New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 3827430 =19 (0.800)4 (0.180) 4/270 2.7 17.0% intergenic (+139/+30) recG/gltS ATP‑dependent DNA helicase/glutamate transporter
?NC_000913_3_hsa_tpiA 3827452 = 21 (0.930)intergenic (+161/+8) recG/gltS ATP‑dependent DNA helicase/glutamate transporter
Rejected: Frequency below/above cutoff threshold.

AGACCGCCATTTCAGGTTACCCTACCTTCCTGCCGGATGCGATTCATCACCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCG  >  NC_000913_3_hsa_tpiA/3827307‑3827590
                                                                                                                                                 |                                                                                                                                           
tgggccgccgcccttggtgtttttggtggtgccgatggtcggtgcgttctttatcgatatcgtcaatgcgctggtcattatgttgtatttggtgttgccgattcttgccggttaaccgatgaaGCGGCGGCACTTCTACCGCCGCTTCa                                                                                                                                          >  2:239930‑M1/124‑149 (MQ=255)
   ccGCCATTTCAGGTTACCCTACCTTCCTGCCCCATGCGATTCATCACCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGGGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCg                                                                                                                                       >  1:176840/1‑149 (MQ=255)
                                             atcaCCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCgg                                                                                                                    <  2:345282/126‑1 (MQ=255)
                                             atcaCCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCgg                                                                                                                    >  1:345282/1‑126 (MQ=255)
                                             atcaCCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGCCAACATCAAATACAACTTAATTa                                                                                             >  2:298434/1‑149 (MQ=255)
                                              tcaCCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTAc                                                                                            >  2:114051/1‑149 (MQ=255)
                                              tcaCCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTAc                                                                                            >  2:114747/1‑149 (MQ=255)
                                                  ccTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAgc                                                                                        <  1:240576/149‑1 (MQ=255)
                                                            aaaTAAATTATGAATCAATACGCAGGCTTGCGAACCGACGCCTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGa                                                                              <  1:326004/148‑1 (MQ=255)
                                                                    tATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCACAAATCGGCCACATCAAATACAACTTAATTCCCAGCGCGTTGCCGATATCGagt                                                                      >  2:278486/1‑147 (MQ=255)
                                                                        aaTCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGa                                                                  >  1:235064/1‑149 (MQ=255)
                                                                            attaagttgtatttgatgttgccgatttttgccggttaaccgatgaaGCGGCGGCACTTCTACCGCCGCtt                                                                                                                                            <  2:220011‑M1/24‑1 (MQ=255)
                                                                            attaagttgtatttgatgttgccgatttttgccggttaaccgatgaaGCGGCGGCACTTCTACCGCCGCtt                                                                                                                                            >  1:220011‑M1/48‑71 (MQ=255)
                                                                                   aGGCTTGATAAGCGAAGCGAATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAACAAACGgaccgacc                                                       >  2:69193/1‑149 (MQ=255)
                                                                                              gcgaagcgTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCCAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGCCCATCGGcacccc                                            >  2:202245/1‑147 (MQ=255)
                                                                                                 aagcgTATCAGGCATTTGTCTTTGTTGCGGCGGCGCTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCaa                                        >  2:315301/1‑149 (MQ=255)
                                                                                                            gCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCAtgt                             >  2:278932/1‑149 (MQ=255)
                                                                                                              aTTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGAACCGACCATCGGCACCACCAAAGACGCAATCTGc                           >  2:395113/1‑149 (MQ=255)
                                                                                                                  gTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACg                       >  1:419302/1‑149 (MQ=255)
                                                                                                                     tttGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCCCCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGc                    >  1:30438/1‑149 (MQ=255)
                                                                                                                       tgaagcggcggtagaagtgccgccgcTTCATCGGTTAACCGGCAAGAATCGGCAACACCAAATACAACATAATGACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCAAGGGCGGCGGCCCa                  >  2:239930‑M2/27‑149 (MQ=255)
                                                                                                                         aagcggcggtagaagtgccgccgcTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAAt                                                                                               <  2:220011‑M2/47‑1 (MQ=255)
                                                                                                                         aagcggcggtagaagtgccgccgcTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAAt                                                                                               >  1:220011‑M2/25‑71 (MQ=255)
                                                                                                                           gcggcggtagaagtgccgccgcTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTACGACGGGCCAAAGc              >  2:340204‑M2/23‑149 (MQ=255)
                                                                                                                                    cTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGa     <  1:114747/149‑1 (MQ=255)
                                                                                                                                    cTTCGACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGa     <  1:114051/149‑1 (MQ=255)
                                                                                                                                       cTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCGCCCAAACCGCCATGTGCGACCGGGCAACGCGCTCAGTCATCg  >  1:138911/1‑149 (MQ=255)
                                                                                                                                       cTACCGCCGCATCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATAACCAGCGCATTGACGCTATCGATAAAGAAAGCACCCACCATCGGCACCACACAAAAACCAATGTACGACGGACCAAAGCGGTCAGtagtag  >  2:76212/1‑144 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
AGACCGCCATTTCAGGTTACCCTACCTTCCTGCCGGATGCGATTCATCACCCTACAAATTCAATAAATTATGAATCAATACGCAGGCTTGATAAGCGAAGCGTATCAGGCATTTGTCTTTGTTGCGGCGGCACTTCTACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCG  >  NC_000913_3_hsa_tpiA/3827307‑3827590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.