1‑149 (MQ=255)
                 ttCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGc                                                                                                           >  1:214727/1‑149 (MQ=255)
                               cGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATggt                                                                                             >  2:188822/1‑147 (MQ=255)
                                      aagaTACACCCCAACACATCGTCCACTACCGCCAGCCGCCGGCACAGATAGTCCCAGGTGTACGGTGCCCCATACGCTACGACGCGCTCCACAATCTCCCTGCAGCCCGTCAACGCCTTAGCCCCGGCTGAACCGACAAGTCCCggaaa                                                                                      >  2:101805/1‑146 (MQ=255)
                                                      caTCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGa                                                                      <  2:55415/149‑1 (MQ=255)
                                                         ctctttaaccaggactgcgcgaatgacgaatccgttactgactccctttgaattgcctccgttttctaaaattctcccggaacatGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGga                                                                                       >  1:21726‑M1/86‑129 (MQ=255)
                                                         ctctttaaccaggactgcgcgaatgacgaatccgttactgactccctttgaattgcctccgttttctaaaattctcccggaacatGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGga                                                                                       <  2:21726‑M1/44‑1 (MQ=255)
                                                                                           ccAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTCTAAAAAACGGAGGCAAATCAAAGGGAGTCAGTAACGGCTTCGTCATTc                                 >  1:43444/1‑149 (MQ=255)
                                                                                                      gTGCCCCTTGCGCTAGTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACTGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTg                      <  2:258154/149‑1 (MQ=255)
                                                                                                                cgcTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAgg            >  2:146155/1‑149 (MQ=255)
                                                                                                                 gcTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGt           >  1:306730/1‑149 (MQ=255)
                                                                                                                          cgcTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAAGTTAAGGAAgc  <  1:15261/149‑1 (MQ=255)
                                                                                                                          cgcTCCACGTTTGCGCGGCAGTCGTTCAATCCCTTAGTCACGGCTGGAACGATATGTGCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACTGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAgc  <  1:14365/149‑1 (MQ=255)
                                                                                                                                             tGTCGTTCAATGCCTTAGTCACGGCTGGAACGACa                                                                                                 >  1:48128‑M1/2‑35 (MQ=255)
                                                                                                                                             tGTCGTTCAATGCCTTAGTCACGGCTGGAACGACa                                                                                                 <  2:48128‑M1/34‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                
GGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTCAGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGC  >  NC_000913_3_vch_pgi/3644945‑3645215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.