New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 3181870	36 (1.080)	5 (0.160)	5/266	3.7	12.8%	coding (538/789 nt)	ygiD	4,5‑ DOPA‑extradiol‑dioxygenase
	?	NC_000913_3_hsa_tpiA	= 3181874	34 (1.070)					coding (534/789 nt)	ygiD	4,5‑ DOPA‑extradiol‑dioxygenase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCTGGCGTTGGGTTCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCA  >  NC_000913_3_hsa_tpiA/3181737‑3182022                                                                                                                                          |                                                                                                                                                     tctGGCGTTGGGTTCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCCTTAAACGACGTCGCGAACGGGTACGGTGAACTATCACCGTGCCa                                                                                                                                           <  2:215563/149‑1 (MQ=255)             ttCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTgcg                                                                                                                               >  1:273074/1‑149 (MQ=255)                  tAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACCTCACTGTGCGCAGGt                                                                                                                          <  2:491442/149‑1 (MQ=255)                      gTGCCACTTTCATGGTCGAGGTAATTCACCAGAGGCTGTTGTTCCACCGCCGCTGGCCACGTCAGATTCGCTTTCACATACTCATTACACTACGTCGCCCACGGATACGGTGAACTTTCACCGTGCCACTTCACTGTGCGCAGGTTATg                                                                                                                      <  1:164750/149‑1 (MQ=255)                                                  ccAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCAc                                                                                                                                                  >  1:196600/1‑93 (MQ=255)                                                  ccAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCAc                                                                                                                                                  <  2:196600/93‑1 (MQ=255)                                                  ccAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATACACCACGTTACCGGTGGCGACAAACaat                                                                                          >  2:417927/1‑147 (MQ=255)                                                     gagGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACcttatt                                                                                       >  2:11886/1‑149 (MQ=255)                                                     gagGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACattatt                                                                                       <  1:239404/149‑1 (MQ=255)                                                     gagGATGCTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACattatt                                                                                       <  1:239863/149‑1 (MQ=255)                                                        gATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCt                                                                                    <  1:38642/149‑1 (MQ=255)                                                          tgttgtTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCGCTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTc                                                                                  <  1:249901/149‑1 (MQ=255)                                                               ttCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGGGCCACGTTAACGCTGGCGACCAACATTATTCCTTCAtctc                                                                             >  1:78821/1‑149 (MQ=255)                                                               ttCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGAc                                                                                                 >  2:4802/1‑129 (MQ=255)                                                               ttCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGAc                                                                                                 <  1:4802/129‑1 (MQ=255)                                                                         ccTTGCCAGGTCAGATTCGCTTTCACATACTCATTAAACGACGGCGCCCACGGATACGGTGGACTATCACCGTGCCACTTCAATGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGc                                                                   <  2:181331/149‑1 (MQ=255)                                                                               cACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCCCTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGttt                                                             <  2:347976/149‑1 (MQ=255)                                                                               cACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTCAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACATTACCGCTGGCGACCAACATTATTCCTTCATCACGCAGCGCTGCCAGttt                                                             >  2:94107/1‑149 (MQ=255)                                                                                aCGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTg                                                            >  1:483120/1‑149 (MQ=255)                                                                                       aTTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCGGGTTATGCACCACGTTACCGCTGGCGACTACCATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCa                                                     >  2:517642/1‑149 (MQ=255)                                                                                       aTTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCCCGCAGCGCTCCCAGTTTGCGCCCCa                                                     >  2:543135/1‑149 (MQ=255)                                                                                                     aCTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAg                                       >  2:182564/1‑149 (MQ=255)                                                                                                     aCTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGAGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAGTGCCAg                                       >  1:190170/1‑149 (MQ=255)                                                                                                         aTTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAggcgg                                   >  1:424459/1‑149 (MQ=255)                                                                                                             aaCGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACtgt                                                                                                                                  <  1:584360/50‑1 (MQ=255)                                                                                                             aaCGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACtgt                                                                                                                                  >  2:584360/1‑50 (MQ=255)                                                                                                                    tCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCGGTTTTGCTa                        >  2:3307/1‑149 (MQ=255)                                                                                                                     cGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCACGTTTCCTAc                       >  2:236964/1‑149 (MQ=255)                                                                                                                      gCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTg                                                                                                      <  2:22767/69‑1 (MQ=255)                                                                                                                      gCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTg                                                                                                      >  1:22767/1‑69 (MQ=255)                                                                                                                      gCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCGGCGCTGCCAGTTTGGGCCCCATTTCGAAATGCCCGGCGGCGGGTTTGCTACt                      >  1:140904/1‑149 (MQ=255)                                                                                                                        ccACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGt                              <  2:438991/139‑1 (MQ=255)                                                                                                                        ccACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGt                              >  1:438991/1‑139 (MQ=255)                                                                                                                           cGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGa                 >  1:71062/1‑149 (MQ=255)                                                                                                                            ggATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAGTAGTCAAGCGGCAGGTTTGCTACTGTCGAt                >  1:159964/1‑149 (MQ=255)                                                                                                                                          aTCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATCCTCAACTGCACCa  >  2:384245/1‑149 (MQ=255)                                                                                                                                          |                                                                                                                                                     TCTGGCGTTGGGTTCGATAACGTGCCACCTTCATGGTCGAGGTAATTCACCAGAGGATGTTGTTCCACTGGCCCTTGCCACGTCAGATTCGCTTTCACATACTCATTAAACGACGTCGCCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCA  >  NC_000913_3_hsa_tpiA/3181737‑3182022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.