New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 434171144 (1.130)4 (0.120) 4/250 4.8 9.4% intergenic (‑167/‑116) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
?NC_000913_3_pae_tpiA = 4341731 39 (1.150)intergenic (‑187/‑96) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGC  >  NC_000913_3_pae_tpiA/4341563‑4341859
                                                                                                                                                    |                                                                                                                                                    
gAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCCGAt                                                                                                                                                      >  1:295092/1‑149 (MQ=255)
    atCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTAc                                                                                                                                                  >  1:583115/1‑149 (MQ=255)
                  aGGGGTGAGGGAGGGTGACTGCGACTGTGATTACGTTTTGCACCCTTTTTCCTGATGGGCGAGGGGACTCTTCGAGTATCAGGAGGTCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATACACTCAGATTTACTGCTGTTTCATGCa                                                                                                                                    <  2:306667/149‑1 (MQ=255)
                          gggaggATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGCAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGa                                                                                                                            >  1:670238/1‑149 (MQ=255)
                             aggaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGt                                                                                                                          >  2:58288/1‑148 (MQ=255)
                             aggaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGt                                                                                                                          <  1:58288/148‑1 (MQ=255)
                                 tGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTTTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATg                                                                                                                     <  1:265476/149‑1 (MQ=255)
                                  gACTGCGAGTGGGAGCAAGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg                                                                                                                    <  1:128600/149‑1 (MQ=255)
                                    cTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTGACGCAGGATCTGAGTTTATGGGa                                                                                                                  >  1:231405/1‑149 (MQ=255)
                                            gcgAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCaa                                                                                                          <  2:294733/147‑1 (MQ=255)
                                                         tCACCCTCTTCCCAGAGGGGCGAGGGGATTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATTTGAGGTTATGGGAATGCTCAACCTGGAAGCCGGa                                                                                             <  2:214473/149‑1 (MQ=255)
                                                          cccccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAg                                                                                            <  2:390871/147‑1 (MQ=255)
                                                          cacaCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCGGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGACTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAg                                                                                            <  1:402288/149‑1 (MQ=255)
                                                             ccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCATATTTACTGCTGCTTCACGCAGGATCTGAGtt                                                                                                                         <  1:388712/117‑1 (MQ=255)
                                                             ccTCTTCCCAGAGGGGCGAGCGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCATATTTACTGCTGCTTCACGCAGGATCTGAGtt                                                                                                                         >  2:388712/1‑117 (MQ=255)
                                                                  tCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTg                                                                                    >  1:345868/1‑149 (MQ=255)
                                                                  tCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTg                                                                                    <  2:497084/149‑1 (MQ=255)
                                                                   cccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGc                                                                                   <  1:437575/149‑1 (MQ=255)
                                                                              gAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTAATGCGGTGACGGAGTTTATGGGAATGCTCAACCTGGAAGCCGGGGGCTTTCTGCAGATTCGCCTg                                                                        >  1:159746/1‑149 (MQ=255)
                                                                                  ggACTCTCCGAGTATCATGAGGCCGAAATCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCat                                                                    <  2:620007/149‑1 (MQ=255)
                                                                                   gACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTGTTCTGCAGATTCGccgtccagt                                                                   >  1:282221/1‑142 (MQ=255)
                                                                                      tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCatgatg                                                                >  1:508234/1‑149 (MQ=255)
                                                                                        tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGtt                                                                                         <  1:261569/122‑1 (MQ=255)
                                                                                        tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGtt                                                                                         >  2:261569/1‑122 (MQ=255)
                                                                                                tCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTca                                                      >  2:639178/1‑149 (MQ=255)
                                                                                                 cATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaa                                                     <  1:243919/149‑1 (MQ=255)
                                                                                                   tGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGTTCTTAGTTTATGGGAATGCTAAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagc                                                   >  2:453462/1‑149 (MQ=255)
                                                                                                   tGAGGCCGAAAACTATGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagc                                                   <  1:623331/149‑1 (MQ=255)
                                                                                                        ccGAAAACTCTGCTTTTAAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCTCCTGCCATGATGAAGTTATTCAAGCAAGcc                                              <  1:98990/149‑1 (MQ=255)
                                                                                                            agaCTCTGCTTTTGCGGTAATCTATTCCCATAAACTCATCTTTACTGCTGCTTCACGTAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCTGGTGGTTTTCTGGAGATTCGCCTGCCATGTTGAAGTTATTCAAGCAAGCCAGga                                          <  2:216666/147‑1 (MQ=255)
                                                                                                            aaaCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGga                                          >  1:152253/1‑149 (MQ=255)
                                                                                                                     ttttCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCAAGGAGAACTgaaa                                 >  1:61851/1‑146 (MQ=255)
                                                                                                                          aGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGt                            <  2:583115/149‑1 (MQ=255)
                                                                                                                          aGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGt                            <  2:670238/149‑1 (MQ=255)
                                                                                                                            gTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCt                          <  2:508234/149‑1 (MQ=255)
                                                                                                                                tttATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCAc                      <  1:639178/149‑1 (MQ=255)
                                                                                                                                    ttCCCATAAACTCAGATTTACTGCTGCTTCAAAAAAGATGAGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCaa                  >  1:674877/1‑149 (MQ=255)
                                                                                                                                      gctaTAAACTCAGATTTATTGGTGCTTCACGCAAGATCTGGTTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCaaaa                <  2:282221/146‑1 (MQ=255)
                                                                                                                                       ccATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTATTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGACTGTTGTCTACACCCAAAAt               >  2:611981/1‑149 (MQ=255)
                                                                                                                                       ccATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGACAGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTCCACCCaaact               >  2:44890/1‑147 (MQ=255)
                                                                                                                                        cATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCTAGGAGATCTGCATGATGTCTGCACCCAAAAtt              >  1:200870/1‑149 (MQ=255)
                                                                                                                                             aCTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCt                                                                                                             >  2:547503/1‑49 (MQ=255)
                                                                                                                                             aCTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCt                                                                                                             <  1:547503/49‑1 (MQ=255)
                                                                                                                                               tCAGATTTACTGCTGCTTCACGCCGGATCTGAGTTTACGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAACCAGGAGACCTGCACGATGTCTGCACCCAAAATTAAATTTa       >  2:80184/1‑149 (MQ=255)
                                                                                                                                                    tttACTGCTGCTTCACGCAGGATCGGAGTTCATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTGAAGCAAGCCCGGAGATCTGCATGCTGGATTCACACAAAATCACTTTTAACgac  >  2:644807/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGC  >  NC_000913_3_pae_tpiA/4341563‑4341859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.