Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	27 [0]	[0] 27	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	26 (0.930)	24/274	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

TACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_pae_tpiA/1978368‑1978648                                                                                                                                       |                                                                                                                                                   tACACTTATCTAAGGTTTTTCCTAAATCGACGGAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACAGAAAACCCTCAAGTACTTAACATAAATGtataagtcatactt                                                                                                                                      <  2:47789‑M1/149‑15 (MQ=255)  acacTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttt                                                                                                                                     >  1:265343‑M1/1‑134 (MQ=255)         tCTAAGATTTTGCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACAAAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttg                                                                                                                              <  2:218203‑M1/149‑23 (MQ=255)                      cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                 >  2:486094‑M1/1‑114 (MQ=255)                        aaaCCGACGCAACTGTACCCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatct                                                                                                               <  1:456651‑M1/149‑38 (MQ=255)                                  aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagt                                                                                                     >  1:419055‑M1/1‑102 (MQ=255)                                         cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCAAACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgg                                                                                              >  1:310735‑M1/1‑95 (MQ=255)                                           cGTCACTACACCCACATACAACCGAGGGTGGCTCCCACTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACCTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcta                                                                                            <  2:186785‑M1/149‑57 (MQ=255)                                            gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcattctgttaaaaagttttttgcttc                                                                                           >  1:271972‑M1/1‑92 (MQ=255)                                            gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaggtttttcgctac                                                                                           >  1:271980‑M1/1‑92 (MQ=255)                                             tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATAAAACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttaaatctgttaaaaaggttttcgttacg                                                                                          >  1:68181‑M1/1‑91 (MQ=255)                                              cACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgttacgc                                                                                         <  2:453586‑M1/149‑60 (MQ=255)                                                    acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACGCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttgtcgctacgctagcaa                                                                                   >  2:359465‑M1/1‑84 (MQ=255)                                                        acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGCTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatgcttttgttttgggtgtatttcaatgtgttagaaagtttttcgctaggcgcgcaagcaa                                                                               >  1:257518‑M1/1‑80 (MQ=255)                                                           tACAACGGATGGTGGCTGCGAATTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataaggcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                            <  2:257518‑M1/149‑73 (MQ=255)                                                                          cTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                             >  1:32043‑M1/1‑62 (MQ=255)                                                                              gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaa                                                         >  1:306120‑M1/1‑58 (MQ=255)                                                                               aTTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaa                                                        >  1:114286‑M1/1‑57 (MQ=255)                                                                                   tCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaattaaacaggaataatcgaaattgg                                                    >  2:426390‑M1/1‑53 (MQ=255)                                                                                                           acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaataatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaatcact                            >  2:326738‑M1/1‑29 (MQ=255)                                                                                                           acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaaagacaaaataact                            >  2:361604‑M1/1‑29 (MQ=255)                                                                                                                    tccattaCTTAACATAAATTtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgtgcacagtcaaaataactcaccgtaaa                   <  2:512579‑M1/146‑130 (MQ=255)                                                                                                                    tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtaaaaataactcacactatg                   >  2:259974‑M1/1‑20 (MQ=255)                                                                                                                       aTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataa                >  1:516813‑M1/1‑17 (MQ=255)                                                                                                                           cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgctcagtcaaaataactcaccgtaaataatcat            >  2:173182‑M1/1‑13 (MQ=255)                                                                                                                              aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctg         <  2:22825‑M1/149‑140 (MQ=255)                                                                                                                                     aTGcataagtcatacttttgttttgtgtgtacttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaaagaaccaggaataaccgaaatgggatgttgcgcccagtcaaaataactcaccgtaaataatcatctgctataaa  <  2:51743‑M1/149‑147 (MQ=255)                                                                                                                                       |                                                                                                                                                   TACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_pae_tpiA/1978368‑1978648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑