Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913_3_vch_pgi	2,393,422	Δ13 bp	coding (1613‑1625/1842 nt)	nuoL ←	NADH:ubiquinone oxidoreductase, membrane subunit L

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_pgi	= 2393421	1 (0.050)	15 (0.690)	15/288	0.5	93.8%	coding (1626/1842 nt)	nuoL	NADH:ubiquinone oxidoreductase, membrane subunit L
	?	NC_000913_3_vch_pgi	2393435 =	1 (0.050)					coding (1612/1842 nt)	nuoL	NADH:ubiquinone oxidoreductase, membrane subunit L

AGCAGACCTTTACCTGCAAAGCGGGAAAGGACAGCCGGGATGTTCATCATTGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTCACCAGAGTACGTTTACCCAGCCACAGCCAGGCTGCCAGCAGAATGCCGACCACCGCGACCACGCCAGAGGTAATT  >  NC_000913_3_vch_pgi/2393296‑2393565                                                                                                                              |                                                                                                                                                 agcagACCTTTACCTGCAAAGCGGGAAAGGACAGCCGGGATGTTCATCATGGAGTTAAGCGGAGCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACGTAGCCATCAAGCCAGcgttgtaccaccacgcccccagc                                                                                                                           >  2:323576‑M1/1‑126 (MQ=255)         tttACCTGCAAAGCGGGAAAGGACAGCCGGGATGTTCATCATTGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGcgttgtaccaccaggtgcccagccgacggcc                                                                                                                   <  1:81696‑M1/149‑32 (MQ=255)                     gctggaaaGGACAGCCGGGATGTTCATCAGTGAGTTCAGCGGTTCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGcgttgtaccaccaggtgcccagcagacggcccggcgcactgtt                                                                                                       <  2:147327‑M1/146‑44 (MQ=255)                       gggAAAGGACAGCCGGGATGTTCATCATTGAGTTCAGCGGATCGCGTTTCAGCAACCATGCAATACCCAGGAAGGGCGTGACGAACACTGTGTCATACACCCAGcgttgtaccaccaggtgcccagcaggcggcccggcgcactgttgg                                                                                                     <  2:259427‑M1/149‑46 (MQ=255)                            aGGAAAGCCGGGATGTTCATCATTGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGcgttgtaccaccaggtgcccagcagacggcccggcgcactgttggcgatg                                                                                                <  2:178118‑M1/149‑51 (MQ=255)                              gACAGCCGGGATGTTCATCATTGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTGTGTCATACAGCCCGcgttgtaccaccaggtgcccagcagacggcccggcgcactgttggcgatgga                                                                                              <  1:256020‑M1/149‑53 (MQ=255)                                           ttCATCATTGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGCACGGCTTGACGAACACTTTGTCATACAGCCAGcgttgtaccaccaggtgcccagcagacggcccggcgcactgttggcgatggaggtcaccagagta                                                                                 >  1:251951‑M1/1‑84 (MQ=255)                                                  gtgAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGcgttgtaccaccaggtgcccagcagacggcccggcgcactgttggcgatggaggtcaccagagtacgtttac                                                                          <  1:346107‑M1/148‑73 (MQ=255)                                                                     tttCAGCAACCGGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGcgttgtaccaccaggtgcccagcagacggcccggcggactgttggcgatggaggtcaccagagtacgtttacccagccacagccaggctgc                                                       <  2:43791‑M1/149‑92 (MQ=255)                                                                     tttCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACCCTTTGTCATACAGCCAGcgttggcccaccaggtgcccagcagacggcccggcgcactgttggcgctggaggtcaccagagcacgattccgcagccacccccacgctcc                                                       >  2:380132‑M1/1‑58 (MQ=255)                                                                      ttCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAG‑‑‑‑‑‑‑‑‑‑‑CCC‑‑GCGTTGTACCACCAGGTGCCCAGCAGACCGCCCAGCGAACTGTAGGCGATGGAGAGCACCCGAAGACGCTCACCCAGCCACACCCCGgccgcc                                         >  2:158718/1‑149 (MQ=255)                                                                                         ccAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGcgttgtaccaccaggtgcacagcagacggcccggagcactgttggcgatggaggtcaccagagtacgtttacccagccacagccaggctgccagcagaatgccgaccaccg                                   >  1:85008‑M1/1‑38 (MQ=255)                                                                                                    ttGGCGAACACTTTGTCATACAGCCTGcgttgtaccaccaggtgcccagcagacggcccggcgcactgttggcgatggaggtcaccagagtacgtttacccggccacagccaggctgtcagcggtatgccgaccaccgcgaccacgcca                        <  2:361205‑M1/149‑123 (MQ=255)                                                                                                      gaagaacACTCTGTCATACAGCCAGcgttgtaccaccaggtgcccagcagacggcccggcgcactattggcgatggaggtcaccagaggacgtttacccagccacagccacgctgccagcagaatgccgaccaccgcgaccacgccaga                      <  2:18602‑M1/146‑125 (MQ=255)                                                                                                                   tCATACAGCCAGcgttttaccaccaggtgcccagcagacggcccggcgcactgttggcgatggaggtcaccagagtacgtttacccagccacagccagtctgccaacagaatgccgaccaccgcgaccacgccagaggtaatttccagg         <  2:164341‑M1/149‑138 (MQ=255)                                                                                                                          gccagcgttgtaccaccaggtgcccagcagacggcccggcgcactgttggcgatggaggtcaccagagtacggttacccagccacagccaggctgccagcagaatgccgaccaccgcgaccacgccggcggtaatttccagggtcaaca  >  2:300393‑M1/1‑5 (MQ=255)                                                                                                                              |                                                                                                                                                 AGCAGACCTTTACCTGCAAAGCGGGAAAGGACAGCCGGGATGTTCATCATTGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTCACCAGAGTACGTTTACCCAGCCACAGCCAGGCTGCCAGCAGAATGCCGACCACCGCGACCACGCCAGAGGTAATT  >  NC_000913_3_vch_pgi/2393296‑2393565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.