Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503–1979270	1979278	9–776	48 [0]	[0] 49	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	48 (0.850)	36/266	0.8	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAA  >  NC_000913_3_hsa_tpiA/1979131‑1979427                                                                                                                                                     |                                                                                                                                                      agaaatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaccataaatgt                                                                                                                                                       <  2:758994‑M2/1‑1 (MQ=255)   aaatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaatagtgcgtgatgcagatcacacaaaacactcatttccttatcttaactgttt                                                                                                                                                     >  1:502305‑M2/147‑147 (MQ=255)      tgggtctttacacttatctaagattgttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAg                                                                                                                                                  <  2:822271‑M2/6‑1 (MQ=255)       gggtctttacacttatctaagatttttcctaaagcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGt                                                                                                                                                 <  1:122992‑M2/7‑1 (MQ=255)       ggggctttacacttaactaagatttttcctaaatcgacgcaactgtactcgacactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGt                                                                                                                                                 <  2:167176‑M2/7‑1 (MQ=255)                tacttatctaagatttttccaaaatcgacgcaactgtctgagttacgacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACtttt                                                                                                                                        <  2:896396‑M2/16‑1 (MQ=255)                       ctaagatttttcctaaattgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                                 <  2:869815‑M2/23‑1 (MQ=255)                       ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                                 <  1:16065‑M2/23‑1 (MQ=255)                       ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                                 <  2:119086‑M2/23‑1 (MQ=255)                       ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                                 <  1:687217‑M2/23‑1 (MQ=255)                        taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATa                                                                                                                                             >  2:969884‑M2/126‑136 (MQ=255)                        taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATa                                                                                                                                             <  1:969884‑M2/11‑1 (MQ=255)                        taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                                >  2:408069‑M2/126‑149 (MQ=255)                                   ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                     <  2:309403‑M2/35‑1 (MQ=255)                                   ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                     >  1:309403‑M2/115‑149 (MQ=255)                                   ccgaggcgacgcaactggactcgtgactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaattTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                     <  2:677618‑M2/35‑1 (MQ=255)                                    taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                                                                    <  1:346878‑M2/36‑1 (MQ=255)                                        tcgacgcaactgtactcgtcactacacgcacatacaacggaggggagctgcgattttcaataatgcgagatgcagagcacacaaaacatacaattacttaacttaaatgTATAAGTACTACTTTTGTTATGGGTGTATTACAACCAGtt                                                                                                                >  2:477532‑M2/110‑149 (MQ=255)                                             gcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCACACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaaa                                                                                                           <  2:917029‑M2/45‑1 (MQ=255)                                                        cgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgcta                                                                                                <  2:751052‑M2/56‑1 (MQ=255)                                                        cgtcactacacgcacatacaacggaagggggctgatattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaaaataaatgTATGAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgcta                                                                                                <  2:647616‑M2/56‑1 (MQ=255)                                                         gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctac                                                                                               >  1:265031‑M2/93‑149 (MQ=255)                                                         gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctac                                                                                               >  1:579125‑M2/93‑149 (MQ=255)                                                          tcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacg                                                                                              >  1:158444‑M2/92‑149 (MQ=255)                                                                 acgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacagaacactcaattacttgacataaatgTATAAAGCATACTTTTGTTTTGGTTGTGTTTCAATCTGTTACAAAGTCTGTCGCTACGCTagca‑a                                                                                      >  2:723614‑M2/85‑149 (MQ=255)                                                                           aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgct                                                                                            >  1:134887‑M2/75‑134 (MQ=255)                                                                           aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgct                                                                                            <  2:134887‑M2/60‑1 (MQ=255)                                                                            acggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTGGCA‑AGCAAAAATGaa                                                                           >  1:380320‑M2/74‑149 (MQ=255)                                                                            acggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGaa                                                                           >  1:61837‑M2/74‑149 (MQ=255)                                                                             cggaggggtgctgcgattttcaataatgcatgatgcagatcacataaaacactcaattacttaacataaatgTATAAGTCATACTTCTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGaaa                                                                          <  1:269783‑M2/77‑1 (MQ=255)                                                                                        tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAGATAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGTAACGGgaataata                                                               >  2:899779‑M2/62‑148 (MQ=255)                                                                                        tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATc                                                               >  2:412487‑M2/62‑149 (MQ=255)                                                                                               ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATgg                                                        <  2:265031‑M2/95‑1 (MQ=255)                                                                                                tcaatgatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACTGGAATAATCGAAATggg                                                       <  2:711608‑M2/96‑1 (MQ=255)                                                                                                  aataatgcgtgatgcagatcacccaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTATATTTCAATCTGTTAAAAAGTTTTTCGATAGGCTCGCA‑AGGAATCATTAAACACGAATAATCTAAatgggtg                                                     >  2:907854‑M2/52‑147 (MQ=255)                                                                                                  aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGAt                                                     >  2:459216‑M2/52‑149 (MQ=255)                                                                                                  aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGAt                                                     >  2:896739‑M2/52‑149 (MQ=255)                                                                                                             atgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGCTAAAAAGTTTTTCGCCAAG‑TCTCAGTGCAGAAACGAAACCGGAATAATCGAAATGGGATGTTGCGCACAg                                          >  1:766118‑M2/41‑149 (MQ=255)                                                                                                               gcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATAGAAATGGGATTTTGCGCACAGTc                                        >  2:268432‑M2/39‑149 (MQ=255)                                                                                                                  gatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAATCaaa                                     >  1:526890‑M2/36‑149 (MQ=255)                                                                                                                      acacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATaa                                 <  1:740189‑M2/118‑1 (MQ=255)                                                                                                                       cacaaaacactcaattacttaacaaaaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAAc                                <  2:502305‑M2/119‑1 (MQ=255)                                                                                                                        acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGATACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACt                               >  1:837623‑M2/30‑149 (MQ=255)                                                                                                                          aaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAACATGAAACAGGAATAATCGAAATGGGCTGTTGCGCACAGTCAAAATCactta                             >  1:363381‑M2/28‑147 (MQ=255)                                                                                                                            aacactccagtacttaacataaacgTATATGTCATACTTTGGCTTGGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCAcc                           <  1:219438‑M2/124‑1 (MQ=255)                                                                                                                                        cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcat               <  2:750192‑M2/136‑1 (MQ=255)                                                                                                                                         ttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcatc              <  1:408069‑M2/137‑1 (MQ=255)                                                                                                                                           tacataaatgTATAAGTCCTACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTg            <  1:1004911‑M2/139‑1 (MQ=255)                                                                                                                                                     tataAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATTTGCTataaataa  >  1:427075/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCA‑AGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAA  >  NC_000913_3_hsa_tpiA/1979131‑1979427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑