New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 3213480	19 (0.800)	4 (0.180)	4/272	2.7	16.3%	coding (434/1842 nt)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
	?	NC_000913_3_hsa_tpiA	= 3213501	23 (1.010)					coding (455/1842 nt)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Rejected: Frequency below/above cutoff threshold.

GAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGAT  >  NC_000913_3_hsa_tpiA/3213356‑3213646                                                                                                                                                  |                                                                                                                                                  gAAAGGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAagc                                                                                                                                                >  1:21482‑M2/1‑146 (MQ=255)     tGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAGTGAAGACGGGATCTACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCagaa                                                                                                                                            <  1:335917/149‑1 (MQ=255)            cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAgcgc                                                                                                                                     >  1:284866/1‑149 (MQ=255)             gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGcgcg                                                                                                                                    >  1:28920/1‑149 (MQ=255)                             gcgAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATc                                                                                                                    >  1:179548/1‑149 (MQ=255)                               gAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCAc                                                                                                                  <  1:253151/149‑1 (MQ=255)                                aaGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGGTGAAGCAGAAGAAGCGGGTCTGTCCGATCTGATCAcc                                                                                                                 <  1:416395/149‑1 (MQ=255)                                              aTCGCTAAGCGTATTGACGACGGGATCAACCAGGTTCAATACTCCGTCGCTGAATATCCGGAAGCGATCACCTATCTGCTGCAACAGTACGATCGTGTTGCAGCAGCAAAAGCGCGTCTGTCCGATCTGATCACCGGCTCTGATGAccc                                                                                                   >  2:363333/1‑149 (MQ=255)                                              aTCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgac                                                                                                     <  1:367266‑M2/147‑47 (MQ=255)                                              aTCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgaa                                                                                                     >  2:367266‑M2/1‑101 (MQ=255)                                                 gCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGaa                                                                                                >  1:249523/1‑149 (MQ=255)                                                  cTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATAGCGTTGAAGCACAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAAc                                                                                               >  1:222589/1‑149 (MQ=255)                                                                   gggATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTCATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGc                                                                              >  2:322469/1‑149 (MQ=255)                                                                      aTCAACCAGGTTCAATGCTCCGCTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCAcc                                                                           <  1:96692/149‑1 (MQ=255)                                                                      aTCAAACAGGTTAAAGGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCAcc                                                                           <  1:33856/149‑1 (MQ=255)                                                                          aCCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTAcc                                                                       <  1:403119/149‑1 (MQ=255)                                                                               gtacAATGCTCCGTTGCTGAATATCCGGAAGCGAGCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGATGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCAc                                                                  <  2:84128/146‑1 (MQ=255)                                                                                               cTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTCATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAg                                                  <  1:319045/149‑1 (MQ=255)                                                                                                tGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGAGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGCCACCTACCGCCACTCACGCAGGTTCTGAGc                                                 >  2:192652/1‑149 (MQ=255)                                                                                                                  cACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTgg                               <  2:284866/149‑1 (MQ=255)                                                                                                                    ccTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgaacctggttgatcccgtcttcaatacgcttagcgatgtcaatttcgccttcgcgggtcaaca                                         >  1:288552‑M2/1‑31 (MQ=255)                                                                                                                    ccTATCTGCTGGAACAGTACGATCGTGTTGAagcagataggtgatcgcttccggatattcagcaacggagcattgaacctggttgatcccgtcttcaatacgcttagcgatgtcaatttcgccttcgcgggtcaaca                                         <  2:288552‑M2/137‑107 (MQ=255)                                                                                                                      tATCGGCTGGAACAGGACGATCGTGTTGAAGCTGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGa                           <  2:98526/149‑1 (MQ=255)                                                                                                                       aTCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGAt                          >  2:322361/1‑149 (MQ=255)                                                                                                                                     tACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGa            >  1:130243/1‑149 (MQ=255)                                                                                                                                        gATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACga         >  2:337704/1‑149 (MQ=255)                                                                                                                                        gATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACga         >  1:46450/1‑149 (MQ=255)                                                                                                                                               ttGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGCAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGAt  >  1:129907/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGAT  >  NC_000913_3_hsa_tpiA/3213356‑3213646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.