Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1299499–1300697	1300697	1–1199	49 [0]	[0] 48	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	47 (1.140)	40/278	0.1	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299350‑1299646                                                                                                                                                     |                                                                                                                                                     cttctCAAATTACCGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCCTCATGGTATTTTCGCCACATCATAATCCTGGGCTTACTGAAGAATAATTGAAATGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAA                                                                                                                                                      >  2:609319‑M1/1‑149 (MQ=255)     tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                                  >  1:76508‑M1/1‑145 (MQ=255)       aagttacaCTTAGTTATAAGGCTTGCCTTAACTGCTTCTCCTCACCAGCATGTTATTTTCGCAACATCATAATCCCGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                                <  2:76246‑M1/146‑7 (MQ=255)            aCAGTTAGTTATAAGGATTTCCTTAATTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                           <  1:326701‑M1/149‑12 (MQ=255)            aCAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                           <  2:574830‑M1/149‑12 (MQ=255)            aCAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                           <  2:489739‑M1/149‑12 (MQ=255)                  agttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATAAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                                                                                                                     <  1:526557‑M1/149‑18 (MQ=255)                   gttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                                    >  1:599716‑M1/1‑131 (MQ=255)                                  ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                                                                                                                     >  1:607813‑M1/1‑116 (MQ=255)                                     aCTGCTTCTCCTCACTAACATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact                                                                                                                  <  2:127372‑M1/149‑37 (MQ=255)                                       tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                                >  1:507583‑M1/1‑111 (MQ=255)                                            ctcctcACGATCATGTTATTTTCGCCACCTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                           <  1:288686‑M1/149‑44 (MQ=255)                                               ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcgtttcgatactggaactg                                                                                                        >  1:285184‑M1/1‑103 (MQ=255)                                                   ccATCATGTTATTTTCTCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                    <  1:406648‑M1/149‑51 (MQ=255)                                                    catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactattggaactgtaatg                                                                                                   >  1:87661‑M1/1‑98 (MQ=255)                                                           ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                            <  1:316964‑M1/149‑59 (MQ=255)                                                           ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                            >  1:488991‑M1/1‑91 (MQ=255)                                                              ttttCGCCACATCATAATCCTGGGCTTGCTGAAGACTATTGGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                         >  1:181025‑M1/1‑88 (MQ=255)                                                              ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATATCAGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                         >  1:180962‑M1/1‑88 (MQ=255)                                                              ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                         <  1:463154‑M1/149‑62 (MQ=255)                                                                         tcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                              <  1:317588‑M1/149‑73 (MQ=255)                                                                            tAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaatatagacatgcttagcca                                                                           >  1:334601‑M1/1‑74 (MQ=255)                                                                             aaTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttcaattattgtgcatttcactaatggaactgtaataagaaaagatagacaagattagccaa                                                                          >  1:723303‑M1/1‑73 (MQ=255)                                                                                         gCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGATTGTGTTGGaatataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                              <  1:743631‑M1/149‑89 (MQ=255)                                                                                              agaaTAATTGAAAGGTTATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaagtattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat                                                         <  1:482670‑M1/149‑94 (MQ=255)                                                                                                aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatcactattagattgaattg                                                       >  1:60210‑M1/1‑54 (MQ=255)                                                                                                   aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                    >  2:243539‑M1/1‑51 (MQ=255)                                                                                                      tgtaatgaTATTATTAATCTCCTTGCTTTTGGTAGAGGAAAGTGGTAAataataatcaattgttaaattattgtgcatgtcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                                 <  2:299980‑M1/146‑102 (MQ=255)                                                                                                      tcaaatgTTCTTATTAATTCCAATGTCCTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttcgccaaactctatttgattggattcaaagtt                                                 <  2:508261‑M1/147‑102 (MQ=255)                                                                                                      tGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattcaactactgtaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                                 <  1:143419‑M1/149‑102 (MQ=255)                                                                                                        atatgATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTCAataataatcaattgttaaattatggtgcatttcacttctggatctgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgt                                               <  2:148573‑M1/147‑104 (MQ=255)                                                                                                        aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGGTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgt                                               <  2:148618‑M1/149‑104 (MQ=255)                                                                                                           tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAatagtaatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttg                                            >  1:682882‑M1/1‑43 (MQ=255)                                                                                                            gttattattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                           <  1:468271‑M1/147‑108 (MQ=255)                                                                                                                  attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaccactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttg                                            <  1:578791‑M1/142‑107 (MQ=255)                                                                                                                  attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaccactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttg                                            >  2:578791‑M1/1‑36 (MQ=255)                                                                                                                        tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                               <  1:319692‑M1/149‑120 (MQ=255)                                                                                                                        tCCACTCCCTTTGGTAGAGGAAAGGTCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                               <  2:285184‑M1/149‑120 (MQ=255)                                                                                                                          cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                          >  2:256432‑M1/1‑28 (MQ=255)                                                                                                                          cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                          <  1:256432‑M1/120‑93 (MQ=255)                                                                                                                           aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                            <  2:599716‑M1/149‑123 (MQ=255)                                                                                                                              gCCTTTGGTAGAGGAAAGTGCTAAataataatcactttttaaagtattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                         <  1:396990‑M1/149‑126 (MQ=255)                                                                                                                                 tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcacgctat                      >  1:441075‑M1/1‑21 (MQ=255)                                                                                                                                 tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                      <  1:289577‑M1/149‑129 (MQ=255)                                                                                                                                     gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatacttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat                  >  2:569199‑M1/1‑17 (MQ=255)                                                                                                                                          ggAAAGTGCTAAataataatcaattgttacattattgtgcatttcactactggaactgtaatcagaaaagatagccatgcttagccaatctctatttgatttaattgaaagatgtttgttaaggcatggatgccagctatcgattcggt             >  2:369001‑M1/1‑12 (MQ=255)                                                                                                                                           gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagtttctgat            >  1:505970‑M1/1‑11 (MQ=255)                                                                                                                                            aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata           <  1:243539‑M1/149‑140 (MQ=255)                                                                                                                                                     aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaata  >  2:547748‑M1/1‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299350‑1299646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑