New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 166522519 (0.800)4 (0.180) 4/268 2.6 17.5% intergenic (+105/‑90) dmsD/clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
?NC_000913_3_hsa_tpiA = 1665236 20 (0.900)intergenic (+116/‑79) dmsD/clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
Rejected: Frequency below/above cutoff threshold.

AATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/1665074‑1665225
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttttgcggcAAAAGAGGCCAGCCGCAAAAGCAGTGCGTGCTTTAAGAGAT  <  NC_000913_3_hsa_tpiA/1665236‑1665196
                                                                                                                                                                                                 
AATCACAACTTGTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGC                                              <  2:343743/149‑1
                                                                 GGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCAAAAGAG                                    >  1:250003/1‑94
                                                                 GGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCAAAAGAG                                    <  2:250003/94‑1
                                                                                           GCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCAAAAGAGGCCAGCCGCAAAAGCAGTGCGTGCTTTAAGAGAT  >  1:167340/1‑102
                                                                                           GCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCAAAAGAGGCCAGCCGCAAAAGCAGTGCGTGCTTTAAGAGAT  <  2:167340/102‑1
                                                                                                                                                                                                 
AATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/1665074‑1665225
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttttgcggcAAAAGAGGCCAGCCGCAAAAGCAGTGCGTGCTTTAAGAGAT  <  NC_000913_3_hsa_tpiA/1665236‑1665196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.