Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503–1979270	1979278	9–776	48 [0]	[0] 49	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	48 (0.850)	36/266	0.8	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGC  >  NC_000913_3_hsa_tpiA/1978355‑1978641                                                                                                                                                    |                                                                                                                                            aGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTACCATAAATGt                                                                                                                                            <  2:758994‑M1/149‑2 (MQ=255)   aaaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAGTGCGTGATGCAGATCACACAAAACACTCATTTCCTTATCTTAACTGttt                                                                                                                                          >  1:502305‑M1/1‑146 (MQ=255)      tGGGTCTTTACACTTATCTAAGATTGTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataag                                                                                                                                       <  2:822271‑M1/149‑7 (MQ=255)       ggggCTTTACACTTAACTAAGATTTTTCCTAAATCGACGCAACTGTACTCGACACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagt                                                                                                                                      <  2:167176‑M1/149‑8 (MQ=255)       gggTCTTTACACTTATCTAAGATTTTTCCTAAAGCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagt                                                                                                                                      <  1:122992‑M1/149‑8 (MQ=255)                tactTATCTAAGATTTTTCCAAAATCGACGCAACTGTCTGAGTTACGACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                                                             <  2:896396‑M1/148‑17 (MQ=255)                       cTAAGATTTTTCCTAAATTGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                      <  2:869815‑M1/149‑24 (MQ=255)                       cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                      <  2:119086‑M1/149‑24 (MQ=255)                       cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                      <  1:687217‑M1/149‑24 (MQ=255)                       cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                      <  1:16065‑M1/149‑24 (MQ=255)                        tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                                                                     >  2:408069‑M1/1‑125 (MQ=255)                        tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcata                                                                                                                                  <  1:969884‑M1/136‑12 (MQ=255)                        tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcata                                                                                                                                  >  2:969884‑M1/1‑125 (MQ=255)                                   ccgaggcgacgcaACTGGACTCGTGACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATTtataagtcatacttttgttttgggtgtatttcaat                                                                                                          <  2:677618‑M1/143‑36 (MQ=255)                                   cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                          >  1:309403‑M1/1‑114 (MQ=255)                                   cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                          <  2:309403‑M1/149‑36 (MQ=255)                                    tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc                                                                                                         <  1:346878‑M1/149‑37 (MQ=255)                                        tCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGAGCTGCGATTTTCAATAATGCGAGATGCAGAGCACACAAAACATACAATTACTTAACTTAAATGtataagtactacttttgttatgggtgtattacaaccagtt                                                                                                     >  2:477532‑M1/1‑109 (MQ=255)                                             gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcacacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                <  2:917029‑M1/149‑46 (MQ=255)                                                        cGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcta                                                                                     <  2:751052‑M1/149‑57 (MQ=255)                                                        cGTCACTACACGCACATACAACGGAAGGGGGCTGATATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAAATAAATGtatgagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcta                                                                                     <  2:647616‑M1/149‑57 (MQ=255)                                                         gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctac                                                                                    >  1:265031‑M1/1‑92 (MQ=255)                                                         gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctac                                                                                    >  1:579125‑M1/1‑92 (MQ=255)                                                          tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacg                                                                                   >  1:158444‑M1/1‑91 (MQ=255)                                                                 acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAGAACACTCAATTACTTGACATAAATGtataaagcatacttttgttttggttgtgtttcaatctgttacaaagtctgtcgctacgctagcaa                                                                            >  2:723614‑M1/1‑84 (MQ=255)                                                                           aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgct                                                                                 <  2:134887‑M1/134‑61 (MQ=255)                                                                           aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgct                                                                                 >  1:134887‑M1/1‑74 (MQ=255)                                                                            aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctggcaagcaaaaatgaa                                                                 >  1:380320‑M1/1‑73 (MQ=255)                                                                            aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaa                                                                 >  1:61837‑M1/1‑73 (MQ=255)                                                                             cGGAGGGGTGCTGCGATTTTCAATAATGCATGATGCAGATCACATAAAACACTCAATTACTTAACATAAATGtataagtcatacttctgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                <  1:269783‑M1/149‑78 (MQ=255)                                                                                        tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttagatagtttttcgctacgctagcaagcaaaaatgtaacgggaataata                                                     >  2:899779‑M1/1‑61 (MQ=255)                                                                                        tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                     >  2:412487‑M1/1‑61 (MQ=255)                                                                                               ttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgg                                              <  2:265031‑M1/149‑96 (MQ=255)                                                                                                tCAATGATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaactggaataatcgaaatggg                                             <  2:711608‑M1/149‑97 (MQ=255)                                                                                                  aataatGCGTGATGCAGATCACCCAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtatatttcaatctgttaaaaagtttttcgataggctcgcaaggaatcattaaacacgaataatctaaatgggtg                                           >  2:907854‑M1/1‑51 (MQ=255)                                                                                                  aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                           >  2:459216‑M1/1‑51 (MQ=255)                                                                                                  aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                           >  2:896739‑M1/1‑51 (MQ=255)                                                                                                             aTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgctaaaaagtttttcgccaagtctcagtgcagaaacgaaaccggaataatcgaaatgggatgttgcgcacag                                >  1:766118‑M1/1‑40 (MQ=255)                                                                                                               gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatagaaatgggattttgcgcacagtc                              >  2:268432‑M1/1‑38 (MQ=255)                                                                                                                  gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacaatcaaa                           >  1:526890‑M1/1‑35 (MQ=255)                                                                                                                      acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                       <  1:740189‑M1/149‑119 (MQ=255)                                                                                                                       cacaAAACACTCAATTACTTAACAAAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataac                      <  2:502305‑M1/149‑120 (MQ=255)                                                                                                                        acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgatacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataact                     >  1:837623‑M1/1‑29 (MQ=255)                                                                                                                          aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaacatgaaacaggaataatcgaaatgggctgttgcgcacagtcaaaatcactta                   >  1:363381‑M1/1‑27 (MQ=255)                                                                                                                            aaCACTCCAGTACTTAACATAAACGtatatgtcatactttggcttggggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcacc                 <  1:219438‑M1/149‑125 (MQ=255)                                                                                                                                        cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcat     <  2:750192‑M1/149‑137 (MQ=255)                                                                                                                                         ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc    <  1:408069‑M1/149‑138 (MQ=255)                                                                                                                                           tacATAAATGtataagtcctacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctg  <  1:1004911‑M1/148‑140 (MQ=255)                                                                                                                                                    |                                                                                                                                            AGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGC  >  NC_000913_3_hsa_tpiA/1978355‑1978641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑