breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 275,065 G→A T18I (ACC→ATC)  insH1 ← IS5 transposase and trans‑activator
RA 349,615 T→C intergenic (+43/‑397) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 349,624 C→T intergenic (+52/‑388) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 349,642 A→G intergenic (+70/‑370) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 349,673 G→C intergenic (+101/‑339) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 349,676 G→A intergenic (+104/‑336) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter
RA 1,756,356 A→C K220T (AAA→ACA)  pykF → pyruvate kinase I
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,106,741 A→T S162S (TCT→TCA wbbH ← O‑antigen polymerase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter
RA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase
RA 3,440,923 C→T G36D (GGC→GAC)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,111,596 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein
RA 4,158,703 A→G T100A (ACA→GCA)  oxyR → oxidative and nitrosative stress transcriptional regulator
RA 4,296,297 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,604,632 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 3423739–3424234 3424592–3424238 5–854 80 [72] [77] 82 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 1207790 =24 (0.170)89 (0.710) 75/234 0.9 80.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_tpiA 1209619 = 22 (0.170)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_tpiA = 120780525 (0.170)117 (0.930) 86/234 0.4 84.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_tpiA = 1209602 22 (0.170)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)168 (1.210) 116/258 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein