Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	27 [0]	[0] 27	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	26 (0.930)	24/274	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

GGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAA  >  NC_000913_3_pae_tpiA/1979144‑1979424                                                                                                                                        |                                                                                                                                                  tacacttatctaaggtttttcctaaatcgacggaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacagaaaaccctcaagtacttaacataaatgTATAAGTCATACtt                                                                                                                                      <  2:47789‑M2/14‑1 (MQ=255)  acacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttt                                                                                                                                     >  1:265343‑M2/135‑149 (MQ=255)         tctaagattttgcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacaaaaaacactcaattacttaacataaatgTATAAGTCATACttttgttttg                                                                                                                              <  2:218203‑M2/22‑1 (MQ=255)                      ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                 >  2:486094‑M2/115‑149 (MQ=255)                        aaaccgacgcaactgtacccgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCt                                                                                                               <  1:456651‑M2/37‑1 (MQ=255)                                  aactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGt                                                                                                     >  1:419055‑M2/103‑149 (MQ=255)                                         ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcaaacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTtcgg                                                                                              >  1:310735‑M2/96‑148 (MQ=255)                                           cgtcactacacccacatacaaccgagggtggctcccactttcaataatgcgtgatgcagatcacacaaaacactcaattacctaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgcta                                                                                            <  2:186785‑M2/56‑1 (MQ=255)                                            gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCATTCTGTTAAAAAGTTTtttgcttc                                                                                           >  1:271972‑M2/93‑147 (MQ=255)                                            gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAGGTTTTTcgctac                                                                                           >  1:271980‑M2/93‑149 (MQ=255)                                             tcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagataaaacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTAAATCTGTTAAAAAGGTTTTcgttacg                                                                                          >  1:68181‑M2/92‑149 (MQ=255)                                              cactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgttacgc                                                                                         <  2:453586‑M2/59‑1 (MQ=255)                                                    acgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacacgcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTGTCGCTACGCTagcaa                                                                                   >  2:359465‑M2/85‑149 (MQ=255)                                                        acatacaacggaggggggctgcgattttcaataatgcgtgctgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATGCTTTTGTTTTGGGTGTATTTCAATGTGTTAGAAAGTTTTTCGCTAGGCGCgcaagcaa                                                                               >  1:257518‑M2/81‑149 (MQ=255)                                                           tacaacggatggtggctgcgaatttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGGCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                                                                            <  2:257518‑M2/72‑1 (MQ=255)                                                                          ctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat                                                             >  1:32043‑M2/63‑149 (MQ=255)                                                                              gattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGaa                                                         >  1:306120‑M2/59‑149 (MQ=255)                                                                               attttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGaaa                                                        >  1:114286‑M2/58‑149 (MQ=255)                                                                                   tcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATTAAACAGGAATAATCGAAATtgg                                                    >  2:426390‑M2/54‑149 (MQ=255)                                                                                                           acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAATAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATCACt                            >  2:326738‑M2/30‑149 (MQ=255)                                                                                                           acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCAAAGACAAAATAACt                            >  2:361604‑M2/30‑149 (MQ=255)                                                                                                                    tccattacttaacataaattTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGTGCACAGTCAAAATAACTCACCGTaaa                   <  2:512579‑M2/129‑1 (MQ=255)                                                                                                                    tcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTAAAAATaactcacactatg                   >  2:259974‑M2/21‑143 (MQ=255)                                                                                                                       attacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaataa                >  1:516813‑M2/18‑149 (MQ=255)                                                                                                                           cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCTCAGTCAAAATAACTCACCGTAAATAatcat            >  2:173182‑M2/14‑149 (MQ=255)                                                                                                                              aacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTg         <  2:22825‑M2/139‑1 (MQ=255)                                                                                                                                     atgCATAAGTCATACTTTTGTTTTGTGTGTACTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAAGAACCAGGAATAACCGAAATGGGATGTTGCGCCCAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaa  <  2:51743‑M2/146‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                  GGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAA  >  NC_000913_3_pae_tpiA/1979144‑1979424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑