New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 3181870	36 (1.080)	5 (0.160)	5/266	3.7	12.8%	coding (538/789 nt)	ygiD	4,5‑ DOPA‑extradiol‑dioxygenase
	?	NC_000913_3_hsa_tpiA	= 3181874	34 (1.070)					coding (534/789 nt)	ygiD	4,5‑ DOPA‑extradiol‑dioxygenase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACGGCAAATAATGCTCTGGCGTTGGGTTCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGAT  >  NC_000913_3_hsa_tpiA/3181723‑3182008                                                                                                                                                    |                                                                                                                                           acGGCAAATAATGCTCTGGCGTTGGGTTCGATAACGTGCCACCCTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGaa                                                                                                                                           <  1:288728/149‑1 (MQ=255)               tctGGCGTTGGGTTCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCCTTAAACGACGTCGCGAACGGGTACGGTGAACTATCACCGTGCCa                                                                                                                             <  2:215563/149‑1 (MQ=255)                           ttCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTgcg                                                                                                                 >  1:273074/1‑149 (MQ=255)                                tAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACCTCACTGTGCGCAGGt                                                                                                            <  2:491442/149‑1 (MQ=255)                                    gTGCCACTTTCATGGTCGAGGTAATTCACCAGAGGCTGTTGTTCCACCGCCGCTGGCCACGTCAGATTCGCTTTCACATACTCATTACACTACGTCGCCCACGGATACGGTGAACTTTCACCGTGCCACTTCACTGTGCGCAGGTTATg                                                                                                        <  1:164750/149‑1 (MQ=255)                                                                ccAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCAc                                                                                                                                    >  1:196600/1‑93 (MQ=255)                                                                ccAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCAc                                                                                                                                    <  2:196600/93‑1 (MQ=255)                                                                ccAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATACACCACGTTACCGGTGGCGACAAACaat                                                                            >  2:417927/1‑147 (MQ=255)                                                                   gagGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACcttatt                                                                         >  2:11886/1‑149 (MQ=255)                                                                   gagGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACattatt                                                                         <  1:239404/149‑1 (MQ=255)                                                                   gagGATGCTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACattatt                                                                         <  1:239863/149‑1 (MQ=255)                                                                      gATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCt                                                                      <  1:38642/149‑1 (MQ=255)                                                                        tgttgtTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCGCTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTc                                                                    <  1:249901/149‑1 (MQ=255)                                                                             ttCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGGGCCACGTTAACGCTGGCGACCAACATTATTCCTTCAtctc                                                               >  1:78821/1‑149 (MQ=255)                                                                             ttCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGAc                                                                                   >  2:4802/1‑129 (MQ=255)                                                                             ttCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGAc                                                                                   <  1:4802/129‑1 (MQ=255)                                                                                       ccTTGCCAGGTCAGATTCGCTTTCACATACTCATTAAACGACGGCGCCCACGGATACGGTGGACTATCACCGTGCCACTTCAATGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGc                                                     <  2:181331/149‑1 (MQ=255)                                                                                             cACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCCCTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGttt                                               <  2:347976/149‑1 (MQ=255)                                                                                             cACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTCAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACATTACCGCTGGCGACCAACATTATTCCTTCATCACGCAGCGCTGCCAGttt                                               >  2:94107/1‑149 (MQ=255)                                                                                              aCGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTg                                              >  1:483120/1‑149 (MQ=255)                                                                                                     aTTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCGGGTTATGCACCACGTTACCGCTGGCGACTACCATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCa                                       >  2:517642/1‑149 (MQ=255)                                                                                                     aTTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCCCGCAGCGCTCCCAGTTTGCGCCCCa                                       >  2:543135/1‑149 (MQ=255)                                                                                                                   aCTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAg                         >  2:182564/1‑149 (MQ=255)                                                                                                                   aCTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGAGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAGTGCCAg                         >  1:190170/1‑149 (MQ=255)                                                                                                                       aTTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAggcgg                     >  1:424459/1‑149 (MQ=255)                                                                                                                           aaCGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACtgt                                                                                                                    <  1:584360/50‑1 (MQ=255)                                                                                                                           aaCGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACtgt                                                                                                                    >  2:584360/1‑50 (MQ=255)                                                                                                                                  tCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCGGTTTTGCTa          >  2:3307/1‑149 (MQ=255)                                                                                                                                   cGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCACGTTTCCTAc         >  2:236964/1‑149 (MQ=255)                                                                                                                                    gCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTg                                                                                        >  1:22767/1‑69 (MQ=255)                                                                                                                                    gCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTg                                                                                        <  2:22767/69‑1 (MQ=255)                                                                                                                                    gCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCGGCGCTGCCAGTTTGGGCCCCATTTCGAAATGCCCGGCGGCGGGTTTGCTACt        >  1:140904/1‑149 (MQ=255)                                                                                                                                      ccACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGt                <  2:438991/139‑1 (MQ=255)                                                                                                                                      ccACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGt                >  1:438991/1‑139 (MQ=255)                                                                                                                                         cGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGa   >  1:71062/1‑149 (MQ=255)                                                                                                                                          ggATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAGTAGTCAAGCGGCAGGTTTGCTACTGTCGAt  >  1:159964/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                           ACGGCAAATAATGCTCTGGCGTTGGGTTCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGAT  >  NC_000913_3_hsa_tpiA/3181723‑3182008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.