New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 213451	30 (1.220)	3 (0.130)	3/270	3.2	9.9%	coding (1121/1299 nt)	tilS	tRNA(Ile)‑lysidine synthetase
	?	NC_000913_3_hsa_tpiA	= 213488	26 (1.110)					coding (1158/1299 nt)	tilS	tRNA(Ile)‑lysidine synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGT  >  NC_000913_3_hsa_tpiA/213344‑213626                                                                                                                                                 |                                                                                                                                           tgtACAGCTTAATGCGGGAGGCGATCTTCGCCCTCCGCGTGCAGACGAAGCGGTCGGCGGGCGGTGCTAAGCGGCAGGATTGCTGCATATGGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGAAAGAGCTGGGAGGgccgc                                                                                                                                        >  2:103190/1‑149 (MQ=255) tgtACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgccgc                                                                                                                                        <  1:52583/149‑1 (MQ=255)   tACAGCTTACTGCGTGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCCAAAGAAAATCTGGCAAGAGCTGAGGGTGcccccg                                                                                                                                      >  2:113036/1‑149 (MQ=255)    aCAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTTAGCTAAAATCAATCTGGCAAGGGCTGGGCGTACCGCCGt                                                                                                                                     >  2:248717/1‑149 (MQ=255)    aCAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGt                                                                                                                                     >  1:212383/1‑149 (MQ=255)      aGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTgg                                                                                                                                   >  1:304881/1‑149 (MQ=255)              gCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGa                                                                                                                           <  1:357321/149‑1 (MQ=255)                             cGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCActgct                                                                                                            >  2:236887/1‑149 (MQ=255)                               cccTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGt                                                                                                          >  2:32950/1‑149 (MQ=255)                                     gcttacAGACGAAGCGGTCAGCCTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGTTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATg                                                                                                    <  1:378698/144‑1 (MQ=255)                                                  gCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGAt                                                                                       >  2:138222/1‑149 (MQ=255)                                                             gcgtTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTAt                                                                                                     >  2:142998/1‑124 (MQ=255)                                                             gcgtTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTAt                                                                                                     <  1:142998/124‑1 (MQ=255)                                                               gtTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAggg                                                                          <  1:296870/149‑1 (MQ=255)                                                               gtTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGGGACACCACGCCACTGCTTTTTTATGGCGAAACGCGGGTTGCGGCGGCAggg                                                                          >  1:51929/1‑149 (MQ=255)                                                                         gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGa                                                                >  1:257319/1‑149 (MQ=255)                                                                                      cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGt                                                                                                                             >  1:140848/1‑75 (MQ=255)                                                                                      cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGt                                                                                                                             <  2:140848/75‑1 (MQ=255)                                                                                                  cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTga                                       <  1:113036/149‑1 (MQ=255)                                                                                                       cggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGACaaag                                  <  2:330053/149‑3 (MQ=255)                                                                                                        ggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCCGGAAGGTGTGGCTGAAGGTGAGAATgg                                 >  1:273182/1‑149 (MQ=255)                                                                                                         gcggACGTAAGCTAAAGAAAATCTGGCAAGGGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGc                                <  2:2371/149‑1 (MQ=255)                                                                                                              cGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTACTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAg                           <  1:200817/149‑1 (MQ=255)                                                                                                                   gCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTg                      <  1:32950/149‑1 (MQ=255)                                                                                                                     tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTACGTTTTGTc                    >  1:304017/1‑149 (MQ=255)                                                                                                                        aGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTgg                 <  1:327738/149‑1 (MQ=255)                                                                                                                          aaaaTCTGGCAAGAGCTGGGCATGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTCTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAACAAGAAAGGTCTTAAGGTGATAATGACGTAAGTTTTGTCTGGCa               >  2:379585/1‑149 (MQ=255)                                                                                                                                   gaaGAGCTGGGCGTGACGGCTGATCTACTTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt      <  2:304017/148‑1 (MQ=255)                                                                                                                                   cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt      >  1:85710/1‑149 (MQ=255)                                                                                                                                      gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGCTTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGCGGATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg   >  2:115235/1‑149 (MQ=255)                                                                                                                                       agCGGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTAGGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGt  <  2:82220/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                           TGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGT  >  NC_000913_3_hsa_tpiA/213344‑213626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.