New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	127595 =	51 (0.710)	6 (0.090)	6/270	8.9	10.7%	intergenic (+8/‑317)	aceF/lpd	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2/dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase
	?	NC_000913_3_hsa_tpiA	127615 =	52 (0.770)					intergenic (+28/‑297)	aceF/lpd	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2/dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCT  >  NC_000913_3_hsa_tpiA/127470‑127762                                                                                                                                                  |                                                                                                                                                    tCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGCCCCAACGCCCGGCttttt                                                                                                                                                  >  2:339969/1‑149 (MQ=255)   tGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTc                                                                                                                                                <  1:485662/149‑1 (MQ=255)    gATGCTGCCGATTTCTCTCGCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCt                                                                                                                                               <  1:399521/149‑1 (MQ=255)     acgctgcCGCTTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTg                                                                                                                                              <  2:622864/147‑1 (MQ=255)         tgcCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACCCGCTGTCTGACATTCGCCGTCTGCTGGGGTAAGTAAAAGAGCCGGCACCACGACCGGCTTTTTTCTGGtca                                                                                                                                          >  1:1137452/1‑147 (MQ=255)         tgcCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTGCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATTTAAGTAAAAGAGCCGGCCCAACGGACGGCTTTTTTCTGGTaa                                                                                                                                          >  1:526025/1‑149 (MQ=255)            cGATTTCTCTCTCCTTCGACCACAGCGTGATCGACGGTGCTGAAGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTGGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCGGGCTTTTTTCTGGTAAtct                                                                                                                                       >  1:45264/1‑149 (MQ=255)                   tctctcCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATCCGCCGTCTGGTGATGTAAGTACAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCCCATGAAt                                                                                                                                >  2:594239/1‑149 (MQ=255)                        ccTTCGACCGCCGCTTGCTCGCACGTGCTCATGCTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAATAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTAtt                                                                                                                           <  1:186229/149‑1 (MQ=255)                         cTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGGATGTATTg                                                                                                                          >  1:1127703/1‑149 (MQ=255)                         cTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTg                                                                                                                          <  2:147270/149‑1 (MQ=255)                          ttCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGa                                                                                                                         >  2:646173/1‑149 (MQ=255)                                 accGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGttatt                                                                                                                  >  1:625452/1‑149 (MQ=255)                                  ccGCGTGATCGACGTTGCTTACGGTGCCCGTTTCATTACCATCATTAACAACCCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGttatta                                                                                                                 <  1:939833/149‑1 (MQ=255)                                   cgcgTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAg                                                                                                                >  1:988377/1‑149 (MQ=255)                                   cgcgTGATCCACGGTGCCGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAg                                                                                                                <  2:170069/149‑1 (MQ=255)                                      gTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGa                                                                                                             <  1:46837/149‑1 (MQ=255)                                       tggtCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGaa                                                                                                            <  1:110005/146‑1 (MQ=255)                                       tGATCGACGGTGCTGCTGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGaa                                                                                                            <  1:71109/149‑1 (MQ=255)                                                            cccGTTTCATTACCATCATTAACAACACGCAGTCTGACATTCGCCGTTTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGttt                                                                                       <  1:480583/149‑1 (MQ=255)                                                             ccGTTTCATTACCATCATTAACAACACGCTGTCTGGCATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAAACAAATAGGTTGACATttg                                                                                      >  2:816527/1‑149 (MQ=255)                                                             ccGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTttg                                                                                      >  2:844291/1‑149 (MQ=255)                                                               ggttCATTACCCTCACTAACAACACGCTGTCTGACATGCGCCGTCTTGTGATGGAAGGAAAAGAGCCGGCCCAACGGCCGGCTTTTTCCTGGTAATCGCATGAATTTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTttgtt                                                                                    <  2:712073/147‑1 (MQ=255)                                                                tttCATTACCATCATTAATAGCACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTGAGGTAATCTCATTAATCTATAGCGGATATTAGCGAATAGACAAATCGGTTGCtgtttgttt                                                                                   >  2:983615/1‑148 (MQ=255)                                                                        ccATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTaaaaa                                                                           >  2:915629/1‑149 (MQ=255)                                                                          atcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTACAAAtt                                                                         >  1:326764/1‑149 (MQ=255)                                                                          atcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAAtt                                                                         <  1:716288/149‑1 (MQ=255)                                                                                 acaacaCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTCAAAATTGTTAACa                                                                  >  1:901065/1‑149 (MQ=255)                                                                                  caacaCGCTGTCTGACATTCGCCGTCTTGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACaa                                                                 <  1:961419/149‑1 (MQ=255)                                                                                  caacaCGCTGTCTGACATTCGCCGTCTCGTGATGTAAGTCAAAGAGCCATCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGGGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAACTTGTTAACaa                                                                 >  1:178894/1‑149 (MQ=255)                                                                                   aacaCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGttatt                                                                                                                  >  1:1053048/1‑99 (MQ=255)                                                                                   aacaCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGttatt                                                                                                                  <  2:1053048/99‑1 (MQ=255)                                                                                    acacGCTTTCTGACAGTCGCCGTCTGGTGATTTAAGTGAAAGCGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAAtt                                                               <  1:629786/149‑1 (MQ=255)                                                                                      acGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAAtttt                                                             <  2:901750/149‑1 (MQ=255)                                                                                          tGTCTGACATTCGCCGTCTGGGGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAAtttttaaa                                                         <  1:1192904/149‑5 (MQ=255)                                                                                            tCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATCGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTaaaa                                                       <  1:979944/149‑1 (MQ=255)                                                                                              tGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTttgtt                                                                                    <  1:1107153/118‑1 (MQ=255)                                                                                              tGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTttgtt                                                                                    >  2:1107153/1‑118 (MQ=255)                                                                                              tGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATa                                                     >  2:480679/1‑149 (MQ=255)                                                                                               gACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATAc                                                    <  2:163587/149‑1 (MQ=255)                                                                                                       ccGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCAACGGAt                                            >  1:115869/1‑149 (MQ=255)                                                                                                           tttgTGATGGAAGGAAAAGGCGCGGCCGGACTGGCGGCTTTTTTCGGGTAATCTTATGAATGTATTGAGGTTAGTAGCGAATAGACAAATTGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGaa                                        <  2:200054/146‑1 (MQ=255)                                                                                                               tGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCCTGAATTTGTTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGAc                                    >  1:668143/1‑149 (MQ=255)                                                                                                                    taagtaaAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggt                               >  2:491025/1‑149 (MQ=255)                                                                                                                        taaAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggtggtg                           <  1:915629/149‑1 (MQ=255)                                                                                                                        taaAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggtggtg                           <  2:447025/149‑1 (MQ=255)                                                                                                                            agagCCGGCCCAACGGTCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTa                       >  2:150944/1‑149 (MQ=255)                                                                                                                              agCCGGCCCAAGGGCCGGCGTTTTCCTGGGAATGTGATGAATGTATTGAGGTTATGAGGGAATAGGCAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTGACAATTTTGTAAAATACCGACGGATGGAACGACCCGGTGGTGGGTAgg                     <  2:42195/149‑1 (MQ=255)                                                                                                                              agCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTCTTGGGGTTAATATCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAgg                     <  1:662883/149‑1 (MQ=255)                                                                                                                              agCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAgg                     >  2:235349/1‑149 (MQ=255)                                                                                                                                       aaCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACtt            >  1:1066574/1‑149 (MQ=255)                                                                                                                                        aCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTc           <  1:844291/149‑1 (MQ=255)                                                                                                                                           gccggcTTTTATCTGGTACTCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAACATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTcaca        <  1:403304/149‑1 (MQ=255)                                                                                                                                             cggcTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCAAATa      >  2:812195/1‑149 (MQ=255)                                                                                                                                               gtttttttttGGTAATCTAATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATAcc    <  1:548147/147‑1 (MQ=255)                                                                                                                                                 ttttttCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCt  >  1:104618/1‑149 (MQ=255)                                                                                                                                                 tttttgCTGGTAATCTCAGGAATGTACTGATGTTAGTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCt  <  1:1158465/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCT  >  NC_000913_3_hsa_tpiA/127470‑127762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.