New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	2544028 =	54 (1.380)	4 (0.110)	3/270	5.5	7.2%	coding (596/792 nt)	ucpA	furfural resistance protein, putative short‑chain oxidoreductase
	?	NC_000913_3_hsa_tpiA	2544050 =	52 (1.390)					coding (574/792 nt)	ucpA	furfural resistance protein, putative short‑chain oxidoreductase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCA‑TATCACCA  >  NC_000913_3_hsa_tpiA/2543912‑2544195                                                                                                                                           |                                                                                                                                                   cATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCa                                                                                                                                         >  1:513544/1‑150 (MQ=255)   tCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGCCGCATCGGCATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGTGTTCGACTTGCGGGCAATGCTTTCCGCCATTGGTGTGCGCAcc                                                                                                                                       >  2:576990/1‑149 (MQ=255)      gATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTAt                                                                                                                                    <  2:513544/150‑1 (MQ=255)           gAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGAGGGCAATGGTTACCTCCATGGGTGTGCGCACGTATccccg                                                                                                                               >  2:316538/1‑148 (MQ=255)                 ggCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAAt                                                                                                                         >  2:22667/1‑150 (MQ=255)                    cgccAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGc                                                                                                                      <  1:218786/150‑1 (MQ=255)                    cgccAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGAATCGAGTTTGCTTTCGCCATTTCATTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGc                                                                                                                      <  2:490505/150‑1 (MQ=255)                       cAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTGCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAAGGCTTTCCGCCATTCGTGTGCGCACGTATACCGAGCGAATGGCGtt                                                                                                                   >  2:292411/1‑150 (MQ=255)                            cGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAAcgc                                                                                                              >  1:684084/1‑150 (MQ=255)                            cGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAAcgc                                                                                                              <  1:489357/150‑1 (MQ=255)                              ccGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCCCGTATCCCGGGCAAATGGCGTTAACGCGa                                                                                                            >  1:24657/1‑150 (MQ=255)                                gACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAAt                                                                                                          <  1:42865/150‑1 (MQ=255)                                 acctCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCCGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATa                                                                                                         <  1:46556/147‑1 (MQ=255)                                    tCCAGCGGATCGGCGAGGCGACGCATCGGGGTTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATTTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCa                                                                                                      <  2:128358/150‑1 (MQ=255)                                    tCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCa                                                                                                      <  1:439801/150‑1 (MQ=255)                                      cAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTGGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGa                                                                                                    <  2:136167/150‑1 (MQ=255)                                           gATCGGCGAGGCGACGCATCGGGATTGCGTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGGTCGGCTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATAGCGTTAACGCGAATACCGGGCTgcc                                                                                               >  1:138058/1‑149 (MQ=255)                                            aTCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTgcgc                                                                                              <  2:376516/150‑1 (MQ=255)                                                     gcgaCGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCAc                                                                                     >  1:485544/1‑150 (MQ=255)                                                        aCGCATCGGGATTGCTTTCGCCATTGCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGc                                                                                  <  2:141981/150‑1 (MQ=255)                                                                  actGCTCTCGCCCTTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGAtttt                                                                        <  1:25062/148‑1 (MQ=255)                                                                      cTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCa                                                                    <  2:610391/150‑1 (MQ=255)                                                                       tttCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGGTAACGCGAATACCAGACTGCGCGTACTCCACCGCCCGCGACTTTGTCAg                                                                   >  2:160710/1‑150 (MQ=255)                                                                           gCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCca                                                               >  2:687615/1‑150 (MQ=255)                                                                             cATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGc                                                                                  >  2:234860/1‑129 (MQ=255)                                                                             cATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGc                                                                                  <  1:234860/129‑1 (MQ=255)                                                                              aTTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCcaaca                                                            <  2:256945/150‑1 (MQ=255)                                                                                    gtcagCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTAc                                                                                          <  2:51561/114‑1 (MQ=255)                                                                                    gtcagCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTAc                                                                                          >  1:51561/1‑114 (MQ=255)                                                                                    gtcagCACCGACTCTGGATCTTCCGGGTTAGAATGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATcgcc                                                      >  2:310309/1‑150 (MQ=255)                                                                                     tcagCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATcgccg                                                     <  2:396730/150‑1 (MQ=255)                                                                                         cACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCttt                                                 >  1:633517/1‑150 (MQ=255)                                                                                         cACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGATAACGCGAATCCCAGACTGCGCGTACTCCACCGCCAGCGATTTTGCAAGGCCAACAATCGCCGCttt                                                 >  2:110341/1‑150 (MQ=255)                                                                                             gACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGtt                                             >  2:100945/1‑150 (MQ=255)                                                                                                tctGGATCTTCCGGTTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAg                                          <  1:310309/150‑1 (MQ=255)                                                                                                 ctGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAgg                                         >  1:464589/1‑150 (MQ=255)                                                                                                  tGGATCTTCCGGGTCCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGc                                        >  2:82157/1‑150 (MQ=255)                                                                                                      tCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTAc                                    >  2:37678/1‑150 (MQ=255)                                                                                                           cGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGt                               >  1:479988/1‑150 (MQ=255)                                                                                                             ggTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGttt                             >  2:585184/1‑150 (MQ=255)                                                                                                                tCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGc                          <  1:22667/150‑1 (MQ=255)                                                                                                                  gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa                        >  2:642966/1‑150 (MQ=255)                                                                                                                  gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa                        >  1:205580/1‑150 (MQ=255)                                                                                                                  gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa                        <  1:84848/150‑1 (MQ=255)                                                                                                                  gACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCa                        <  1:37678/150‑1 (MQ=255)                                                                                                                       gCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATc                   <  1:561158/150‑1 (MQ=255)                                                                                                                          ggCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGc                <  2:684084/150‑1 (MQ=255)                                                                                                                             aaTGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGccac             <  2:551940/150‑1 (MQ=255)                                                                                                                               tGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGccacca           <  2:276891/150‑1 (MQ=255)                                                                                                                                  tttCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCa‑tat       >  2:564429/1‑150 (MQ=255)                                                                                                                                  tttCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCa‑tat       >  2:219078/1‑150 (MQ=255)                                                                                                                                   ttCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATTGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCA‑TATc      >  2:432156/1‑150 (MQ=255)                                                                                                                                     ccGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTCCGCCGTTTCGCCAGGATC‑GCCACCACTATCAc    >  2:157119/1‑150 (MQ=255)                                                                                                                                       gCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCA‑TCTCACCa  >  2:469819/1‑150 (MQ=255)                                                                                                                                           |                                                                                                                                                   CATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGCGAGGCGACGCATCGGGATTGCTTTCGCCATTTCAGTCAGCACCGACTCTGGATCTTCCGGGTTCGACTGGCGGGCAATGCTTTCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCA‑TATCACCA  >  NC_000913_3_hsa_tpiA/2543912‑2544195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.