Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913_3_hsa_tpiA	1,157,972	Δ60 bp	6.4%	coding (104‑163/1434 nt)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1157971	45 (1.010)	3 (0.070)	3/258	NT	6.4%	coding (103/1434 nt)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
	?	NC_000913_3_hsa_tpiA	1158032 =	45 (1.070)					coding (164/1434 nt)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component

TGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGT  >  NC_000913_3_hsa_tpiA/1157825‑1158119                                                                                                                                                   |                                                                                                                                                     tGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCcgcg                                                                                                                                                    >  2:80722/1‑149 (MQ=255)          aaaaaaaGCACCCATACCCAGGAGCACTTTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAACGGTCGGTAAATCGCGGAGGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAgc                                                                                                                                           <  2:510442/149‑1 (MQ=255)           aaaaaaGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGATGAGCGTCGCTTCCGCGAAGTTCCgct                                                                                                                                          >  2:485656/1‑149 (MQ=255)           aaaaaaGCACCCATACTCAGGAACACTCTCAATCATCTTCAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTCTCGCAGCTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAgct                                                                                                                                          >  2:424598/1‑149 (MQ=255)               aaGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAgctggct                                                                                                                                      >  2:792479/1‑149 (MQ=255)                     ccATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTCTGGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCccgc                                                                                                                                >  1:24257/1‑149 (MQ=255)                       aTACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTgccggccg                                                                                                                              >  1:642241/1‑149 (MQ=255)                       aTACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCccgccg                                                                                                                              >  1:247666/1‑149 (MQ=255)                           tCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGtctttgcaaacatgccactgatttttgc                                                                                                                          >  1:481544‑M1/1‑121 (MQ=255)                              ggAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATc                                                                                                                       <  1:768762/149‑1 (MQ=255)                                  cACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCAt                                                                                                                   >  2:711893/1‑149 (MQ=255)                                    ctttcaatTATGTTTAAGAATGCATTGGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCGGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGtctttgcaaacatgccactgatttttgcgatcggtgt                                                                                                                 <  2:672891‑M1/146‑38 (MQ=255)                                     tctcAATTATGTTTAAGAATGCATTTGCTAACCTGCAAACGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTGTTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATGGCATAtt                                                                                                                >  2:239419/1‑149 (MQ=255)                                     tctcAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTCCCGGTATCCGTACTGCCTATCGCAGGTATTCTGATGGGCGTCGGTGCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTCTCGCATGtt                                                                                                                >  2:836709/1‑149 (MQ=255)                                     actcAATTATGTTTCAGAATGCATTTGCTAAACTGCAAAAGGTCGGTAAATCGCTGATGGTGCCGGTATCCGTACTTCCTATCGCAAGTATTCTGCTTGGAGACGGTTACTAGAATTTCAGCTGACTGCCCGCAATTCAATCGCATGtc                                                                                                                >  2:594866/2‑148 (MQ=255)                                          aTTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGc                                                                                                           >  1:800747/1‑149 (MQ=255)                                               gTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGACTTTCAGCTGGCTGCCCGCCGTTGTATCCCCTGTTATGGCCGagg                                                                                                      >  2:470377/1‑147 (MQ=255)                                                 ggaaGAACGCGTTCGCTAACCTGTAAAAGCTCAGTAAATCGCTGATGCTGCCGGTATCCTTACTGCCTATCTCATGGATTCTGCTGTGCGTCGGTTCCGCTAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTCACGGCAGAAGCa                                                                                                    <  2:472535/147‑1 (MQ=255)                                                    aGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGc                                                                                                 <  1:242507/149‑1 (MQ=255)                                                     gAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGCGCGTCGGTTCCGCCAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGAAGACGCAGGCg                                                                                                >  2:323289/1‑149 (MQ=255)                                                          cATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGGGTCGGTCCCTGGAATTTTAGCTGGCTTCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTcc                                                                                           <  1:799172/149‑1 (MQ=255)                                                                  aaCCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGc                                                                                   >  2:835196/1‑149 (MQ=255)                                                                     cTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCaaa                                                                                >  1:36125/1‑149 (MQ=255)                                                                      tGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAAc                                                                               >  1:375244/1‑149 (MQ=255)                                                                      tGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCAGGCCGCCGTTGTATCGCATGTTATGGCCGAAGCAGGCGGTTCCGTCTTTGCAAAc                                                                               >  1:191029/1‑149 (MQ=255)                                                                       gCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTTCAAACa                                                                              >  2:754245/1‑149 (MQ=255)                                                                        cAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTGCCGTCTTTGCAAACAt                                                                             >  1:501334/1‑149 (MQ=255)                                                                           aaGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGCAGCAGGCGGTTCCGTCTTTGCAAACACGcc                                                                          >  2:522764/1‑149 (MQ=255)                                                                                     aaTCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGAttttt                                                                <  2:31315/149‑1 (MQ=255)                                                                                      aTCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTg                                                               <  1:84977/149‑1 (MQ=255)                                                                                          cTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCAATAGAGGTCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGAt                                                           >  1:400121/1‑149 (MQ=255)                                                                                            gATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGACGTTGTATCGCAGGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCg                                                         <  1:158507/149‑1 (MQ=255)                                                                                                 tgcCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGtctttgcaaacatgccactgatttttgcgatcggtgtcgccctcggctttaccaataacgatggcgtatcctcgctggccgcacttgttgcctatggc                                                    >  2:404085‑M1/1‑51 (MQ=255)                                                                                                 tgcCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTc                                                    >  1:303533/1‑149 (MQ=255)                                                                                                 tgcCGGTATCCGTACTGCCTATCGCAGGTATTCTCCTGGGCACCGGTTCCGCCAATTTCCCCTGGCTGCCCGCCGTTCTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCCCTGAATTTTGCGATCGATGTc                                                    >  2:6279/1‑149 (MQ=255)                                                                                                 tgcCGGTATCCGTACTGCCTATCGCAGGTATTCGACTGGGCGTCGGTGCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGTCAGTAGAAGGCGTGTCCGTCTTTGCAAACATGACAGTGATTTTTGCGCGCGgtgta                                                    >  2:5784/1‑148 (MQ=255)                                                                                                       tATCCGTACTGCCTATCGCAGGTATTCTGCTGAGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCGGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTc                                              >  2:196740/1‑149 (MQ=255)                                                                                                                  ccTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCaa                                   <  1:616366/149‑1 (MQ=255)                                                                                                                     aTCGCAGGTATTCTGCTGGACTTCGGGTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATaa                                >  2:707752/1‑149 (MQ=255)                                                                                                                      tCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGTTCGGTGTCGCCCTCGGCTTTACCACTagc                               >  2:730287/1‑147 (MQ=255)                                                                                                                         gaGGTATTCTGCTGGGCGGCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGAt                            <  1:185175/148‑1 (MQ=255)                                                                                                                         cAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGAt                            <  1:712259/149‑1 (MQ=255)                                                                                                                         cAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGAt                            >  1:543634/1‑149 (MQ=255)                                                                                                                          aGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCCTTACCAATAACGAtt                           >  2:606684/1‑148 (MQ=255)                                                                                                                            gTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGc                         >  1:569387/1‑149 (MQ=255)                                                                                                                            gTATTATGCTGGGCGTGGGTTCCGCTAATTTGAGCGGGCTGCCCGCCGTTGTATCGCATGGTATGGCAGAAGCAGGCGGTCCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGc                         <  2:17928/149‑1 (MQ=255)                                                                                                                                tCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTAt                     <  1:711081/149‑1 (MQ=255)                                                                                                                                       ggCGTCGGTTCCGtctttgcaaacatgccactgatttttgcgatcggtgtcgccctcggctttaccaataacgatggcgtatccgcgctggccgcagtt                                                                <  1:398356‑M1/99‑87 (MQ=255)                                                                                                                                       ggCGTCGGTTCCGtctttgcaaacatgccactgatttttgcgatcggtgtcgccctcggctttaccaataacgatggcgtatccgcgctggccgcagtt                                                                >  2:398356‑M1/1‑13 (MQ=255)                                                                                                                                       ggCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCCATAACGATGGCGTATCCGCGCt              >  1:596160/1‑149 (MQ=255)                                                                                                                                         cGTCGGTTTCGCGAATTTAAGCTGGCTGCCCGCCGTTGTATCGGATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCAACTGATGTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTgg            <  2:125178/149‑1 (MQ=255)                                                                                                                                               ttCCGtctttgcaaacatgccactgatttttgcgatcggtgtcgccctcggctttaccaataacgatggcgtatccgcgctggccgcagttgttgcctatggcatcatggttaaaaccatggccgtggttgcgccactggtactgcatt      >  1:686420‑M1/1‑5 (MQ=255)                                                                                                                                               ttCCGtctttgcaaacatgccactgatttttgcgatcggtgtcgccctcggctttaccaataacgatggcgtatccgcgctggccgcagttgttgcctatggcatcatggttaaaaccatggcagtggttgcgccactggtactgcatt      >  1:335278‑M1/1‑5 (MQ=255)                                                                                                                                                   gtctttgcaaacatgccactgatttttgcgatcggtgtcgccctcggctttaccaataacgatggcgtatccgcgctggccgcagttgttgttgcctatggcatcatggttaaaaccatggccgtggttgcgccactggtactgcattt  >  1:110436‑M1/1‑1 (MQ=255)                                                                                                                                                   gcgAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCGTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTGTACCAATAACGATGGCGTATCCGCGCTGGCCGAAgttgt  >  2:598426/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGT  >  NC_000913_3_hsa_tpiA/1157825‑1158119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.