New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	4082549 =	52 (1.300)	5 (0.130)	5/260	4.7	8.8%	coding (211/903 nt)	fdoH	formate dehydrogenase‑O, Fe‑S subunit
	?	NC_000913_3_pae_tpiA	4082575 =	55 (1.470)					coding (185/903 nt)	fdoH	formate dehydrogenase‑O, Fe‑S subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AATAATTGCACCTTCCGCCGGGCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCG  >  NC_000913_3_pae_tpiA/4082403‑4082692                                                                                                                                                   |                                                                                                                                                aataatTGCACCTTCCGCCGGGAACGTTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCAt                                                                                                                                               <  1:546909/149‑1 (MQ=255)      ttGCACCTTCCGCCGGGCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCCTTgtgg                                                                                                                                          >  1:263/1‑149 (MQ=255)            cTTCCGCCGGGCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCg                                                                                                                                    >  2:523439/1‑149 (MQ=255)                cgccgGGCACGCTTTCAGGCAGGCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTaca                                                                                                                                <  2:254415/149‑1 (MQ=255)                          gCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGtt                                                                                                                      >  1:466220/1‑149 (MQ=255)                          gCTTTCAGGCAGCCTGGAGCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGtt                                                                                                                      <  1:323195/149‑1 (MQ=255)                             ttCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGAGATCGGACGATGGGGGTTGTCGTACACCCCAATGTTAtt                                                                                                                   <  2:512794/149‑1 (MQ=38)                               cAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGCCGTACACCCCAATGTTATTGc                                                                                                                 <  2:626613/149‑1 (MQ=255)                                  gCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTAAACCACAATGTTATTGCCGa                                                                                                              >  1:452401/1‑149 (MQ=255)                                    aGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACg                                                                                                            <  1:652099/149‑1 (MQ=255)                                      ccTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGt                                                                                                          >  1:469913/1‑149 (MQ=255)                                        tGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCGATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTAt                                                                                                        <  1:29498/149‑1 (MQ=255)                                           aTCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCTACGGAATcgc                                                                                                     >  2:467626/1‑149 (MQ=255)                                            acGGAACAGTTCTTACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGAATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCATTGTTATTGCCCACGCTATcgcg                                                                                                    >  2:515261/2‑149 (MQ=255)                                                     tGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGtt                                                                                           <  1:240003/149‑1 (MQ=255)                                                       cATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATGATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTcc                                                                                         >  1:700466/1‑149 (MQ=255)                                                       cATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTcc                                                                                         >  1:699907/1‑149 (MQ=255)                                                          aCAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAACGTTATTGCCGACGGTATCGCGAATGTCTTTCCAct                                                                                      >  2:417124/1‑149 (MQ=255)                                                            aGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGCTTTGGCGCTTAAATCATTGGGGTTGTCGTCCACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCActct                                                                                    >  1:19401/1‑149 (MQ=255)                                                                 tCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTacac                                                                                                                               <  1:96146/101‑1 (MQ=255)                                                                 tCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTacac                                                                                                                               >  2:96146/1‑101 (MQ=255)                                                                 tCTTTGCGAATCAGCCATTGCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTGAATCATTGGGGTGATGGTACACCCAAATGTTATTGCCGACGGTGTAGAGAATATCGTTCCAATCTGcaca                                                                               >  2:601701/1‑149 (MQ=255)                                                                   tttGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCCCCGCTATCGCGAATGTCGTTTCACTCTGcacaag                                                                             >  2:667612/1‑147 (MQ=255)                                                                         gATCGGCCATTCCATTTTGTCGTTCTGCTCCCCTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCGAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCt                                                                       <  1:467626/149‑1 (MQ=255)                                                                              gCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAGTTTCGTTCCACTCTGAACCCGCCACCTGCCAg                                                                  >  1:63284/1‑149 (MQ=255)                                                                                  ttCTAGTTTGTCGTTCGGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTTGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCtt                                                              <  2:209767/149‑1 (MQ=255)                                                                                  ttCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCTTTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCtt                                                              >  1:283824/1‑149 (MQ=255)                                                                                              gTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGGTGTCGTACACCCCAATGTTATTGCCGACGGTACCCCGAATGTCGTTCAACTCTGAACACGCCACCTGACAGACTTTACAGCCGacac                                                  >  2:55592/1‑149 (MQ=255)                                                                                                tCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAg                                                <  2:32692/149‑1 (MQ=255)                                                                                                  tGCCCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAggt                                              <  2:285248/149‑1 (MQ=255)                                                                                                       cACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCCTTGGGGTTGTCGTACACCCCCATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACCCGCCACATGACAGGCTTTACAGCCGAAACAGGTGGCaa                                         >  2:217305/1‑149 (MQ=255)                                                                                                       cACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTTTCGTACACCCCAATGTTATTCCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGACTTTACAGCCGGTACAagtgtgaa                                         >  2:223252/1‑145 (MQ=255)                                                                                                       cACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTaa                                         >  1:570510/1‑149 (MQ=255)                                                                                                          ttCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCCCTGTTATTACCGACGGTATCGCGAATGTCGTTCCACTCTGCACACGCCACCTGACAGGCTTTCCAGCCGCTACAGGTGGTAACGt                                      >  1:281142/1‑149 (MQ=255)                                                                                                          ttCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGt                                      >  2:555500/1‑149 (MQ=255)                                                                                                             cGAGAATCGCATTATCTTGCATGATTTGGCGTTTAAATCACTGGAGTAGCAGTACTTTCAAATGATATTGCCGACGGTATCGCGAATGTCGTTTCACGCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGa                                   <  1:277487/149‑1 (MQ=18)                                                                                                             cGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGa                                   >  1:450913/1‑149 (MQ=255)                                                                                                             cGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGa                                   <  2:570510/149‑1 (MQ=255)                                                                                                              gagaAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATAACGGTGGTAACCTCAAt                                  >  2:413040/1‑149 (MQ=255)                                                                                                                   gcgcATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTCCAGCCGATACAGGTGGTAAGGTCGATGAGtt                             >  2:561066/1‑149 (MQ=255)                                                                                                                    cgcATTGCCGTCCACGATTTGGCGCTTAAATCATTGGGGTGGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGttt                            <  2:700466/149‑1 (MQ=255)                                                                                                                    cgcATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGttt                            <  2:699907/149‑1 (MQ=255)                                                                                                                       aTTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACTTCGATGAGTTTCGc                         >  1:477299/1‑149 (MQ=255)                                                                                                                            cGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCActt                    >  1:447144/1‑149 (MQ=255)                                                                                                                                 aCGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTcc               >  1:217755/1‑149 (MQ=255)                                                                                                                                 aCGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTcc               >  2:532388/1‑149 (MQ=255)                                                                                                                                  tgttttGGCGCCTAAATCATTGGTGTTGTCGTTCGCCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCCCTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACATCGATGAGTTTCGCCACTTCTTCCt              <  2:228498/146‑1 (MQ=37)                                                                                                                                   gATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTg             <  1:222704/149‑1 (MQ=255)                                                                                                                                      ttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTCATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGaa          <  1:707241/149‑1 (MQ=255)                                                                                                                                           gcTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGGTTCGCCACTTCTTCCTGGAAATccc     >  1:76828/1‑149 (MQ=255)                                                                                                                                             ttAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCcgc   <  1:532388/149‑1 (MQ=255)                                                                                                                                              tAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCcgcg  >  2:444667/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                AATAATTGCACCTTCCGCCGGGCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCG  >  NC_000913_3_pae_tpiA/4082403‑4082692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.