New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 79980	33 (0.790)	4 (0.100)	4/268	5.3	11.9%	coding (885/1401 nt)	leuC	3‑isopropylmalate dehydratase large subunit
	?	NC_000913_3_hsa_tpiA	= 79994	28 (0.710)					coding (871/1401 nt)	leuC	3‑isopropylmalate dehydratase large subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGACGGCCTTTGACATAG  >  NC_000913_3_hsa_tpiA/79846‑80142                                                                                                                                                     |                                                                                                                                                     tCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGcc                                                                                                                                                      <  2:97527/149‑1 (MQ=255)   gATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGcccc                                                                                                                                                    >  2:15288/1‑149 (MQ=255)              cGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAg                                                                                                                                                  >  2:680535/1‑140 (MQ=255)              cGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAg                                                                                                                                                  <  1:680535/140‑1 (MQ=255)                     cGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGaaa                                                                                                                                  <  1:713914/149‑1 (MQ=255)                       gAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTTCTTTTTCGCGATCAACCGGAGCGGCAAACATAACCGGATCGGGAATATTGTCGTTCACGGAAATCACCCCGCCGGGAGTGGTGCCCACGGTGACCTGCggccgaatt                                                                                                                                >  2:583205/1‑142 (MQ=255)                         aTACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATttc                                                                                                                              <  1:192851/149‑1 (MQ=255)                           aCCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCGCGGAAATCACCTGGCCGGGATTGGTGCCCCAGGGGACCTGCGGTGAAATttctt                                                                                                                            >  2:466949/1‑149 (MQ=255)                              cGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGCTCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGattt                                                                                                                                           >  2:436391/1‑128 (MQ=255)                              cGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGattt                                                                                                                                           <  1:436391/131‑4 (MQ=255)                               gggttCAGCCCCATAGAGCCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGc                                                                                                                        <  1:503835/146‑1 (MQ=255)                                  ttCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATGACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTg                                                                                                                     <  1:476843/149‑1 (MQ=255)                                           atatAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGAc                                                                                                            <  1:15288/149‑1 (MQ=255)                                                      cgcTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGa                                                                                                 <  2:449345/149‑1 (MQ=255)                                                         tttttCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAg                                                                                              >  1:384715/1‑149 (MQ=255)                                                                      gcgcgTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCATtcgtc                                                                                 >  1:456415/1‑149 (MQ=255)                                                                              aaCCGGACCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCa                                                                         <  1:166284/149‑1 (MQ=255)                                                                                      cGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACTGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTc                                                                 <  1:323490/149‑1 (MQ=255)                                                                                      cGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTc                                                                 <  1:365429/149‑1 (MQ=255)                                                                                      cGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTc                                                                 <  2:187720/149‑1 (MQ=255)                                                                                      cGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCAAGGTGACCTGCAGCGAAATGTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTc                                                                 <  1:531068/149‑1 (MQ=255)                                                                                        gCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCa                                                               >  2:197236/1‑149 (MQ=255)                                                                                           aaCGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCTCCTGACCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTa                                                            <  1:545114/149‑1 (MQ=255)                                                                                            aCGAAGCCGGATCGGGAAGATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGAGACGGAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACGACGGTCTCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAg                                                           <  1:348841/149‑1 (MQ=255)                                                                                             cGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTNGTCGGTTTGCAGGGTTTTCCAGTAgg                                                          >  1:574334/1‑149 (MQ=255)                                                                                                       ccGGGAATGTTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGcgtc                                                <  2:551112/148‑1 (MQ=255)                                                                                                                  gTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCtt                                     >  2:660899/1‑149 (MQ=255)                                                                                                                             aaaTCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGgc                          <  1:15558/149‑1 (MQ=255)                                                                                                                                      ggCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGa                 <  1:548557/149‑1 (MQ=255)                                                                                                                                       gCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGAc                <  1:197236/149‑1 (MQ=255)                                                                                                                                       gCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGCTTTTCCAGTAGGAAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGAc                <  1:30791/149‑1 (MQ=255)                                                                                                                                              ttGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGACGGCCttt         <  1:232123/149‑1 (MQ=255)                                                                                                                                                 gTGCCCCATGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGACGGCCTTTGAc      <  2:630320/149‑1 (MQ=255)                                                                                                                                                     cccAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGACGGCCTTTGACATAg  >  1:390901/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTTCGTCGGTTTGCAGGGTTTTCCAGTAGGCAACGGCGTCGTCGAAATCTTTGCCTTTCGGCGCATGCAGACGGCCTTTGACATAG  >  NC_000913_3_hsa_tpiA/79846‑80142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.