New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 4385264 =58 (0.880)4 (0.070) 4/260 8.9 6.5% intergenic (+7/‑45) yjeM/yjeN putative transporter/uncharacterized protein
?NC_000913_3_pae_tpiA 4385291 = 61 (1.010)intergenic (+34/‑18) yjeM/yjeN putative transporter/uncharacterized protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATT  >  NC_000913_3_pae_tpiA/4385120‑4385404
                                                                                                                                                |                                                                                                                                            
ccTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCt                                                                                                                                          >  1:586123/1‑149 (MQ=255)
  tgtgGTTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCtct                                                                                                                                        >  2:25099/1‑147 (MQ=255)
      gTTGAAGCCGGAGAGTGGGACAGCCCATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTc                                                                                                                                    >  1:956505/1‑149 (MQ=255)
      gTTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTc                                                                                                                                    >  1:166413/1‑149 (MQ=255)
        tGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGTTCTCTGGCAGCAGTGAAGTCGAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTa                                                                                                                                  <  1:539821/149‑1 (MQ=255)
              cGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGAc                                                                                                                            >  1:1045081/1‑149 (MQ=255)
                   aCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAg                                                                                                                       <  2:586123/149‑1 (MQ=255)
                    gtGGGAAAGCACATTGTGGATGATTGGCGGCCCTGGCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAgg                                                                                                                      <  2:55709/148‑1 (MQ=255)
                    cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAgg                                                                                                                      <  2:845186/149‑1 (MQ=255)
                       ggACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCg                                                                                                                   >  1:743311/1‑149 (MQ=255)
                       ggACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACCATAGGGCg                                                                                                                   >  2:314697/1‑149 (MQ=255)
                          caccacaTTGTGGATGGTTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGTGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTaa                                                                                                                <  2:202528/146‑1 (MQ=255)
                            gCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGa                                                                                                                             <  2:1091012/134‑1 (MQ=255)
                            gCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGa                                                                                                                             >  1:1091012/1‑134 (MQ=255)
                              acaTTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGTGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGAc                                                                                                            >  2:222877/1‑149 (MQ=255)
                                    tgGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGaaa                                                                                                      >  1:291251/1‑149 (MQ=255)
                                          gttGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTAGTGCAGCAGAGTGGCAAAAAAGCCGTAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTAt                                                                                                <  2:571421/148‑1 (MQ=255)
                                              gtggtcttGTTTTTTTTTCGCTGTTAGTGATTGCGTTTTTCCAGAAGTATTGCAGCAGAGTGGCAAAAATTTCGCAGTGGGCGGTTGAATAACGTGGTTCTCTATCGTTTACCTGACGATAGGGCGTAAAGTCATGAAATGTTATGGAt                                                                                            <  2:1079551/142‑1 (MQ=255)
                                              gcggcCCTGTCTTCTTCTCGCTGTTAGGGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGAt                                                                                            <  2:801782/149‑1 (MQ=255)
                                                 gcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGac                                                                                         >  2:761799/1‑149 (MQ=255)
                                                   ccTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGacac                                                                                       <  2:815813/149‑1 (MQ=255)
                                                   ccTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGacac                                                                                       >  1:123990/1‑149 (MQ=255)
                                                      gTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAg                                                                                    >  1:453457/1‑149 (MQ=255)
                                                          tcttctTGGTGTTAGCGATGGCCATTTTCCAGAAATATGGCAGCAGAGTGGCAAAAAATCCCCAGTGGGCGGTGGAATAAACCGCTGCCCTATCGGCTACCTGGCGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCgg                                                                                <  1:960646/149‑1 (MQ=255)
                                                          tctcgtcGCTGTTCGCGATGGCGATTTACCAGAAATATTGGAGAAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGGTGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCgg                                                                                <  2:267458/144‑1 (MQ=255)
                                                            ttctCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGa                                                                              <  2:810082/149‑1 (MQ=255)
                                                            tgctggCTGTTAGCTATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCTGTGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGa                                                                              <  2:806626/144‑1 (MQ=255)
                                                              ctcGCTGTTAGCGATGGCGATCTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAAt                                                                                                     >  1:401557/1‑124 (MQ=255)
                                                              ctcGCTGTTAGCGATGGCGATCTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAAt                                                                                                     <  2:401557/124‑1 (MQ=255)
                                                               tcGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGAccc                                                                           <  2:743311/149‑1 (MQ=255)
                                                                        gtgATGGCGGTTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAc                                                                  <  2:104953/147‑1 (MQ=255)
                                                                            tGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGaa                                                              >  1:131936/1‑149 (MQ=255)
                                                                             ggCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAg                                                             >  1:160895/1‑149 (MQ=255)
                                                                                gATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGacac                                                                                       >  2:219294/1‑120 (MQ=255)
                                                                                gATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGacac                                                                                       <  1:219294/120‑1 (MQ=255)
                                                                                gATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATg                                                          >  1:1025464/1‑149 (MQ=255)
                                                                                      ccAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCa                                                    >  1:408639/1‑149 (MQ=255)
                                                                                       tacgaCTATAGCAGGAGAGCTGCAAAAAAGCCGCAGGGGGCGGGGGAATAACCCGATTCCCTATTGTCTACCTGACGATAGGGCGTAAAGAGAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAg                                                   <  2:887950/145‑1 (MQ=255)
                                                                                        agcaCTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTCGAATAACCCGATGCCATATCGTCTCCCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAgg                                                  <  1:706457/146‑1 (MQ=255)
                                                                                        aGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACGGGAAATGTTATGGAGGACACCAGCCGGGACCCGGCAATAACCGAAGATGAATTCAgg                                                  >  2:191168/1‑149 (MQ=255)
                                                                                        aGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAgg                                                  <  2:160895/149‑1 (MQ=255)
                                                                                        aGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCATCTACGGTACGATAGGGCGTAAAGACAGGACTTGTTATCGATGACACCAGCCGGGACCCGGCACTAACCGAAGATGAAAGCAgg                                                  >  2:63019/1‑149 (MQ=255)
                                                                                                     cagAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAAtttt                                     <  2:291251/149‑1 (MQ=255)
                                                                                                       gagTGGCAAAAAATCCCCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCt                                   >  1:965469/1‑149 (MQ=255)
                                                                                                          tGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGAGCTGCACTTTTCTGcc                                >  2:644691/1‑149 (MQ=255)
                                                                                                          tGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAAACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAACAACCGCAGACCAAATCAGGGCGCTCCAAtttcaccct                                >  2:1060543/1‑143 (MQ=255)
                                                                                                           ggCAAAAAATCCGGAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCg                               <  2:580272/149‑1 (MQ=255)
                                                                                                            gCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGCAGATGAAATCAGGGCGATGCAATTTTCTGCCgt                              >  2:215636/1‑148 (MQ=255)
                                                                                                              aaaaaaTCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGAGCGCTGCAATTTTCTGCCgggg                            >  1:382483/1‑149 (MQ=255)
                                                                                                                          gTGGGCGGTGGAATAACCCGATGCCCCATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGaaa                <  1:13776/149‑1 (MQ=255)
                                                                                                                                   ggAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGa       <  1:391464/149‑1 (MQ=255)
                                                                                                                                   ggAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGa       <  1:222877/149‑1 (MQ=255)
                                                                                                                                     aaTAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGAc      >  1:964844/1‑148 (MQ=255)
                                                                                                                                     aaTAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGAc      <  2:964844/148‑1 (MQ=255)
                                                                                                                                     aaTAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACg     >  1:76678/1‑149 (MQ=255)
                                                                                                                                      aTAACCCGATGCCCCATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGa    <  1:100936/149‑1 (MQ=255)
                                                                                                                                        aaCCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGGCGAtt  >  1:184912/1‑149 (MQ=255)
                                                                                                                                        aaCCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGAtt  <  1:39922/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
CCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATT  >  NC_000913_3_pae_tpiA/4385120‑4385404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.