Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1299499–1300697	1300697	1–1199	49 [0]	[0] 48	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	47 (1.140)	40/278	0.1	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_hsa_tpiA/1300553‑1300845                                                                                                                                                  |                                                                                                                                                    tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                                  >  1:76508‑M2/146‑149 (MQ=255)   aagttacacttagttataaggcttgccttaactgcttctcctcaccagcatgttattttcgcaacatcataatcccgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                                <  2:76246‑M2/6‑1 (MQ=255)        acagttagttataaggatttccttaattgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                           <  1:326701‑M2/11‑1 (MQ=255)        acagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                           <  2:574830‑M2/11‑1 (MQ=255)        acagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                           <  2:489739‑M2/11‑1 (MQ=255)              agttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattataaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTa                                                                                                                                     <  1:526557‑M2/17‑1 (MQ=255)               gttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                                    >  1:599716‑M2/132‑149 (MQ=255)                              ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTc                                                                                                                     >  1:607813‑M2/117‑149 (MQ=255)                                 actgcttctcctcactaacatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCact                                                                                                                  <  2:127372‑M2/36‑1 (MQ=255)                                   tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactac                                                                                                                >  1:507583‑M2/112‑149 (MQ=255)                                        ctcctcacgatcatgttattttcgccacctcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                           <  1:288686‑M2/43‑1 (MQ=255)                                           ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCGTTTCGATACTGGAACTg                                                                                                        >  1:285184‑M2/104‑149 (MQ=255)                                               ccatcatgttattttctccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                    <  1:406648‑M2/50‑1 (MQ=255)                                                catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTATTGGAACTGTAatg                                                                                                   >  1:87661‑M2/99‑148 (MQ=255)                                                       ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                            <  1:316964‑M2/58‑1 (MQ=255)                                                       ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                            >  1:488991‑M2/92‑149 (MQ=255)                                                          ttttcgccacatcataatcctgggcttgctgaagactattggaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                         >  1:181025‑M2/89‑149 (MQ=255)                                                          ttttcgccacatcataatcctgggcttgctgaagaatatcagaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                         >  1:180962‑M2/89‑149 (MQ=255)                                                          ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                         <  1:463154‑M2/61‑1 (MQ=255)                                                                     tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                              <  1:317588‑M2/72‑1 (MQ=255)                                                                        taatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAATATAGACATGCTTAGCCa                                                                           >  1:334601‑M2/75‑149 (MQ=255)                                                                         aatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTCAATTATTGTGCATTTCACTAATGGAACTGTAATAAGAAAAGATAGACAAGATTAGCCaa                                                                          >  1:723303‑M2/74‑149 (MQ=255)                                                                                     gctgaagaataattgaaatgatattattaattccactgcctttggtagaggattgtgttggAATATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                              <  1:743631‑M2/88‑1 (MQ=255)                                                                                          agaataattgaaaggttattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAAGTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                         <  1:482670‑M2/93‑1 (MQ=255)                                                                                            aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCACTATTAGattgaattg                                                       >  1:60210‑M2/55‑149 (MQ=255)                                                                                               aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                    >  2:243539‑M2/52‑149 (MQ=255)                                                                                                  tgtaatgatattattaatctccttgcttttggtagaggaaagtggtaaATAATAATCAATTGTTAAATTATTGTGCATGTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                                 <  2:299980‑M2/101‑1 (MQ=255)                                                                                                  tgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTCAACTACTGTAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                                 <  1:143419‑M2/101‑1 (MQ=255)                                                                                                  tcaaatgttcttattaattccaatgtccttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTCGCCAAACTCTATTTGATTGGATTCAaagtt                                                 <  2:508261‑M2/101‑3 (MQ=255)                                                                                                    atatgatattattaattccactgcctttggtagaggaaagtgctcaATAATAATCAATTGTTAAATTATGGTGCATTTCACTTCTGGATCTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgt                                               <  2:148573‑M2/103‑1 (MQ=255)                                                                                                    aaatgatattattaattccactgcctttggtagaggaaagtggtaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgt                                               <  2:148618‑M2/103‑1 (MQ=255)                                                                                                       tgatattattaattccactgcctttggtagaggaaagtgctaaATAGTAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttg                                            >  1:682882‑M2/44‑149 (MQ=255)                                                                                                        gttattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                           <  1:468271‑M2/107‑1 (MQ=255)                                                                                                              attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACCACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttg                                            <  1:578791‑M2/106‑1 (MQ=255)                                                                                                              attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACCACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttg                                            >  2:578791‑M2/37‑142 (MQ=255)                                                                                                                    tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                               <  1:319692‑M2/119‑1 (MQ=255)                                                                                                                    tccactccctttggtagaggaaaggtctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                               <  2:285184‑M2/119‑1 (MQ=255)                                                                                                                      cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                          <  1:256432‑M2/92‑1 (MQ=255)                                                                                                                      cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                          >  2:256432‑M2/29‑120 (MQ=255)                                                                                                                       actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                            <  2:599716‑M2/122‑1 (MQ=255)                                                                                                                          gcctttggtagaggaaagtgctaaATAATAATCACTTTTTAAAGTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                         <  1:396990‑M2/125‑1 (MQ=255)                                                                                                                             tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCACGCtat                      >  1:441075‑M2/22‑149 (MQ=255)                                                                                                                             tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                      <  1:289577‑M2/128‑1 (MQ=255)                                                                                                                                 gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATACTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt                  >  2:569199‑M2/18‑149 (MQ=255)                                                                                                                                      ggaaagtgctaaATAATAATCAATTGTTACATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGCCATGCTTAGCCAATCTCTATTTGATTTAATTGAAAGATGTTTGTTAAGGCATGGATGCCAGCTATCGattcggt             >  2:369001‑M2/13‑146 (MQ=255)                                                                                                                                       gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGTTTCTGAt            >  1:505970‑M2/12‑149 (MQ=255)                                                                                                                                        aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa           <  1:243539‑M2/139‑1 (MQ=255)                                                                                                                                                 aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa  >  2:547748‑M2/2‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_hsa_tpiA/1300553‑1300845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑