New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	3596422 =	23 (0.680)	5 (0.160)	5/266	3.7	17.3%	intergenic (‑19/+29)	livH/livK	branched‑chain amino acid ABC transporter permease/leucine transporter subunit
	?	NC_000913_3_hsa_tpiA	3596443 =	26 (0.810)					intergenic (‑40/+8)	livH/livK	branched‑chain amino acid ABC transporter permease/leucine transporter subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGC  >  NC_000913_3_hsa_tpiA/3596275‑3596570                                                                                                                                                      |                                                                                                                                                     ctGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGTTGTAGCCGATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAACAACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAAccc                                                                                                                                                     <  2:129806/148‑1 (MQ=255)         aGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGAAGG‑‑ATCCGCGGCGGTATGTACCCCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCAt                                                                                                                                             <  1:265686/149‑1 (MQ=255)               tCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACg                                                                                                                                       <  2:326458/149‑1 (MQ=255)                         aaTGGCGTAAACCAGGGTGTAGCCGATGGGTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGGCGACGTTAAACAGCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGga                                                                                                                             <  2:367208/149‑1 (MQ=255)                            gCCGTAAACCATGGTGTAGCCGATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGgatga                                                                                                                          >  2:406407/1‑149 (MQ=255)                                     cATGGTGTAGCCGATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGc                                                                                                                 >  1:225602/1‑149 (MQ=255)                                     cATGGTGTAGCCGATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGc                                                                                                                 >  2:106596/1‑149 (MQ=255)                                                 gATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGa                                                                                                     >  1:544279/1‑149 (MQ=255)                                                            gc‑gc‑GTAGGTACCGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTGTACGGGCGGAGGAATCATCACTTGGCTGCCGTGGATGAACCGTCGGCGt                                                                                          <  1:592604/149‑1 (MQ=255)                                                                     ggTACTGCCCAGCGTGACGCCGTCAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGACCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTg                                                                                   <  1:520544/149‑1 (MQ=255)                                                                          tGCCCAGCGTGCCGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCTCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGa                                                                              <  1:306755/149‑1 (MQ=255)                                                                             ccAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGacac                                                                           >  1:274216/1‑149 (MQ=255)                                                                                     aCGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAaaatca                                                                   >  1:519237/1‑149 (MQ=255)                                                                                       gCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAaaatcaaa                                                                 <  2:519237/149‑1 (MQ=255)                                                                                                 aTCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGa                                                       >  2:264417/1‑149 (MQ=255)                                                                                                    tgctgcAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGgatgat                                                                                                                         <  2:3900/80‑1 (MQ=255)                                                                                                    tgctgcAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGgatgat                                                                                                                         >  1:3900/1‑80 (MQ=255)                                                                                                     gctgcAAGCAAAACAACAACTGCAAAGCCATAAGGATACCTTTCTAATTCCGTTGGCTTGTTACGTGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTGGGCGTGCCACTGGAAGCAACCAAAATCAATTCCCTTAAGATCGc                                                   >  2:205734/1‑149 (MQ=255)                                                                                                         cAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCttt                                               >  2:305123/1‑149 (MQ=255)                                                                                                                tACAAAAACTGCTTAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATcc                                        <  2:175338/149‑1 (MQ=255)                                                                                                                                       gTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATAACGgt                 >  1:309740/1‑149 (MQ=255)                                                                                                                                           tcTTTCTAAGCCCGCCCCCATTTTACGGGCGGTTGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGttt             <  1:352828/148‑1 (MQ=255)                                                                                                                                                  aaaCCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGt      <  1:305123/149‑1 (MQ=255)                                                                                                                                                      ccgcccgcATTTTACGTGCGGGGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCCCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGc  <  1:491516/149‑1 (MQ=255)                                                                                                                                                      ccgcccgcATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTACGATCGCCTTTTTCCTCACAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGc  >  1:529533/1‑149 (MQ=255)                                                                                                                                                      ccgcccgcATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGc  >  1:160758/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGC‑GC‑GTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGC  >  NC_000913_3_hsa_tpiA/3596275‑3596570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.