New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 280699246 (0.960)5 (0.110) 5/284 6.1 10.4% coding (983/1065 nt) proW glycine betaine/proline ABC transporter permease
?NC_000913_3_hsa_tpiA = 2807013 40 (0.840)coding (1004/1065 nt) proW glycine betaine/proline ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGC  >  NC_000913_3_hsa_tpiA/2806865‑2807143
                                                                                                                                                    |                                                                                                                                  
gCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGGCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCgg                                                                                                                                    <  1:510823/149‑1 (MQ=255)
   gccggcggGTTGGGTTCGATGGTGCTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCaa                                                                                                                                 <  2:696879/147‑1 (MQ=255)
         gggTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTTGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGCCCCACGCAGTCGCGGCAACCGTCg                                                                                                                           >  2:334320/1‑149 (MQ=255)
           gTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGCATCACGCAGTCGCGGCAACCGTCGCt                                                                                                                         >  1:514543/1‑149 (MQ=255)
           gTTGGGTCAGATGGTACTTCGAGGTATCGGGCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACAGTCCCt                                                                                                                         >  1:242594/1‑149 (MQ=255)
            ttGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTg                                                                                                                        >  2:647145/1‑149 (MQ=255)
              gggTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAGCCGTCGCTGGt                                                                                                                      >  1:483045/1‑149 (MQ=255)
                ttctgacggTACTTCGCGGTATCGGTCTTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGACCGTCTGACGCAGGCCGTTGGGCCCGCCCTACGCAGTCGCGGCAACCGTCGCTGGCac                                                                                                                    <  2:619783/142‑1 (MQ=255)
                    gATGGTACTTCGCGGTATCGGCCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTAcacca                                                                                                                <  1:27335/149‑1 (MQ=255)
                      tGGTAGTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACt                                                                                                              <  1:567978/149‑1 (MQ=255)
                      tGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACt                                                                                                              <  2:454960/149‑1 (MQ=255)
                        gcaCTCCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGGGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGAAGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTgg                                                                                                            <  1:163767/147‑1 (MQ=255)
                            ttCGCGGTATCGGTCGTCTGGATCTGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCCGGTACACCACTgcgcct                                                                                                        >  1:11851/1‑144 (MQ=255)
                                                 atatGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGACAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCca                                                                                   <  2:615961/149‑1 (MQ=255)
                                                      gggCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcattcat                                                                              >  1:278897/1‑149 (MQ=255)
                                                      gggCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcattcat                                                                              >  1:144347/1‑149 (MQ=255)
                                                       gggttGCCACCGTTGGCGGCGTCGGGATTGTGATCCGCGCCATTAGCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcattcatt                                                                             <  2:458163/146‑1 (MQ=255)
                                                       ggCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcattcatt                                                                             <  1:500461/149‑1 (MQ=255)
                                                          ttGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTCGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAg                                                                          <  2:729656/149‑1 (MQ=255)
                                                                         gcgTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGc                                                           >  2:317165/1‑149 (MQ=255)
                                                                            tCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTGGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAAGTCTGCACTTGCCCg                                                        >  2:19539/1‑149 (MQ=255)
                                                                              gggATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTTCTGACCCGCCCATTCATCAAGTAACTCTGCACTTGCCCGGt                                                      >  2:291486/1‑149 (MQ=255)
                                                                                     tgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCg                                               >  2:340036/1‑149 (MQ=255)
                                                                                          cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCAAGCAATCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTTTGCACTTGCCCGGTGACGCCGGGCAt                                          >  1:581004/1‑149 (MQ=255)
                                                                                              ccATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTCACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATc                                      >  1:611717/1‑149 (MQ=255)
                                                                                               cATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGc                                                                                      >  2:32291/1‑100 (MQ=255)
                                                                                               cATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGc                                                                                      <  1:32291/100‑1 (MQ=255)
                                                                                                aTTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCTGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGATGACGCCGGGCATTATCAc                                    >  1:581887/1‑149 (MQ=255)
                                                                                                       tCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTCAGTAACGCGGCTCTTTCCCGGTGACGCCGGGCATTATCCCCCTGCCa                             >  1:779313/1‑149 (MQ=255)
                                                                                                       tCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCCTTCTCACCCTCCCa                             >  2:261019/1‑149 (MQ=255)
                                                                                                       tCGATCGTCTGACGCAGGCCGTTGAGCAAAACTCACTCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCACTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCa                             >  1:202521/1‑149 (MQ=255)
                                                                                                         gATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCCACCGTCACTGGGACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGAATTATCACCCTGCCaaa                           >  2:350226/1‑149 (MQ=255)
                                                                                                          aTCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaa                          >  1:653789/1‑149 (MQ=255)
                                                                                                             gTCTGACTCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGTCGCCGGGCATTATCACCCTGCCaaaaaaa                       <  1:584160/149‑1 (MQ=255)
                                                                                                             gTCTGACGCAGGGCGTGGGGGCCAACTCACGCAGTAACGGCAACGGTCTGTGGTACACCGCTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaaaa                       <  2:89093/149‑1 (MQ=255)
                                                                                                              gcTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAg                      <  1:168317/148‑1 (MQ=255)
                                                                                                                tGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGa                    <  1:340036/149‑1 (MQ=255)
                                                                                                                     cAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAACAAAAGGAAGAAc               >  1:246566/1‑149 (MQ=255)
                                                                                                                           gTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCg         >  1:711704/1‑149 (MQ=255)
                                                                                                                              gggCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACa      <  1:495588/149‑1 (MQ=255)
                                                                                                                                  gcgACTCACGCAGTCGCGGCAACCGTCGGTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGc  <  2:144347/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                  
GCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGC  >  NC_000913_3_hsa_tpiA/2806865‑2807143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.