New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 213451	30 (1.220)	3 (0.130)	3/270	3.2	9.9%	coding (1121/1299 nt)	tilS	tRNA(Ile)‑lysidine synthetase
	?	NC_000913_3_hsa_tpiA	= 213488	26 (1.110)					coding (1158/1299 nt)	tilS	tRNA(Ile)‑lysidine synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGC  >  NC_000913_3_hsa_tpiA/213310‑213596                                                                                                                                               |                                                                                                                                                  ttCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGAGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTCACGGCGGACGTAAg                                                                                                                                            >  2:200817/1‑149 (MQ=255) ttCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGAGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCCG‑GAACGATTGCATTGCGCCAGGAGTGCTGCTTGTTGTCAGGCGTAACGGCGGACGTAAg                                                                                                                                            >  1:82220/1‑149 (MQ=255) ttCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCATAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGAGTGCTGCATATTGTCGGGCGTAACGCCGGCGGTAAg                                                                                                                                            >  2:49299/1‑149 (MQ=255)     aCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaa                                                                                                                                        >  1:266680/1‑149 (MQ=255)               ttACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTg                                                                                                                              <  1:357718/149‑1 (MQ=255)                tACCGGCGGGGCTGGGAAGTTGACAGGCTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCCGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTgg                                                                                                                             <  1:309086/149‑1 (MQ=255)                tACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTgg                                                                                                                             <  1:200324/149‑1 (MQ=255)                          gCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg                                                                                                                   <  1:228884/149‑1 (MQ=255)                              ggAAGTGTACAGCTTAATGCGGGAGGCGATATTCCCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGt                                                                                                               >  2:340524/1‑149 (MQ=255)                                   tgtACAGCTTAATGCGGGAGGCGATCTTCGCCCTCCGCGTGCAGACGAAGCGGTCGGCGGGCG‑GTGCTAAGCGGCAGGATTGCTGCATATGGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGAAAGAGCTGGGAGGgccgc                                                                                                          >  2:103190/1‑149 (MQ=255)                                   tgtACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgccgc                                                                                                          <  1:52583/149‑1 (MQ=255)                                     tACAGCTTACTGCGTGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCCAAAGAAAATCTGGCAAGAGCTGAGGGTGcccccg                                                                                                        >  2:113036/1‑149 (MQ=255)                                      aCAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTTAGCTAAAATCAATCTGGCAAGGGCTGGGCGTACCGCCGt                                                                                                       >  2:248717/1‑149 (MQ=255)                                      aCAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGt                                                                                                       >  1:212383/1‑149 (MQ=255)                                        aGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTgg                                                                                                     >  1:304881/1‑149 (MQ=255)                                                gCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGa                                                                                             <  1:357321/149‑1 (MQ=255)                                                               cGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCActgct                                                                              >  2:236887/1‑149 (MQ=255)                                                                 cccTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGt                                                                            >  2:32950/1‑149 (MQ=255)                                                                       gcttacAGACGAAGCGGTCAGCCTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGTTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATg                                                                      <  1:378698/144‑1 (MQ=255)                                                                                    gCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGAt                                                         >  2:138222/1‑149 (MQ=255)                                                                                               gcg‑tTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTAt                                                                       <  1:142998/124‑1 (MQ=255)                                                                                               gcg‑tTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTAt                                                                       >  2:142998/1‑124 (MQ=255)                                                                                                 g‑tTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAggg                                            <  1:296870/149‑1 (MQ=255)                                                                                                 g‑tTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGGGACACCACGCCACTGCTTTTTTATGGCGAAACGCGGGTTGCGGCGGCAggg                                            >  1:51929/1‑149 (MQ=255)                                                                                                            gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGa                                  >  1:257319/1‑149 (MQ=255)                                                                                                                         cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGt                                                                                               <  2:140848/75‑1 (MQ=255)                                                                                                                         cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGt                                                                                               >  1:140848/1‑75 (MQ=255)                                                                                                                                     cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTga         <  1:113036/149‑1 (MQ=255)                                                                                                                                          cggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGACaaag    <  2:330053/149‑3 (MQ=255)                                                                                                                                           ggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCCGGAAGGTGTGGCTGAAGGTGAGAATgg   >  1:273182/1‑149 (MQ=255)                                                                                                                                            gcggACGTAAGCTAAAGAAAATCTGGCAAGGGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGc  <  2:2371/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  TTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCG‑TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGC  >  NC_000913_3_hsa_tpiA/213310‑213596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.