Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,362,060 Δ228,509 bp [puuR]yneL 223 genes

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1362060 1590568 228509 61 [0] [0] 61 [puuR]–yneL [puuR],puuC,puuB,puuE,pspF,pspA,pspB,pspC,pspD,pspE,ycjM,ycjN,ycjO,ycjP,ycjQ,ycjR,ycjS,ycjT,ycjU,ycjV,ycjV,ompG,ycjW,ycjX,ycjF,tyrR,tpx,ycjG,mpaA,ymjC,ymjD,ycjY,pgrR,mppA,ynaI,insH1,ynaJ,uspE,fnr,ogt,abgT,abgB,abgA,abgR,mcaS,smrA,ydaM,ydaN,fnrS,dbpA,ttcA,intR,ydaQ,ydaC,ralR,recT,recE,racC,ydaE,kilR,sieB,ydaF,ydaG,racR,ydaS,ydaT,ydaU,ydaV,ydaW,rzpR,rzoR,trkG,ynaK,ydaY,tmpR,lomR,insH1,lomR,stfR,tfaR,pinR,ynaE,ttcC,uspF,ompN,micC,pfo,ydbJ,hslJ,ldhA,ydbH,ynbE,ydbL,feaR,feaB,tynA,paaZ,paaA,paaB,paaC,paaD,paaE,paaF,paaG,paaH,paaI,paaJ,paaK,paaX,paaY,ynbG,ydbA,insD1,insC1,ydbA,insI1,ydbA,ydbC,ydbD,ynbA,ynbB,ynbC,ynbD,azoR,hrpA,ydcF,aldA,gapC,gapC,cybB,rydC,ydcA,hokB,mokB,sokB,trg,ydcI,ydcJ,opgD,ydcH,rimL,ydcK,tehA,tehB,ydcL,insP,insQ,ydcO,ydcN,ydcP,yncJ,hicA,hicB,ydcR,ydcS,ydcT,ydcU,ydcV,patD,yncL,ydcX,ydcY,ydcZ,mnaT,curA,mcbR,yncD,yncE,ansP,yncG,yncH,rhsE,ydcD,yncI,yncI,ydcC,pptA,yddH,nhoA,yddE,narV,narW,narY,narZ,narU,yddK,yddK,yddL,yddG,fdnG,fdnH,fdnI,yddM,adhP,maeA,sra,bdm,osmC,ddpF,ddpD,ddpC,ddpB,ddpA,ddpX,dosP,dosC,yddW,gadC,gadB,pqqL,yddB,yddA,ydeM,ydeN,ydeO,safA,ydeP,ydeQ,ydeR,ydeS,ydeT,yneL,yneL

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 13620590 (0.000)61 (0.950) 47/276 0.3 100% coding (149/558 nt) puuR repressor for the divergent puu operons, putrescine inducible
?NC_000913_3_pae_tpiA 1590569 = 0 (0.000)intergenic (‑33/+285) yneL/hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase

GTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTCACTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTT  >  NC_000913_3_pae_tpiA/1361915‑1362204
                                                                                                                                                |                                                                                                                                                 
gTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcacc                                                                                                                                               <  2:1059585‑M1/149‑5 (MQ=255)
     gaGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctg                                                                                                                                          <  2:282872‑M1/149‑10 (MQ=255)
     gaGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACGCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctg                                                                                                                                          <  2:357004‑M1/149‑10 (MQ=255)
       gggACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggt                                                                                                                                        <  2:298426‑M1/149‑12 (MQ=255)
             ggCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttc                                                                                                                                  <  1:70401‑M1/149‑18 (MQ=255)
                gcCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgta                                                                                                                               >  1:927243‑M1/1‑129 (MQ=255)
                gcCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTATCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATAAGcaccctctggtgttttcgta                                                                                                                               >  1:674208‑M1/1‑129 (MQ=255)
                   aGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgc                                                                                                                            >  2:952995‑M1/1‑126 (MQ=255)
                        aaCGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGGTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgt                                                                                                                       >  1:74942‑M1/1‑121 (MQ=255)
                        aaCGCTTGGCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtga                                                                                                                       <  2:1021616‑M1/149‑29 (MQ=255)
                            cTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctat                                                                                                                   <  1:620536‑M1/149‑33 (MQ=255)
                                             agcagcagGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgct                                                                                                  <  1:191280‑M1/149‑50 (MQ=255)
                                                 gcagGGGCTTTCACAACGTCGGGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctccgatatgtcatcgtgtggttgctatgcgacagacactgcgctctta                                                                                              <  1:544086‑M1/149‑54 (MQ=255)
                                                     gggCTTTCACAACGTCGTGCCGCCGAACTCGCCGGGCTGACTCACCGTGCTATCAGTACGATAGAACAAGATAACGTCAGCCCTCCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcat                                                                                          <  1:975973‑M1/149‑58 (MQ=255)
                                                       gCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggt                                                                                                                                        >  1:352820‑M1/1‑90 (MQ=255)
                                                       gCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggt                                                                                                                                        <  2:352820‑M1/101‑12 (MQ=255)
                                                         tttCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatgggg                                                                                      <  1:961662‑M1/149‑62 (MQ=255)
                                                            cacaACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacaggcactgcgctcttagcatggggcag                                                                                   >  1:202122‑M1/1‑85 (MQ=255)
                                                             acaACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagg                                                                                  <  2:881839‑M1/149‑66 (MQ=255)
                                                                aCGTCGTGCCGCCGAACTCGCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaa                                                                               <  1:516847‑M1/149‑69 (MQ=255)
                                                                      tGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataacca                                                                         >  2:808036‑M1/1‑75 (MQ=255)
                                                                        ccgccgAATTCGCCGGGCTGACTCACAGTGCTATCCGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccagg                                                                       <  1:331002‑M1/149‑77 (MQ=255)
                                                                                 tctcCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctc                                                              >  1:887646‑M1/1‑64 (MQ=255)
                                                                                 tctcCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctc                                                              >  1:154501‑M1/1‑64 (MQ=255)
                                                                                 tctcCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctc                                                              >  1:953924‑M1/1‑64 (MQ=255)
                                                                                  ctcCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggattcacgaggctatttctcc                                                             >  2:315212‑M1/1‑63 (MQ=255)
                                                                                       ggatgacTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgca                                                        <  1:922916‑M1/146‑92 (MQ=255)
                                                                                        gCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggcataaccaggctatttctccatcccc                                                       >  1:315748‑M1/1‑57 (MQ=255)
                                                                                              tCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggcca                                                 >  1:85157‑M1/1‑51 (MQ=255)
                                                                                              tCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggcca                                                 >  1:447686‑M1/1‑51 (MQ=255)
                                                                                                acaGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaat                                               >  2:191220‑M1/1‑49 (MQ=255)
                                                                                                  aGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaatta                                             >  1:616894‑M1/1‑47 (MQ=255)
                                                                                                    tGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatg                                           <  1:910396‑M1/149‑105 (MQ=255)
                                                                                                      ccatcAGTACGATAGAACAAGACAACGTCCGCCCTGACATCAGcaccatctggggttttcgtatgcagtgtctatgcgacagactctgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaa                                         <  1:95385‑M1/147‑107 (MQ=255)
                                                                                                       tATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattctgaaa                                        >  2:396039‑M1/1‑42 (MQ=255)
                                                                                                       tATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaa                                        <  1:408387‑M1/149‑108 (MQ=255)
                                                                                                        aTCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaag                                       >  2:27018‑M1/1‑41 (MQ=255)
                                                                                                        aTCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcat                                                                                          >  1:1036353‑M1/1‑41 (MQ=255)
                                                                                                        aTCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcat                                                                                          <  2:1036353‑M1/98‑58 (MQ=255)
                                                                                                                 aTAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggc                              <  2:447686‑M1/149‑118 (MQ=255)
                                                                                                                  tAGAACAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggct                             >  2:918392‑M1/1‑31 (MQ=255)
                                                                                                                   aaaaccAGGTAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttacaatcggtgaggccaattatgaaagaggagaggctt                            <  2:793927‑M1/147‑120 (MQ=255)
                                                                                                                      tcaAGATAAAGTCAGCCCGGCCGCCAGcaccctcttgtgttttcgtaggcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaag                         <  2:154501‑M1/148‑123 (MQ=255)
                                                                                                                      aCAAGATAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaag                         >  1:407196‑M1/1‑27 (MQ=255)
                                                                                                                       cAAGATAAAGTCAGCCCTGCCATCAGccccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagtggagaggcttaagt                        >  2:1088886‑M1/1‑26 (MQ=255)
                                                                                                                           aTAAAGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatacagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaagttctt                    >  2:859939‑M1/1‑22 (MQ=255)
                                                                                                                               aGTCAGCCCTTCCAGCAGcaccctttggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccatgctattgctccatgcataggccaattatgaaagaggagaggcttaagttcttgaac                <  2:453910‑M1/149‑132 (MQ=255)
                                                                                                                               aGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatga                                          >  1:91975‑M1/1‑18 (MQ=255)
                                                                                                                               aGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatga                                          <  2:91975‑M1/123‑106 (MQ=255)
                                                                                                                               aGTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggagcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaagttcttgaac                <  1:764986‑M1/149‑132 (MQ=255)
                                                                                                                                gTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgagaggggagaggcttaagttcttgaacc               >  2:367592‑M1/1‑17 (MQ=255)
                                                                                                                                gTCAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaagttcttgaacc               >  2:986487‑M1/1‑17 (MQ=255)
                                                                                                                                 tCAGCCCTGCCATCAGccccctctggtggtttcgtatgcagtgtctatgcgactgacactgcgctcttagcatggggctgggaataaccaggctatttctccgtacacaggccaattatgaaagaggagaggcttaagttcttgaaccc              >  2:620111‑M1/1‑16 (MQ=255)
                                                                                                                                  cAGCCCTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctattagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaagttcttgaaccct             <  2:887646‑M1/149‑135 (MQ=255)
                                                                                                                                     cccTGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcccaggccaactctgaaagaggagcgccttaagtccttgaaacctgaa          >  2:624920‑M1/1‑12 (MQ=255)
                                                                                                                                        tGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaa                                        <  2:77449‑M1/116‑108 (MQ=255)
                                                                                                                                        tGCCATCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaa                                        >  1:77449‑M1/1‑9 (MQ=255)
                                                                                                                                            aTCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaagttcttgaaccctgaacgaataa   >  1:1082927‑M1/1‑5 (MQ=255)
                                                                                                                                            aTCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggcaaattatgaaagaggagaggcttaagttcttgaaccctgaacgaataa   >  1:583671‑M1/1‑5 (MQ=255)
                                                                                                                                            aTCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatatctccatgcacaggccaattatgaaggaggagaggcttaagttcttgaaccctggacgaataa   >  1:980507‑M1/1‑5 (MQ=255)
                                                                                                                                             tCAGcaccctctggtgttttcgtatgcagtgtctatgcgacagacactgcgctcttagcatggggcagggaataaccaggctatttctccatgcacaggccaattatgaaagaggagaggcttaagttcttgaaccctgaacgaataac  >  1:744544‑M1/1‑4 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAAAGCTGCTGAAGGTGTATGGTCTGTCACTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATTAATCAGGACGACTTAATTGAGATGGGTAGTCAGGGTGTGTCAATGAAGCTGGTT  >  NC_000913_3_pae_tpiA/1361915‑1362204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: