New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	3648348 =	53 (1.070)	4 (0.090)	4/260	6.6	7.5%	noncoding (55/84 nt)	agrB	antisense sRNA antitoxin for the DinQ toxin
	?	NC_000913_3_hsa_tpiA	3648375 =	50 (1.100)					noncoding (82/84 nt)	agrB	antisense sRNA antitoxin for the DinQ toxin
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACCTGCAACAATCAG  >  NC_000913_3_hsa_tpiA/3648229‑3648518                                                                                                                                                   |                                                                                                                                                cctccGCCAGCTGAAGAAATCGCTAATTCTTGCCATGTTAGCCCCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTACAAACCCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                               >  2:8387/1‑149 (MQ=255) cctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                               >  2:616847/1‑149 (MQ=255) cctccGCCAGCTGAAGAAATCGCTAATTAGTCGGAAGTTATGCACTGGGTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                               <  2:181729/149‑1 (MQ=21)          gCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGAtt                                                                                                                                      <  1:288378/149‑1 (MQ=255)               agaaATCGCTAATTCTTGCAATGTTAGCCACTTGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAc                                                                                                                                 <  1:89677/149‑1 (MQ=255)                 aaaTCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAcct                                                                                                                               >  1:529357/1‑149 (MQ=255)                   aTCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATCCCGAcctcc                                                                                                                             >  2:439398/1‑149 (MQ=255)                      gCTAATTCTTGCAATGTTAGCAACTGGCTAATAGTATTGAGCTGTTAGTTAAGAACTCTCTCAATCCAGACAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAATTCGGTTTTTGTACCTCCTGTTGCAGACCGACttt                                                                                                                          >  2:472072/1‑149 (MQ=25)                        tAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCa                                                                                                                        <  2:762174/149‑1 (MQ=255)                         aaTTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaa                                                                                                                       <  2:701201/149‑1 (MQ=255)                           ttCTTGCAATGGTAGCCACTGGGTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                                                     <  2:565739/149‑1 (MQ=255)                              ttGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGaa                                                                                                                  >  1:820603/1‑149 (MQ=255)                                     gTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCaa                                                                                                           >  1:607835/1‑149 (MQ=255)                                      ttAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAAc                                                                                                          >  2:501285/1‑149 (MQ=255)                                            aCTGGCTAATAGTATTGAGCTGTTAGATAAGCACTCTCTCACTCCAGGCAGAGCCACCGACTGAGGGCTGGAAAGTAAAAAACCGACGCACAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCaaaa                                                                                                    >  2:692224/1‑149 (MQ=37)                                            aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACATCCTTCCTAAGTAATAGCCAACTCaaaa                                                                                                    >  2:218478/1‑149 (MQ=25)                                                gCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTca                                                                                                <  1:754076/149‑1 (MQ=255)                                                   aaTAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCacac                                                                                             <  1:218478/149‑1 (MQ=255)                                                    aTAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                                                     >  2:790503/1‑124 (MQ=255)                                                    aTAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                                                     <  1:790503/124‑1 (MQ=255)                                                          ttGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTAc                                                                                      <  1:483788/149‑1 (MQ=255)                                                              gCTGGTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTc                                                                                  <  1:842836/149‑1 (MQ=255)                                                                 gctAGATAAGAACTCTCGCACTCCAGCCCGAGCAACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTGTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCctc                                                                               <  1:8387/147‑1 (MQ=255)                                                                  ttAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCctct                                                                              <  1:616847/149‑1 (MQ=255)                                                                    aGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCACAATCCCCACATATTACCTTCCTCTGc                                                                            >  2:564896/1‑149 (MQ=255)                                                                    aGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGAGTCAGACCTCCTTTCAAATGAATCGCCCACTCAACATTCACACCTATTACCTTCCTCTGc                                                                            >  2:765745/1‑149 (MQ=255)                                                                     gATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCCCCCCTATTCCCTTCctcttcc                                                                           >  2:286065/1‑146 (MQ=255)                                                                          gAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                               <  1:737330/76‑1 (MQ=255)                                                                          gAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                               >  2:737330/1‑76 (MQ=255)                                                                          gAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCCACTCAAAATTCACACCTATTACCTTCCTCTGCACttgc                                                                      >  2:269379/1‑147 (MQ=255)                                                                             ctctctCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTAcaca                                                                   >  2:518968/1‑149 (MQ=255)                                                                             ctctctCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTAcaca                                                                   >  1:529461/1‑149 (MQ=255)                                                                              tctctcACTCCAGCCAGAGCCACCAACTCAGGGCTGGACAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACAt                                                                  >  1:33567/1‑149 (MQ=255)                                                                                  tcACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGt                                                              >  1:53153/1‑149 (MQ=255)                                                                                    aCTCCAGCCACAGGCACCACCTCAGCGCTGGAAAGTAAAAAACCGTCGCAAAGTCGGTTTTTTCACGTCCTGATTCAGACCTCCTTTCAAATTAACAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACAGTAGTTa                                                            <  2:68316/149‑1 (MQ=37)                                                                                     cTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCCCACCTATTACCTTCCTCTTCACTTACACATTCGTTaa                                                           >  1:88341/1‑149 (MQ=255)                                                                                      tCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAg                                                          >  2:290686/1‑149 (MQ=255)                                                                                                  caccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGttt                                              <  1:275493/149‑1 (MQ=255)                                                                                                   accaACGCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGtttt                                             <  1:387123/149‑1 (MQ=255)                                                                                                      aaCTCAGGCCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGa                                          <  1:658844/149‑1 (MQ=255)                                                                                                       aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCa                                                                           <  2:534123/115‑1 (MQ=255)                                                                                                       aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCa                                                                           >  1:534123/1‑115 (MQ=255)                                                                                                           aGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaaa                                                                                                                      <  1:68075/68‑1 (MQ=255)                                                                                                           aGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaaa                                                                                                                      >  2:68075/1‑68 (MQ=255)                                                                                                             ggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATcc                                   >  1:88269/1‑149 (MQ=255)                                                                                                                   aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGc                                                                                                              >  1:177547/1‑68 (MQ=255)                                                                                                                   aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGc                                                                                                              <  2:177547/68‑1 (MQ=255)                                                                                                                       tAAAAAACCGACGCAAAGTCGGTTTGTTTACGTCCTGATTCAGACCTCCTTTCAAACGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAaga                         >  1:788847/1‑149 (MQ=255)                                                                                                                         aaaaaCCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAagaga                       <  1:138562/149‑1 (MQ=255)                                                                                                                          aaaaCCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAGTAGCTGACTCGTGATTTGCACCTATTACCTTACTCTGCATTTACACATTCGTTAAGTTATATATGTTTTTGACTTATTCGCTTCGAtgagag                      >  2:268099/1‑149 (MQ=255)                                                                                                                                         tCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAagag                        >  1:37790/1‑132 (MQ=255)                                                                                                                                         tCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAagag                        <  2:37790/132‑1 (MQ=255)                                                                                                                                          cGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACCTGcaacaa      <  1:290686/149‑1 (MQ=255)                                                                                                                                            gTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACCTGCAACAATc    <  1:512395/149‑1 (MQ=255)                                                                                                                                            gTTTTTTCACGCTCTGATTCAGACCTCCTCTCCACTGACTCGCCAACTCCAAAGTCACACCTATTACCTTCCTCTGCACTGACACCTGCGTCGATTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACCTGCAACAATc    <  1:512212/149‑1 (MQ=255)                                                                                                                                             ttttttttCTTTCTGATTCAAACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACCTGCAACAATCa   >  2:75186/1‑149 (MQ=255)                                                                                                                                              tattttACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACCTGCAACAATCAg  <  2:228466/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                CCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACCTGCAACAATCAG  >  NC_000913_3_hsa_tpiA/3648229‑3648518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.