New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	2544028 =	54 (1.380)	4 (0.110)	3/270	5.5	7.2%	coding (596/792 nt)	ucpA	furfural resistance protein, putative short‑chain oxidoreductase
	?	NC_000913_3_hsa_tpiA	2544050 =	52 (1.390)					coding (574/792 nt)	ucpA	furfural resistance protein, putative short‑chain oxidoreductase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCA  >  NC_000913_3_hsa_tpiA/2543882‑2544174                                                                                                                                                   |                                                                                                                                                   caTTCTTTGGACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGc                                                                                                                                                 <  2:481434/150‑1 (MQ=255) caTTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGc                                                                                                                                                 <  1:458243/150‑1 (MQ=255)  aTTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCg                                                                                                                                                <  1:350789/150‑1 (MQ=255)       gtgtACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCaa                                                                                                                                           <  2:396400/150‑1 (MQ=255)       gtgtACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGAATCTTCCGGGTTCGACTGGCCGGCaa                                                                                                                                           >  2:239991/1‑150 (MQ=255)            ccGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCtt                                                                                                                                      >  2:561158/1‑150 (MQ=255)             cGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCttt                                                                                                                                     >  2:489357/1‑150 (MQ=255)                               cATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCa                                                                                                                   >  1:513544/1‑150 (MQ=255)                                 tCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGCCGCATCGGCATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGTGTTCGACTTGCGGGCAATGCTTTCCGCCATTGGTGTGCGCAcc                                                                                                                 >  2:576990/1‑149 (MQ=255)                                    gATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTAt                                                                                                              <  2:513544/150‑1 (MQ=255)                                         gAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGAGGGCAATGGTTACCTCCATGGGTGTGCGCACGTATccccg                                                                                                         >  2:316538/1‑148 (MQ=255)                                               ggCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAAt                                                                                                   >  2:22667/1‑150 (MQ=255)                                                  cgccAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGc                                                                                                <  1:218786/150‑1 (MQ=255)                                                  cgccAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGAATCGAGTTTGCTTTCGCCATTTCATTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGc                                                                                                <  2:490505/150‑1 (MQ=255)                                                     cAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTGCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAAGGCTTTCCGCCATTCGTGTGCGCACGTATACCGAGCGAATGGCGtt                                                                                             >  2:292411/1‑150 (MQ=255)                                                          cGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAAcgc                                                                                        <  1:489357/150‑1 (MQ=255)                                                          cGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAAcgc                                                                                        >  1:684084/1‑150 (MQ=255)                                                            ccGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCCCGTATCCCGGGCAAATGGCGTTAACGCGa                                                                                      >  1:24657/1‑150 (MQ=255)                                                              gACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAAt                                                                                    <  1:42865/150‑1 (MQ=255)                                                               acctCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCCGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATa                                                                                   <  1:46556/147‑1 (MQ=255)                                                                  tCCAGCGGATCGGCGAGGCGACGCATCGGGGTTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATTTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCa                                                                                <  2:128358/150‑1 (MQ=255)                                                                  tCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCa                                                                                <  1:439801/150‑1 (MQ=255)                                                                    cAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTGGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGa                                                                              <  2:136167/150‑1 (MQ=255)                                                                         gATCGGCGAGGCGACGCATCGGGATTGCGTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGGTCGGCTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATAGCGTTAACGCGAATACCGGGCTgcc                                                                         >  1:138058/1‑149 (MQ=255)                                                                          aTCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTgcgc                                                                        <  2:376516/150‑1 (MQ=255)                                                                           tCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCGGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTcc                                                                                                                                   >  2:651663/1‑90 (MQ=255)                                                                           tCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTcc                                                                                                                                   <  1:651663/90‑1 (MQ=255)                                                                                   gcgaCGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCAc                                                               >  1:485544/1‑150 (MQ=255)                                                                                      aCGCATCGGGATTGCTTTCGCCATTGCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGc                                                            <  2:141981/150‑1 (MQ=255)                                                                                                actGCTCTCGCCCTTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGAtttt                                                  <  1:25062/148‑1 (MQ=255)                                                                                                    cTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCa                                              <  2:610391/150‑1 (MQ=255)                                                                                                     tttCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGGTAACGCGAATACCAGACTGCGCGTACTCCACCGCCCGCGACTTTGTCAg                                             >  2:160710/1‑150 (MQ=255)                                                                                                         gCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCca                                         >  2:687615/1‑150 (MQ=255)                                                                                                           cATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGc                                                            >  2:234860/1‑129 (MQ=255)                                                                                                           cATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGc                                                            <  1:234860/129‑1 (MQ=255)                                                                                                            aTTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCcaaca                                      <  2:256945/150‑1 (MQ=255)                                                                                                                  gtcagCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTAc                                                                    <  2:51561/114‑1 (MQ=255)                                                                                                                  gtcagCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTAc                                                                    >  1:51561/1‑114 (MQ=255)                                                                                                                  gtcagCACCGACTCTGGATCTTCCGGGTTAGAATGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATcgcc                                >  2:310309/1‑150 (MQ=255)                                                                                                                   tcagCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATcgccg                               <  2:396730/150‑1 (MQ=255)                                                                                                                       cACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCttt                           >  1:633517/1‑150 (MQ=255)                                                                                                                       cACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGATAACGCGAATCCCAGACTGCGCGTACTCCACCGCCAGCGATTTTGCAAGGCCAACAATCGCCGCttt                           >  2:110341/1‑150 (MQ=255)                                                                                                                           gACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGtt                       >  2:100945/1‑150 (MQ=255)                                                                                                                              tctGGATCTTCCGGTTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAg                    <  1:310309/150‑1 (MQ=255)                                                                                                                               ctGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAgg                   >  1:464589/1‑150 (MQ=255)                                                                                                                                tGGATCTTCCGGGTCCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGc                  >  2:82157/1‑150 (MQ=255)                                                                                                                                    tCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTAc              >  2:37678/1‑150 (MQ=255)                                                                                                                                         cGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGt         >  1:479988/1‑150 (MQ=255)                                                                                                                                           ggTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGttt       >  2:585184/1‑150 (MQ=255)                                                                                                                                              tCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGc    <  1:22667/150‑1 (MQ=255)                                                                                                                                                gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa  <  1:84848/150‑1 (MQ=255)                                                                                                                                                gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa  >  2:642966/1‑150 (MQ=255)                                                                                                                                                gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa  <  1:37678/150‑1 (MQ=255)                                                                                                                                                gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa  >  1:205580/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCA  >  NC_000913_3_hsa_tpiA/2543882‑2544174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.