New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 377527	NA (NA)	4 (0.140)	4/274	3.5	10.8%	intergenic (+216/+8)	yaiL/frmB	DUF2058 family protein/S‑formylglutathione hydrolase
	?	NC_000913_3_pae_tpiA	= 377605	35 (1.190)					coding (764/834 nt)	frmB	S‑formylglutathione hydrolase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAATCATCACTCAACCCCTGATCAACCATGATTTCCGCAACGCGTTGACC  >  NC_000913_3_pae_tpiA/377458‑377750                                                                                                                                                    |                                                                                                                                                  tttATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCt                                                                                                                                                  <  2:139794/148‑1 (MQ=255)     tCAAGCCTATGGATTACGGGTGAAGGGTAGGCCGAATAAGGCGTTCACGACGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGCCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGa                                                                                                                                              <  1:342930/149‑1 (MQ=38)            tACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAAc                                                                                                                                       <  1:178147/149‑1 (MQ=255)                       ggCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATaa                                                                                                                            <  1:235818/149‑1 (MQ=255)                        gCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAAc                                                                                                                           >  1:325598/1‑149 (MQ=255)                             gtgtAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGAAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGAtt                                                                                                                      <  1:15749/149‑1 (MQ=255)                                   gCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTc                                                                                                                >  1:197231/1‑149 (MQ=255)                                         tAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGata                                                                                                          <  1:346467/149‑1 (MQ=255)                                          aaGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGatat                                                                                                         <  1:493205/149‑1 (MQ=255)                                          aaGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGCGGTCGGGATTAAGCTCGCCAATAAAGCTGGAGACAAAATCATAGGTGTCATCCTCACGCGCGTGCTAACGGAGTAACGTCtttgtct                                                                                                         >  1:15552/1‑144 (MQ=255)                                                   aCGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACACTCGTGATAACGGATTAACGTCTTGATACTAATATCCt                                                                                                >  2:323038/1‑149 (MQ=255)                                                    cgccgcATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTg                                                                                               >  1:38952/1‑149 (MQ=255)                                                                        cTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTg                                                                           <  1:313875/149‑1 (MQ=255)                                                                                     atatTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTATTAATATTCATCTCCTGGAAGATCTTTTCAAGCTTTGGAGTACGCAGCTg                                                              >  2:472102/1‑149 (MQ=255)                                                                                        ttCAGTTTTTTTGCGTGGAAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTc                                                           <  1:353059/149‑1 (MQ=255)                                                                                        ttCAGTTTATTGGCGTGGTAGGCAATAGGCTCGCCCACAAAACTGGACACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATGCACCTCTCGATATTCATCTCCTTGCAGCTCTTTTCAAACGGTGGATACCGCAGCTGtgc                                                           >  2:92826/1‑147 (MQ=255)                                                                                        ttCAGTTTATGGGCGATGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGTATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTc                                                           <  2:66421/149‑1 (MQ=255)                                                                                           aGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATGTTCATCTCCTGGCAGATCTCTTCAAGATATGGAGGCCGCTGCCGTTCtac                                                        >  2:429027/1‑147 (MQ=255)                                                                                                    gCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCCGCTGTTCTGCGTAAAAatc                                               >  1:245601/1‑149 (MQ=255)                                                                                                     cGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAatca                                              <  2:38952/149‑1 (MQ=255)                                                                                                      gTGGTCGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCTCAGCTGTTCTGCGTAAAAatcat                                             <  1:195528/149‑1 (MQ=255)                                                                                                      gTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAatcat                                             >  1:152019/1‑149 (MQ=255)                                                                                                       tGGTAGGCAATATGCTCGCCAATAAAACAGGACCCACGCGGGGAGCTGTGATCATAACCCTCGTGGTAACGGCTTAAGGTCTTGATATTCATATCCTGGCAGATCTTTTTAAGTTTTGGAGTCCGCAGCTGTTCTGCATAGAGatcatc                                            >  2:504455/1‑149 (MQ=255)                                                                                                            ggCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAGTCctcactca                                       >  1:80560/1‑149 (MQ=255)                                                                                                                   tGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGAttt                                                                            >  1:484753/1‑105 (MQ=255)                                                                                                                   tGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGAttt                                                                            <  2:484753/105‑1 (MQ=255)                                                                                                                        gCCAAAAAAACTCGAGGCAAAATAATAGCTTTCACTATACCCCGCGTGATAGAGGATTAACGGCTTGATACTCATCTCCTTGCAAAGCTTTGCAACAATTTACGTCAGACGAAGTTCTGCGTAAAAATCATCACTCATCTCCtcttcga                           <  2:387484/149‑7 (MQ=255)                                                                                                                         agaaTAAAACAGAAGAAAAAAAAGTGGTTGGCAGCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCCGCTGTTCTGCGTAAAAATCATCACTCAACCCCTGATCAAc                          <  2:108269/147‑1 (MQ=255)                                                                                                                           aaTAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATCCATCTCCTCGCAGATCTTTTCAAGATTTGAAGTCCCCAGCTGTTCTGCGTAAAAATCACACCCCAACCCCTGAACAACCa                        >  2:127674/1‑149 (MQ=255)                                                                                                                             tAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAATCATCACTCAACCCCTGATCAACCATg                      >  1:160867/1‑149 (MQ=255)                                                                                                                                     gagaCAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAATCATCACTCAACCCCTGATCAACCATGATTTCCGc              >  2:210008/1‑149 (MQ=255)                                                                                                                                       gaCAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCAGTTCTGCGTAAAAATCATCACTCAAACCCTGATCAACCATGATTTCCGCaa            >  1:60336/1‑149 (MQ=255)                                                                                                                                        aCAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGAAGTCCGCAGCTGTTCTGCGTAAAAATAATCAATCAACACCTGATCAACCATGAATTCAGCCAc           >  2:24072/1‑149 (MQ=255)                                                                                                                                             ataataGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAATCATCACTCAACCCCTGATCAACCATGATTTCCGCAACGCGtt      <  1:324699/149‑1 (MQ=255)                                                                                                                                                 taGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAATCATCACTCAACCCCTGATCAACCATGATTTCCGCAACGCGTTGAcc  >  1:381921/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGTCCGCAGCTGTTCTGCGTAAAAATCATCACTCAACCCCTGATCAACCATGATTTCCGCAACGCGTTGACC  >  NC_000913_3_pae_tpiA/377458‑377750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.