New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1299498	0 (0.000)	34 (0.970)	31/268	NT	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_hsa_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_hsa_tpiA/1300550‑1300845                                                                                                                                                     |                                                                                                                                                    ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                     >  1:104135‑M2/149‑149 (MQ=255)    tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaatac                                                                                                                                                  >  1:452790‑M2/146‑148 (MQ=255)                          gattaccttaactgcgtatactcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                            >  2:667849‑M2/124‑149 (MQ=255)                              tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAt                                                                                                                        <  1:275268‑M2/30‑1 (MQ=255)                                cttaactgcttctcctcaccatcgtgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                      <  1:579151‑M2/32‑1 (MQ=255)                                 ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTc                                                                                                                     >  1:44709‑M2/117‑149 (MQ=255)                                 ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctcaagaataattgaaataatattattaattccactgactttggtataggaaagagcaaaCTAATAATCAATTCCTaaataattgttaattct                                                                                                                     >  2:105586‑M2/117‑142 (MQ=255)                                 ttaactgcttctcctcaccatcatgttattctactcttttcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctatggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCCTTTc                                                                                                                     >  1:334411‑M2/117‑149 (MQ=255)                                     ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                                 >  1:625040‑M2/113‑149 (MQ=255)                                       gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                               <  1:538943‑M2/39‑1 (MQ=255)                                             ccacaccctcatgttattttctccacatcataatcctgtgcttgctgaaaaataattgaaatgatattattaattccactgtcttttgtagaggaaagtgctaaATAATAATCACTTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                         <  2:282208‑M2/45‑1 (MQ=255)                                              ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgggagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACCACTGGAACTg                                                                                                        >  1:398326‑M2/104‑149 (MQ=255)                                                      catgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaattgctaaATAATAATCAATTGTTAAATTATAGTGCATTTCACAACTGGAACTGTAAtcaaa                                                                                                >  2:612309‑M2/96‑147 (MQ=255)                                                             ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                         <  1:512610‑M2/61‑1 (MQ=255)                                                               ttcgccacatcataaccctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                       <  1:14945‑M2/63‑1 (MQ=255)                                                                    cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                                  >  1:137154‑M2/82‑149 (MQ=255)                                                                       atcataatcctgggcttgctgaggaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTTGAACTGTAATCAGAAAAGATAGCCATGCTTa                                                                               >  2:394276‑M2/79‑149 (MQ=255)                                                                             agcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAt                                                                         <  1:487446‑M2/77‑1 (MQ=255)                                                                                      ttgctgaagaataattgaaatgatattattacttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGAGACTGTCATCCGCAAACCTAGACATGTGTagccaatctccggttt                                                                >  2:132101‑M2/64‑143 (MQ=255)                                                                                       gtctgaagaagtattgaaatgatattagtaattccactgcctttggtagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGTTTAGCCAATCTCTATttga                                                               <  2:80681‑M2/87‑1 (MQ=255)                                                                                                  aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                                    <  2:272354‑M2/98‑1 (MQ=255)                                                                                                   attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATATCTATTTGATTGAATTGAAAg                                                   >  2:211216‑M2/51‑149 (MQ=255)                                                                                                       aaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTAGAACTGTAATCAGATAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgt                                               <  1:605248‑M2/103‑1 (MQ=255)                                                                                                              attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                        <  1:304770‑M2/110‑1 (MQ=255)                                                                                                                tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                                  <  1:571888‑M2/52‑1 (MQ=255)                                                                                                                tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                                  >  2:571888‑M2/38‑89 (MQ=255)                                                                                                                tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAgg                                      <  1:570006‑M2/112‑1 (MQ=255)                                                                                                                  ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCa                                    >  1:464035‑M2/36‑149 (MQ=255)                                                                                                                     attcctctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                                 <  1:187791‑M2/117‑1 (MQ=255)                                                                                                                       tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                               <  1:461513‑M2/119‑1 (MQ=255)                                                                                                                            tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                          <  1:635879‑M2/124‑1 (MQ=255)                                                                                                                              cctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCt                        >  1:245940‑M2/24‑149 (MQ=255)                                                                                                                                tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                      >  1:404859‑M2/22‑149 (MQ=255)                                                                                                                                tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTAGATTGAATTAAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                      >  1:17504‑M2/22‑149 (MQ=255)                                                                                                                                      agaagaaagtgcaaaATAAAAATTAATTGTTAAATTATTTTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGACTTGAAAGATGTTTGTTAAGGTATGGATGCAAGCTATAGATTc                <  2:6212‑M2/134‑1 (MQ=255)                                                                                                                                       gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt               >  1:556490‑M2/15‑149 (MQ=255)                                                                                                                                               tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt       <  1:63443‑M2/143‑1 (MQ=255)                                                                                                                                                    aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa  >  1:199887‑M2/2‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_hsa_tpiA/1300550‑1300845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.