New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	3648348 =	53 (1.070)	4 (0.090)	4/260	6.6	7.5%	noncoding (55/84 nt)	agrB	antisense sRNA antitoxin for the DinQ toxin
	?	NC_000913_3_hsa_tpiA	3648375 =	50 (1.100)					noncoding (82/84 nt)	agrB	antisense sRNA antitoxin for the DinQ toxin
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGA  >  NC_000913_3_hsa_tpiA/3648202‑3648495                                                                                                                                                   |                                                                                                                                                    gTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTaaa                                                                                                                                                   >  2:483788/1‑149 (MQ=255)               taCCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAg                                                                                                                                     >  1:762174/1‑149 (MQ=255)                   gAAGTCCTCCCTCCGCCAGCTAAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAATTAAAAAACCGCCGCAAAGTCgg                                                                                                                                 >  2:26289/1‑149 (MQ=255)                         ctccctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTTTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACACAAAGTCCGtatgtt                                                                                                                           >  2:512212/1‑144 (MQ=255)                         ctccctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCAACTGGCTAATAATATTGCGCAATCCCTTAAGAAATCCTTCCCACCAGCCATAGCCACCGCCTCAGCCCTGGAACGCAAAAAACCGACTCAAAGTCGTattttt                                                                                                                           >  2:512395/1‑149 (MQ=255)                            cctccGCCAGCTGAAGAAATCGCTAATTCTTGCCATGTTAGCCCCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTACAAACCCGACGCAAAGTCGGTTTTTTTAc                                                                                                                        >  2:8387/1‑149 (MQ=255)                            cctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                        >  2:616847/1‑149 (MQ=255)                            cctccGCCAGCTGAAGAAATCGCTAATTAGTCGGAAGTTATGCACTGGGTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                        <  2:181729/149‑1 (MQ=21)                                     gCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGAtt                                                                                                               <  1:288378/149‑1 (MQ=255)                                          agaaATCGCTAATTCTTGCAATGTTAGCCACTTGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAc                                                                                                          <  1:89677/149‑1 (MQ=255)                                            aaaTCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAcct                                                                                                        >  1:529357/1‑149 (MQ=255)                                              aTCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATCCCGAcctcc                                                                                                      >  2:439398/1‑149 (MQ=255)                                                 gCTAATTCTTGCAATGTTAGCAACTGGCTAATAGTATTGAGCTGTTAGTTAAGAACTCTCTCAATCCAGACAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAATTCGGTTTTTGTACCTCCTGTTGCAGACCGACttt                                                                                                   >  2:472072/1‑149 (MQ=25)                                                   tAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCa                                                                                                 <  2:762174/149‑1 (MQ=255)                                                    aaTTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaa                                                                                                <  2:701201/149‑1 (MQ=255)                                                      ttCTTGCAATGGTAGCCACTGGGTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                              <  2:565739/149‑1 (MQ=255)                                                         ttGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGaa                                                                                           >  1:820603/1‑149 (MQ=255)                                                                gTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCaa                                                                                    >  1:607835/1‑149 (MQ=255)                                                                 ttAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAAc                                                                                   >  2:501285/1‑149 (MQ=255)                                                                       aCTGGCTAATAGTATTGAGCTGTTAGATAAGCACTCTCTCACTCCAGGCAGAGCCACCGACTGAGGGCTGGAAAGTAAAAAACCGACGCACAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCaaaa                                                                             >  2:692224/1‑149 (MQ=37)                                                                       aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACATCCTTCCTAAGTAATAGCCAACTCaaaa                                                                             >  2:218478/1‑149 (MQ=25)                                                                           gCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTca                                                                         <  1:754076/149‑1 (MQ=255)                                                                              aaTAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCacac                                                                      <  1:218478/149‑1 (MQ=255)                                                                               aTAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                              >  2:790503/1‑124 (MQ=255)                                                                               aTAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                              <  1:790503/124‑1 (MQ=255)                                                                                     ttGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTAc                                                               <  1:483788/149‑1 (MQ=255)                                                                                         gCTGGTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTc                                                           <  1:842836/149‑1 (MQ=255)                                                                                            gctAGATAAGAACTCTCGCACTCCAGCCCGAGCAACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTGTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCctc                                                        <  1:8387/147‑1 (MQ=255)                                                                                             ttAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCctct                                                       <  1:616847/149‑1 (MQ=255)                                                                                               aGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCACAATCCCCACATATTACCTTCCTCTGc                                                     >  2:564896/1‑149 (MQ=255)                                                                                               aGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGAGTCAGACCTCCTTTCAAATGAATCGCCCACTCAACATTCACACCTATTACCTTCCTCTGc                                                     >  2:765745/1‑149 (MQ=255)                                                                                                gATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCCCCCCTATTCCCTTCctcttcc                                                    >  2:286065/1‑146 (MQ=255)                                                                                                     gAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                        <  1:737330/76‑1 (MQ=255)                                                                                                     gAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                        >  2:737330/1‑76 (MQ=255)                                                                                                     gAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCCACTCAAAATTCACACCTATTACCTTCCTCTGCACttgc                                               >  2:269379/1‑147 (MQ=255)                                                                                                        ctctctCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTAcaca                                            >  1:529461/1‑149 (MQ=255)                                                                                                        ctctctCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTAcaca                                            >  2:518968/1‑149 (MQ=255)                                                                                                         tctctcACTCCAGCCAGAGCCACCAACTCAGGGCTGGACAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACAt                                           >  1:33567/1‑149 (MQ=255)                                                                                                             tcACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGt                                       >  1:53153/1‑149 (MQ=255)                                                                                                               aCTCCAGCCACAGGCACCACCTCAGCGCTGGAAAGTAAAAAACCGTCGCAAAGTCGGTTTTTTCACGTCCTGATTCAGACCTCCTTTCAAATTAACAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACAGTAGTTa                                     <  2:68316/149‑1 (MQ=37)                                                                                                                cTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCCCACCTATTACCTTCCTCTTCACTTACACATTCGTTaa                                    >  1:88341/1‑149 (MQ=255)                                                                                                                 tCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAg                                   >  2:290686/1‑149 (MQ=255)                                                                                                                             caccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGttt                       <  1:275493/149‑1 (MQ=255)                                                                                                                              accaACGCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGtttt                      <  1:387123/149‑1 (MQ=255)                                                                                                                                 aaCTCAGGCCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGa                   <  1:658844/149‑1 (MQ=255)                                                                                                                                  aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCa                                                    <  2:534123/115‑1 (MQ=255)                                                                                                                                  aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCa                                                    >  1:534123/1‑115 (MQ=255)                                                                                                                                      aGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaaa                                                                                               >  2:68075/1‑68 (MQ=255)                                                                                                                                      aGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaaa                                                                                               <  1:68075/68‑1 (MQ=255)                                                                                                                                        ggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATcc            >  1:88269/1‑149 (MQ=255)                                                                                                                                              aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGc                                                                                       <  2:177547/68‑1 (MQ=255)                                                                                                                                              aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGc                                                                                       >  1:177547/1‑68 (MQ=255)                                                                                                                                                  tAAAAAACCGACGCAAAGTCGGTTTGTTTACGTCCTGATTCAGACCTCCTTTCAAACGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAaga  >  1:788847/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGA  >  NC_000913_3_hsa_tpiA/3648202‑3648495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.