New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 3248531 =43 (0.920)6 (0.140) 6/264 5.1 12.8% coding (93/384 nt) mzrA modulator of EnvZ/OmpR regulon
?NC_000913_3_hsa_tpiA 3248557 = 41 (0.930)coding (119/384 nt) mzrA modulator of EnvZ/OmpR regulon
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCACCCCCAAAAACGACACTCTGTTAATTACATTT  >  NC_000913_3_hsa_tpiA/3248388‑3248678
                                                                                                                                               |                                                                                                                                                   
ggTTCGCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCa                                                                                                                                                <  1:706783/149‑1 (MQ=255)
 gTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAg                                                                                                                                               <  1:749545/149‑1 (MQ=255)
    ctctGGTCTTGTGACGGAAAGAGAAGCGGGGAAACCGGGGGGAAGGGATGCAAATACCTCGCATGTCGCTTCGCCATCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCaa                                                                                                                                            <  1:150584/149‑1 (MQ=255)
      ctGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAaga                                                                                                                                          <  2:318828/149‑1 (MQ=255)
        ggTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGt                                                                                                                                        <  1:477892/149‑1 (MQ=255)
                 aTGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAATAGTCTACGCTgg                                                                                                                               >  2:410947/1‑149 (MQ=255)
                   ggAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCg                                                                                                                             >  1:341607/1‑149 (MQ=255)
                     aaaaaGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGAt                                                                                                                           <  1:416654/149‑1 (MQ=255)
                      aaaaGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGAtt                                                                                                                          >  1:554727/1‑149 (MQ=255)
                       aaaGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTc                                                                                                                         >  2:341419/1‑149 (MQ=255)
                         aGAAATATGGAAATCGGGGGTAAGGGATGCAAATCCCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTGCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGt                                                                                                                       <  1:323764/149‑1 (MQ=255)
                           aaaTATGGAAATCGGGGGTAAGGCATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGc                                                                                                                     <  1:672534/149‑1 (MQ=255)
                             atatGGATATCGGGGGTAAGGGATGAAAATACCTCCCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCg                                                                                                                   <  2:341607/149‑1 (MQ=255)
                                 ggAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTtca                                                                                                               >  1:784418/1‑149 (MQ=255)
                                      tCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAg                                                                                                          <  1:213980/149‑1 (MQ=255)
                                       tggaggTAAGGTATGCAAGTACCTCGCATTTCGTTTCGCTAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAgg                                                                                                         <  2:165212/145‑1 (MQ=255)
                                       cGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAgg                                                                                                         <  2:814378/149‑1 (MQ=255)
                                           ggTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGTTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGcacc                                                                                                     >  1:197329/1‑148 (MQ=255)
                                                gggATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTAGGCACGCGGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGAt                                                                                                <  2:711118/149‑1 (MQ=255)
                                                 ggATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAACGCACCACGATg                                                                                               >  1:430930/1‑149 (MQ=255)
                                                    tGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCa                                                                                            <  1:485232/149‑1 (MQ=255)
                                                      cAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGa                                                                                          <  1:254987/149‑1 (MQ=255)
                                                        aaTTCCTCGCATGTCGCTTGGCCAGCTAGCCTGGTCCGGGGCTGTTTTACTTCTGGTCGGTATGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACg                                                                                        <  2:569594/149‑1 (MQ=255)
                                                        aaTACCTCGCATGTCGCTTCGCCCGATAGCATAATCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACg                                                                                        >  2:594540/1‑149 (MQ=255)
                                                              tCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCCCAAAGATGCCAGACGGttttt                                                                                  >  1:125784/1‑149 (MQ=255)
                                                                   tGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTTGTCGGCAGGGTGTTCCGGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATc                                                                             <  2:149810/149‑1 (MQ=255)
                                                                      cGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTgg                                                                          <  1:512999/149‑1 (MQ=255)
                                                                               aGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCCCCtt                                                                 >  2:187232/1‑149 (MQ=255)
                                                                                  tAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGAc                                                              >  1:5332/1‑149 (MQ=255)
                                                                                  tAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGAc                                                              <  1:341419/149‑1 (MQ=255)
                                                                                  tAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATAAAAGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTgcc                                                              >  1:21274/1‑147 (MQ=255)
                                                                                      cTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTGGACAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTc                                                          >  2:302350/1‑149 (MQ=255)
                                                                                                   gTTTTACTTCTGGTCGGCACCCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCttt                                             <  2:548813/149‑1 (MQ=255)
                                                                                                    ttttACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTc                                            >  2:774200/1‑149 (MQ=255)
                                                                                                      ttACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCaa                                          >  1:592633/1‑149 (MQ=255)
                                                                                                                tCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCAcc                                <  2:5332/149‑1 (MQ=255)
                                                                                                                   gCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCAccccc                             >  2:467247/1‑149 (MQ=255)
                                                                                                                   gCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCAccccc                             >  2:594733/1‑149 (MQ=255)
                                                                                                                     aCGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCACCCCCaa                           >  1:311703/1‑149 (MQ=255)
                                                                                                                          gTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCACCCCCAAAAACg                      >  1:324690/1‑149 (MQ=255)
                                                                                                                                  ccTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCACCCCCAAAAACGACACTCTg              <  1:594733/149‑1 (MQ=255)
                                                                                                                                      gTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCACCCCCAAAAACGACACTCTGTTaa          <  2:507110/149‑1 (MQ=255)
                                                                                                                                              ttCCCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCACCCCCAAAAACGACACTCTGTTAATTACAttt  <  1:252661/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGGTCCGCGGTTCGCCAGCAAGAGTCTACGCTGGCGATTCGTGCCGTTCATCAAGGCACAACGATGCCAGACGGTTTTTCAATCTGGCATCACCTTGACGCTCATGGCATTCCTTTCAAAAGTATCACCCCCAAAAACGACACTCTGTTAATTACATTT  >  NC_000913_3_hsa_tpiA/3248388‑3248678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.