New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	34 (0.970)	31/268	NT	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299351‑1299646                                                                                                                                                    |                                                                                                                                                     ttctCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                                     >  1:104135‑M1/1‑148 (MQ=255)    tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAatac                                                                                                                                                  >  1:452790‑M1/1‑145 (MQ=255)                          gATTACCTTAACTGCGTATACTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                                                                            >  2:667849‑M1/1‑123 (MQ=255)                              tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                                                                                                                        <  1:275268‑M1/149‑31 (MQ=255)                                cTTAACTGCTTCTCCTCACCATCGTGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                      <  1:579151‑M1/149‑33 (MQ=255)                                 ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                                                                                                                     >  1:44709‑M1/1‑116 (MQ=255)                                 ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTCAAGAATAATTGAAATAATATTATTAATTCCACTGACTTTGGTATAGGAAAGAGCAAActaataatcaattcctaaataattgttaattct                                                                                                                     >  2:105586‑M1/1‑116 (MQ=255)                                 ttAACTGCTTCTCCTCACCATCATGTTATTCTACTCTTTTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTATGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcctttc                                                                                                                     >  1:334411‑M1/1‑116 (MQ=255)                                     cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                                 >  1:625040‑M1/1‑112 (MQ=255)                                       gCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                               <  1:538943‑M1/149‑40 (MQ=255)                                             ccacaccCTCATGTTATTTTCTCCACATCATAATCCTGTGCTTGCTGAAAAATAATTGAAATGATATTATTAATTCCACTGTCTTTTGTAGAGGAAAGTGCTAAataataatcacttgttaaattattgtgcatttcactactggaact                                                                                                         <  2:282208‑M1/146‑46 (MQ=255)                                              ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGGAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaccactggaactg                                                                                                        >  1:398326‑M1/1‑103 (MQ=255)                                                      catGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAATTGCTAAataataatcaattgttaaattatagtgcatttcacaactggaactgtaatcaaa                                                                                                >  2:612309‑M1/1‑95 (MQ=255)                                                             ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                         <  1:512610‑M1/149‑62 (MQ=255)                                                               ttCGCCACATCATAACCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                       <  1:14945‑M1/149‑64 (MQ=255)                                                                    cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                                  >  1:137154‑M1/1‑81 (MQ=255)                                                                       atcatAATCCTGGGCTTGCTGAGGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactacttgaactgtaatcagaaaagatagccatgctta                                                                               >  2:394276‑M1/1‑78 (MQ=255)                                                                             agcctgGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaat                                                                         <  1:487446‑M1/147‑78 (MQ=255)                                                                                      ttGCTGAAGAATAATTGAAATGATATTATTACTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgagactgtcatccgcaaacctagacatgtgtagccaatctccggttt                                                                >  2:132101‑M1/1‑63 (MQ=255)                                                                                       gtctgAAGAAGTATTGAAATGATATTAGTAATTCCACTGCCTTTGGTAGAGGAAAGTGTTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgtttagccaatctctatttga                                                               <  2:80681‑M1/147‑88 (MQ=255)                                                                                                  aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                    <  2:272354‑M1/149‑99 (MQ=255)                                                                                                   atTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatatctatttgattgaattgaaag                                                   >  2:211216‑M1/1‑50 (MQ=255)                                                                                                       aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactagaactgtaatcagataagatagacatgcttagccaatctctatttgattgaattgaaagatgt                                               <  1:605248‑M1/149‑104 (MQ=255)                                                                                                              attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                        <  1:304770‑M1/149‑111 (MQ=255)                                                                                                                tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                      <  1:570006‑M1/149‑113 (MQ=255)                                                                                                                tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                                  <  1:571888‑M1/89‑53 (MQ=255)                                                                                                                tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                                  >  2:571888‑M1/1‑37 (MQ=255)                                                                                                                  ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                                    >  1:464035‑M1/1‑35 (MQ=255)                                                                                                                     aTTCCTCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                                 <  1:187791‑M1/149‑118 (MQ=255)                                                                                                                       tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                               <  1:461513‑M1/149‑120 (MQ=255)                                                                                                                            tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                          <  1:635879‑M1/149‑125 (MQ=255)                                                                                                                              ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                        >  1:245940‑M1/1‑23 (MQ=255)                                                                                                                                tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                      >  1:404859‑M1/1‑21 (MQ=255)                                                                                                                                tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattagattgaattaaaagatgtttgttaaggcatggatgcaagctat                      >  1:17504‑M1/1‑21 (MQ=255)                                                                                                                                      agaagaAAGTGCAAAataaaaattaattgttaaattattttgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgacttgaaagatgtttgttaaggtatggatgcaagctatagattc                <  2:6212‑M1/149‑135 (MQ=255)                                                                                                                                       gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattct               >  1:556490‑M1/1‑14 (MQ=255)                                                                                                                                               tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt       <  1:63443‑M1/149‑144 (MQ=255)                                                                                                                                                    aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaata  >  1:199887‑M1/1‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_hsa_tpiA/1299351‑1299646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.