New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 15310 =50 (1.190)3 (0.110)
+CTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGG
3/184 4.4 8.7% intergenic (+12/‑135) dnaJ/insL1 chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
?NC_000913_3_hsa_tpiA 2514263 = 47 (1.120)intergenic (+19/‑68) nupC/insL1 nucleoside (except guanosine) transporter/IS186 transposase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCA  >  NC_000913_3_hsa_tpiA/15163‑15458
                                                                                                                                                   |                                                                                                                                                    
ccTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGc                                                                                                                                                     >  1:572198/1‑149 (MQ=255)
 cTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGcc                                                                                                                                                    >  1:567349/1‑149 (MQ=255)
    aaCGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGc                                                                                                                                                 <  2:711001/149‑1 (MQ=255)
                aaaCAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGc                                                                                                                                     >  1:660987/1‑149 (MQ=255)
                  aCAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGTCt                                                                                                                                   >  1:108817/1‑149 (MQ=255)
                  aCAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCt                                                                                                                                   <  2:224182/149‑1 (MQ=255)
                     gctgctGCAAGAGCTGCAAGAAAGCCTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCACGGGCAGGcttttg                                                                                                                                <  1:235662/149‑6 (MQ=255)
                            caagagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTaaaact                                                                                                                         >  2:131449/1‑147 (MQ=255)
                            caagagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAAt                                                                                                                         <  1:215580/149‑1 (MQ=255)
                               gcgctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg                                                                                                                      <  1:431302/147‑1 (MQ=255)
                               gagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg                                                                                                                      <  1:131449/149‑1 (MQ=255)
                                    gcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGTTAAAAATAGGGTGCg                                                                                                                 >  1:203212/1‑149 (MQ=255)
                                       agaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTg                                                                                                              >  1:234048/1‑149 (MQ=255)
                                         aaaGCGTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCGTCTTTGATGGTGTGAAGAAGTTGTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGaa                                                                                                            <  2:208756/149‑1 (MQ=255)
                                          aaGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAACGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAg                                                                                                           <  2:572198/149‑1 (MQ=255)
                                           aGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCCACATTGATCCATGCCTGGGTAAAAATAGGGTGCGTTGAAGa                                                                                                          <  1:367253/149‑1 (MQ=255)
                                              ttCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGatat                                                                                                       <  2:234048/149‑1 (MQ=255)
                                              ttCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGatat                                                                                                       >  1:679504/1‑149 (MQ=255)
                                                cGGTGCCCCAACCGGGGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCTGCTAATCTCCCCAGAAGCCTGTCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGc                                                                                                     <  2:309968/149‑1 (MQ=255)
                                                  gTGGCCCAACGGGCGAGCACAACAGTCCTCGCTCAAAGAGCTTCGTTGATGGTGTTAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGa                                                                                                   <  2:695787/149‑1 (MQ=255)
                                                   tGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGATGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAg                                                                                                  <  1:512643/149‑1 (MQ=255)
                                                      cccAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCAc                                                                                               <  2:235148/149‑1 (MQ=255)
                                                       ccAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCAcc                                                                                              >  1:185287/1‑149 (MQ=255)
                                                              gCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGCAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAg                                                                                       <  1:688561/149‑1 (MQ=255)
                                                              gCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAg                                                                                       >  2:334667/1‑149 (MQ=255)
                                                                gAGCACAACAGCCCGGGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTg                                                                                     <  2:166467/149‑1 (MQ=255)
                                                                      aacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCgggg                                                                               >  1:80805/1‑149 (MQ=255)
                                                                       acaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGTCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGa                                                                              <  1:249996/149‑1 (MQ=255)
                                                                       acaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGa                                                                              <  1:20213/149‑1 (MQ=255)
                                                                          gCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGCCCCGCTAACCTCCCAAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAAGAGGGTGCGTTGAAGATATGCGAGCATCTGTAAAGTGGCGGGGATCa                                                                           <  2:421468/149‑1 (MQ=255)
                                                                             cgcgCTCAAAGGGCTTCTGTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAAGAGGGTGGGTCGTAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTc                                                                        <  2:381851/149‑1 (MQ=255)
                                                                             cgcgCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGTTAACCTCCCCAAAAGCGTGCTCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTc                                                                        <  1:37464/149‑1 (MQ=255)
                                                                                  tCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCCCTCCCATa                                                                   >  1:58920/1‑149 (MQ=255)
                                                                                    aaaGAGCTTCTATGATGGTGTGAAGAAGTTTTTTGACGACCTGAGTCGCTAACCTCCGTAACAGCCGTACCTTGGACAGGCCTGGTTGAAAATGCGTTGGGTGTAACACATACGATCTCCTGTAGAGTGACGCTGATCATTCCCATacg                                                                 >  2:58970/1‑147 (MQ=255)
                                                                                          cttcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCATGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGCTATGCGAGCACCTGTACAGTGGCGGGGGTCACTCCCATTAGCGCtca                                                           >  2:657021/1‑147 (MQ=255)
                                                                                                   tGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTACATTCAgggg                                                  >  1:93093/1‑148 (MQ=255)
                                                                                                        tgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg                                             <  1:334667/149‑1 (MQ=255)
                                                                                                                ttttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc                                     <  2:167548/149‑1 (MQ=255)
                                                                                                                      gacgacCTGCCCCGCTAACCTCCCCAAAAGCCGGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                               <  2:108817/149‑1 (MQ=255)
                                                                                                                      gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                               >  2:461690/1‑149 (MQ=255)
                                                                                                                         gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGt                                                                                                                                             >  2:94631/1‑36 (MQ=255)
                                                                                                                         gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGt                                                                                                                                             <  1:94631/36‑1 (MQ=255)
                                                                                                                              gACCCGCTAACCCCCCCAAAAGCCCGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTaa                       <  1:262866/149‑1 (MQ=255)
                                                                                                                                 ccGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATCGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGCTCACTCCCATAAGCGCTAACTTCAGGCTTGTGGTATTACGCCTGATACGATTTAACGt                    >  2:123367/1‑149 (MQ=255)
                                                                                                                                       aCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt              >  2:698420/1‑149 (MQ=255)
                                                                                                                                          tCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgtg                                              <  2:153673/114‑1 (MQ=255)
                                                                                                                                          tCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgtg                                              >  1:153673/1‑114 (MQ=255)
                                                                                                                                           tcaaagaaGTCTGCCGGGCGGAAGGCCAGGGTAAAAATAGGGTGCGTCGAAGCAAGGCGAGCACCTGTAAAGTGGCGGGGGTCACGCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGACTGCTATGCTATAACGTGCAGATGAAt          <  2:222655/143‑1 (MQ=18)
                                                                                                                                             ccAAAAGGCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTa        <  2:570238/149‑1 (MQ=255)
                                                                                                                                                  aGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACtctc   <  2:660987/149‑1 (MQ=255)
                                                                                                                                                   gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa  <  1:698420/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCA  >  NC_000913_3_hsa_tpiA/15163‑15458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.