New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 377527NA (NA)4 (0.140) 4/274 3.5 10.8% intergenic (+216/+8) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
?NC_000913_3_pae_tpiA = 377605 35 (1.190)coding (764/834 nt) frmB S‑formylglutathione hydrolase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTG  >  NC_000913_3_pae_tpiA/377380‑377657
                                                                                                                                                   |                                                                                                                                  
ggCGAAGTGTAGGTCAGATAAGGCGTTCACGGCCCATGCGGCAGTCGTGGGACCTGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTg                                                                                                                                   <  2:89870/149‑1 (MQ=18)
ggCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTGGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGta                                                                                                                                   >  2:313875/1‑148 (MQ=255)
ggCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTg                                                                                                                                   <  1:320002/149‑1 (MQ=255)
 gCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCGGTCGTGc                                                                                                                                  >  1:370981/1‑149 (MQ=255)
     aGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCtatt                                                                                                                              <  1:237759/149‑1 (MQ=255)
         tAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCtattattt                                                                                                                          >  2:205974/1‑147 (MQ=255)
                  tAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATAtt                                                                                                                 >  2:493205/1‑149 (MQ=255)
                  tAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGATAACGCCGCATCCGTCTGTCGTGCTATTATCAACGCATAtt                                                                                                                 >  1:143287/1‑149 (MQ=255)
                      gCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGt                                                                                                             <  1:473324/149‑1 (MQ=255)
                        gTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGttt                                                                                                           >  2:421195/1‑149 (MQ=255)
                                         cAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCaa                                                                                          <  1:294299/149‑1 (MQ=255)
                                         cAGTCGGGCGCGAGGCCGGATGGGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCaa                                                                                          <  2:468428/149‑1 (MQ=21)
                                          aGTCGTGCGTCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAat                                                                                         <  2:322962/149‑1 (MQ=255)
                                              gTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGACCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCAGCCGGCAGTCGTGCTATTATCAACGCATATTCATTTTATTGACGGGGGAGTCAatatac                                                                                     >  1:242196/1‑147 (MQ=21)
                                               tGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCt                                                                                    >  2:15749/1‑149 (MQ=255)
                                                             tGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTg                                                                      <  1:290032/149‑1 (MQ=255)
                                                                 aCGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAGTCGTGCTATTATCAACGAATATTCAGTTTATTGTCGTGGTAGCCAAAAAGCTAGCCAATAAAACTGgaga                                                                  >  1:295736/1‑149 (MQ=25)
                                                                 aCGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTAGCGAATGAAACTGgaga                                                                  >  1:295675/1‑149 (MQ=255)
                                                                      ttGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACaaaa                                                             <  2:315994/149‑1 (MQ=255)
                                                                      ttGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACaaaa                                                             <  1:57180/149‑1 (MQ=255)
                                                                          gcgTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATAtt                                                                                                                 >  1:353372/1‑93 (MQ=255)
                                                                          gcgTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATAtt                                                                                                                 <  2:353372/93‑1 (MQ=255)
                                                                          gcgTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAaataat                                                         >  1:25111/1‑149 (MQ=255)
                                                                              tttATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCt                                                     <  2:139794/148‑1 (MQ=255)
                                                                                  tCAAGCCTATGGATTACGGGTGAAGGGTAGGCCGAATAAGGCGTTCACGACGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGCCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGa                                                 <  1:342930/149‑1 (MQ=38)
                                                                                         tACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAAc                                          <  1:178147/149‑1 (MQ=255)
                                                                                                    ggCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATaa                               <  1:235818/149‑1 (MQ=255)
                                                                                                     gCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAAc                              >  1:325598/1‑149 (MQ=255)
                                                                                                          gtgtAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGAAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGAtt                         <  1:15749/149‑1 (MQ=255)
                                                                                                                gCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTc                   >  1:197231/1‑149 (MQ=255)
                                                                                                                      tAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGata             <  1:346467/149‑1 (MQ=255)
                                                                                                                       aaGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGatat            <  1:493205/149‑1 (MQ=255)
                                                                                                                       aaGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGCGGTCGGGATTAAGCTCGCCAATAAAGCTGGAGACAAAATCATAGGTGTCATCCTCACGCGCGTGCTAACGGAGTAACGTCtttgtct            >  1:15552/1‑144 (MQ=255)
                                                                                                                                aCGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACACTCGTGATAACGGATTAACGTCTTGATACTAATATCCt   >  2:323038/1‑149 (MQ=255)
                                                                                                                                 cgccgcATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTg  >  1:38952/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                  
GGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTG  >  NC_000913_3_pae_tpiA/377380‑377657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.