New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 321348019 (0.800)4 (0.180) 4/272 2.7 16.3% coding (434/1842 nt) rpoD RNA polymerase, sigma 70 (sigma D) factor
?NC_000913_3_hsa_tpiA = 3213501 23 (1.010)coding (455/1842 nt) rpoD RNA polymerase, sigma 70 (sigma D) factor
Rejected: Frequency below/above cutoff threshold.

GAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACG  >  NC_000913_3_hsa_tpiA/3213356‑3213626
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gAAAGGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAagc                                                                                                                            >  1:21482‑M2/1‑146 (MQ=255)
    tGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAGTGAAGACGGGATCTACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCagaa                                                                                                                        <  1:335917/149‑1 (MQ=255)
           cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAgcgc                                                                                                                 >  1:284866/1‑149 (MQ=255)
            gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGcgcg                                                                                                                >  1:28920/1‑149 (MQ=255)
                   tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTtcaacacgatcgtactgttccagcagatagg                                                                                                                     <  2:288552‑M1/137‑32 (MQ=255)
                   tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTtcaacacgatcgtactgttccagcagatagg                                                                                                                     >  1:288552‑M1/1‑106 (MQ=255)
                            gcgAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATc                                                                                                >  1:179548/1‑149 (MQ=255)
                              gAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCAc                                                                                              <  1:253151/149‑1 (MQ=255)
                               aaGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGGTGAAGCAGAAGAAGCGGGTCTGTCCGATCTGATCAcc                                                                                             <  1:416395/149‑1 (MQ=255)
                                             aTCGCTAAGCGTATTGACGACGGGATCAACCAGGTTCAATACTCCGTCGCTGAATATCCGGAAGCGATCACCTATCTGCTGCAACAGTACGATCGTGTTGCAGCAGCAAAAGCGCGTCTGTCCGATCTGATCACCGGCTCTGATGAccc                                                                               >  2:363333/1‑149 (MQ=255)
                                             aTCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgac                                                                                 <  1:367266‑M2/147‑47 (MQ=255)
                                             aTCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgaa                                                                                 >  2:367266‑M2/1‑101 (MQ=255)
                                                gCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGaa                                                                            >  1:249523/1‑149 (MQ=255)
                                                 cTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATAGCGTTGAAGCACAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAAc                                                                           >  1:222589/1‑149 (MQ=255)
                                                                  gggATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTCATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGc                                                          >  2:322469/1‑149 (MQ=255)
                                                                     aTCAACCAGGTTCAATGCTCCGCTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCAcc                                                       <  1:96692/149‑1 (MQ=255)
                                                                     aTCAAACAGGTTAAAGGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCAcc                                                       <  1:33856/149‑1 (MQ=255)
                                                                         aCCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTAcc                                                   <  1:403119/149‑1 (MQ=255)
                                                                              gtacAATGCTCCGTTGCTGAATATCCGGAAGCGAGCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGATGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCAc                                              <  2:84128/146‑1 (MQ=255)
                                                                               ttCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTtcaacacgatcgtactgttccagcagataggtgatcgcttccggatattcagcaacggagcattgaacctggttgatcccgtcttcaatacgcttagcgat                                               >  2:367266‑M1/1‑46 (MQ=255)
                                                                               gtcAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTtcaacacgatcgtactgttccagcagataggtgatcgcttccggatattcagcaacggagcattgaacctggttgatcccgtcttcaatacgcttagcgat                                               <  1:367266‑M1/146‑102 (MQ=255)
                                                                                              cTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTCATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAg                              <  1:319045/149‑1 (MQ=255)
                                                                                               tGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGAGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGCCACCTACCGCCACTCACGCAGGTTCTGAGc                             >  2:192652/1‑149 (MQ=255)
                                                                                                                 cACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTgg           <  2:284866/149‑1 (MQ=255)
                                                                                                                   ccTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgaacctggttgatcccgtcttcaatacgcttagcgatgtcaatttcgccttcgcgggtcaaca                     >  1:288552‑M2/1‑31 (MQ=255)
                                                                                                                   ccTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgaacctggttgatcccgtcttcaatacgcttagcgatgtcaatttcgccttcgcgggtcaaca                     <  2:288552‑M2/137‑107 (MQ=255)
                                                                                                                     tATCGGCTGGAACAGGACGATCGTGTTGAAGCTGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGa       <  2:98526/149‑1 (MQ=255)
                                                                                                                      aTCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGAt      >  2:322361/1‑149 (MQ=255)
                                                                                                                          gCTtcaacacgatcgtactgttccagcagataggtgatcgcttccggatattcagcaacggagcattgaacctggttgatcccgtcttcaatacgcttagcgatgtcaatttcgccttcgcgggtcaacagttcaacggtgcccctttc  >  1:21482‑M1/1‑3 (MQ=255)
                                                                                                                            |                                                                                                                                                  
GAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACG  >  NC_000913_3_hsa_tpiA/3213356‑3213626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.