Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,362,060 Δ228,509 bp [puuR]yneL 223 genes

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1362060 1590568 228509 61 [0] [0] 61 [puuR]–yneL [puuR],puuC,puuB,puuE,pspF,pspA,pspB,pspC,pspD,pspE,ycjM,ycjN,ycjO,ycjP,ycjQ,ycjR,ycjS,ycjT,ycjU,ycjV,ycjV,ompG,ycjW,ycjX,ycjF,tyrR,tpx,ycjG,mpaA,ymjC,ymjD,ycjY,pgrR,mppA,ynaI,insH1,ynaJ,uspE,fnr,ogt,abgT,abgB,abgA,abgR,mcaS,smrA,ydaM,ydaN,fnrS,dbpA,ttcA,intR,ydaQ,ydaC,ralR,recT,recE,racC,ydaE,kilR,sieB,ydaF,ydaG,racR,ydaS,ydaT,ydaU,ydaV,ydaW,rzpR,rzoR,trkG,ynaK,ydaY,tmpR,lomR,insH1,lomR,stfR,tfaR,pinR,ynaE,ttcC,uspF,ompN,micC,pfo,ydbJ,hslJ,ldhA,ydbH,ynbE,ydbL,feaR,feaB,tynA,paaZ,paaA,paaB,paaC,paaD,paaE,paaF,paaG,paaH,paaI,paaJ,paaK,paaX,paaY,ynbG,ydbA,insD1,insC1,ydbA,insI1,ydbA,ydbC,ydbD,ynbA,ynbB,ynbC,ynbD,azoR,hrpA,ydcF,aldA,gapC,gapC,cybB,rydC,ydcA,hokB,mokB,sokB,trg,ydcI,ydcJ,opgD,ydcH,rimL,ydcK,tehA,tehB,ydcL,insP,insQ,ydcO,ydcN,ydcP,yncJ,hicA,hicB,ydcR,ydcS,ydcT,ydcU,ydcV,patD,yncL,ydcX,ydcY,ydcZ,mnaT,curA,mcbR,yncD,yncE,ansP,yncG,yncH,rhsE,ydcD,yncI,yncI,ydcC,pptA,yddH,nhoA,yddE,narV,narW,narY,narZ,narU,yddK,yddK,yddL,yddG,fdnG,fdnH,fdnI,yddM,adhP,maeA,sra,bdm,osmC,ddpF,ddpD,ddpC,ddpB,ddpA,ddpX,dosP,dosC,yddW,gadC,gadB,pqqL,yddB,yddA,ydeM,ydeN,ydeO,safA,ydeP,ydeQ,ydeR,ydeS,ydeT,yneL,yneL

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 13620590 (0.000)61 (0.950) 47/276 0.3 100% coding (149/558 nt) puuR repressor for the divergent puu operons, putrescine inducible
?NC_000913_3_pae_tpiA 1590569 = 0 (0.000)intergenic (‑33/+285) yneL/hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase

TAGCTTTCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAAC  >  NC_000913_3_pae_tpiA/1590424‑1590713
                                                                                                                                                 |                                                                                                                                                   
gtgatgagggactggcgccaggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCAcc                                                                                                                                                  <  2:1059585‑M2/4‑1 (MQ=255)
     gagggactggcgccaggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTg                                                                                                                                             <  2:282872‑M2/9‑1 (MQ=255)
     gagggactggcgccaggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgacgcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTg                                                                                                                                             <  2:357004‑M2/9‑1 (MQ=255)
       gggactggcgccaggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGgt                                                                                                                                           <  2:298426‑M2/11‑1 (MQ=255)
             ggcgccaggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑Tc                                                                                                                                   <  1:70401‑M2/17‑1 (MQ=255)
                gccaggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTa                                                                                                                                >  1:927243‑M2/130‑149 (MQ=255)
                gccaggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactatccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccataagCACCCTCTGGTGTTT‑‑TCGTa                                                                                                                                >  1:674208‑M2/130‑149 (MQ=255)
                   aggaaaacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGc                                                                                                                             >  2:952995‑M2/127‑149 (MQ=255)
                        aacgcttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtggtatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAgtgt                                                                                                                        >  1:74942‑M2/122‑149 (MQ=255)
                        aacgcttggcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCagtga                                                                                                                        <  2:1021616‑M2/28‑2 (MQ=255)
                            cttgtcggaaatccgccagcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTAt                                                                                                                    <  1:620536‑M2/32‑1 (MQ=255)
                                             agcagcaggggctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGct                                                                                                   <  1:191280‑M2/49‑1 (MQ=255)
                                                 gcaggggctttcacaacgtcgggccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCCGATATGTCATCGTGTG‑‑GTTGCTATGCGACAGACACTGCGCTCTTa                                                                                               <  1:544086‑M2/53‑1 (MQ=255)
                                                     gggctttcacaacgtcgtgccgccgaactcgccgggctgactcaccgtgctatcagtacgatagaacaagataacgtcagccctcccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCAt                                                                                           <  1:975973‑M2/57‑1 (MQ=255)
                                                       gctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGgt                                                                                                                                           >  1:352820‑M2/91‑101 (MQ=255)
                                                       gctttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGgt                                                                                                                                           <  2:352820‑M2/11‑1 (MQ=255)
                                                         tttcacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATgggg                                                                                       <  1:961662‑M2/61‑1 (MQ=255)
                                                            cacaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGGCACTGCGCTCTTAGCATGGGGCAg                                                                                    >  1:202122‑M2/86‑149 (MQ=255)
                                                             acaacgtcgtgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAgg                                                                                   <  2:881839‑M2/65‑1 (MQ=255)
                                                                acgtcgtgccgccgaactcgccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGaa                                                                                <  1:516847‑M2/68‑1 (MQ=255)
                                                                      tgccgccgaactctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCa                                                                          >  2:808036‑M2/76‑149 (MQ=255)
                                                                        ccgccgaattcgccgggctgactcacagtgctatccgtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAgg                                                                        <  1:331002‑M2/76‑1 (MQ=255)
                                                                                 tctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtctc                                                               >  1:887646‑M2/65‑149 (MQ=255)
                                                                                 tctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtctc                                                               >  1:953924‑M2/65‑149 (MQ=255)
                                                                                 tctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtctc                                                               >  1:154501‑M2/65‑149 (MQ=255)
                                                                                  ctccgggctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGATTCACGAGGCTATTTCTcc                                                              >  2:315212‑M2/64‑149 (MQ=255)
                                                                                       ggatgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGca                                                        <  1:922916‑M2/91‑1 (MQ=255)
                                                                                        gctgactcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGCATAACCAGGCTATTTCtccatcccc                                                        >  1:315748‑M2/58‑145 (MQ=255)
                                                                                              tcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCa                                                 >  1:85157‑M2/52‑149 (MQ=255)
                                                                                              tcacagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCa                                                 >  1:447686‑M2/52‑149 (MQ=255)
                                                                                                acagtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAAt                                               >  2:191220‑M2/50‑149 (MQ=255)
                                                                                                  agtgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTa                                             >  1:616894‑M2/48‑149 (MQ=255)
                                                                                                    tgctatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATg                                           <  1:910396‑M2/104‑1 (MQ=255)
                                                                                                      ccatcagtacgatagaacaagacaacgtccgccctgacatcagCACCATCTGGGGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACTCTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa                                         <  1:95385‑M2/106‑1 (MQ=255)
                                                                                                       tatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTCTGaaa                                        >  2:396039‑M2/43‑149 (MQ=255)
                                                                                                       tatcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaaa                                        <  1:408387‑M2/107‑1 (MQ=255)
                                                                                                        atcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCAt                                                                                           >  1:1036353‑M2/42‑98 (MQ=255)
                                                                                                        atcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCAt                                                                                           <  2:1036353‑M2/57‑1 (MQ=255)
                                                                                                        atcagtacgatagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAag                                       >  2:27018‑M2/42‑149 (MQ=255)
                                                                                                                 atagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGc                              <  2:447686‑M2/117‑1 (MQ=255)
                                                                                                                  tagaacaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCt                             >  2:918392‑M2/32‑149 (MQ=255)
                                                                                                                   aaaaccaggtaaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTACAATCGGTGAGGCCAATTATGAAAGAGGAGAGGCtt                            <  2:793927‑M2/119‑1 (MQ=255)
                                                                                                                      tcaagataaagtcagcccggccgccagCACCCTCTTGTGTTT‑‑TCGTAGGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAg                         <  2:154501‑M2/122‑1 (MQ=255)
                                                                                                                      acaagataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAg                         >  1:407196‑M2/28‑149 (MQ=255)
                                                                                                                       caagataaagtcagccctgccatcagCCCCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGTGGAGAGGCTTAAGt                        >  2:1088886‑M2/27‑149 (MQ=255)
                                                                                                                           ataaagtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATACAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCtt                    >  2:859939‑M2/23‑149 (MQ=255)
                                                                                                                               agtcagcccttccagcagCACCCTTTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCATGCTATTGCTCCATGCATAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAAc                <  2:453910‑M2/131‑1 (MQ=255)
                                                                                                                               agtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGa                                          >  1:91975‑M2/19‑123 (MQ=255)
                                                                                                                               agtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGa                                          <  2:91975‑M2/105‑1 (MQ=255)
                                                                                                                               agtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGAGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAAc                <  1:764986‑M2/131‑1 (MQ=255)
                                                                                                                                gtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAGAGGGGAGAGGCTTAAGTTCTTGAAcc               >  2:367592‑M2/18‑149 (MQ=255)
                                                                                                                                gtcagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAAcc               >  2:986487‑M2/18‑149 (MQ=255)
                                                                                                                                 tcagccctgccatcagCCCCCTCTGGTGGTT‑‑TCGTATGCAGTGTCTATGCGACTGACACTGCGCTCTTAGCATGGGGCTGGGAATAACCAGGCTATTTCTCCGTACACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAAccc              >  2:620111‑M2/17‑149 (MQ=255)
                                                                                                                                  cagccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTATTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCt             <  2:887646‑M2/134‑1 (MQ=255)
                                                                                                                                     ccctgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCCCAGGCCAACTCTGAAAGAGGAGCGCCTTAAGTCCTTGAAACCTGaa          >  2:624920‑M2/13‑149 (MQ=255)
                                                                                                                                        tgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaaa                                        <  2:77449‑M2/107‑1 (MQ=255)
                                                                                                                                        tgccatcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaaa                                        >  1:77449‑M2/10‑116 (MQ=255)
                                                                                                                                            atcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATaa   >  1:1082927‑M2/6‑149 (MQ=255)
                                                                                                                                            atcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCAAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATaa   >  1:583671‑M2/6‑149 (MQ=255)
                                                                                                                                            atcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATATCTCCATGCACAGGCCAATTATGAAGGAGGAGAGGCTTAAGTTCTTGAACCCTGGACGAATaa   >  1:980507‑M2/6‑149 (MQ=255)
                                                                                                                                             tcagCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAAc  >  1:744544‑M2/5‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TAGCTTTCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTT‑‑TCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAAGAGGAGAGGCTTAAGTTCTTGAACCCTGAACGAATAAC  >  NC_000913_3_pae_tpiA/1590424‑1590713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: