New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4341711	44 (1.130)	4 (0.120)	4/250	4.8	9.4%	intergenic (‑167/‑116)	melR/melA	melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
	?	NC_000913_3_pae_tpiA	= 4341731	39 (1.150)					intergenic (‑187/‑96)	melR/melA	melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTT  >  NC_000913_3_pae_tpiA/4341589‑4341875                                                                                                                                               |                                                                                                                                                 gggaggATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGCAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGa                                                                                                                                            >  1:670238/1‑149 (MQ=255)    aggaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGt                                                                                                                                          >  2:58288/1‑148 (MQ=255)    aggaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGt                                                                                                                                          <  1:58288/148‑1 (MQ=255)        tGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTTTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATg                                                                                                                                     <  1:265476/149‑1 (MQ=255)         gACTGCGAGTGGGAGCAAGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg                                                                                                                                    <  1:128600/149‑1 (MQ=255)           cTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTGACGCAGGATCTGAGTTTATGGGa                                                                                                                                  >  1:231405/1‑149 (MQ=255)                   gcgAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCaa                                                                                                                          <  2:294733/147‑1 (MQ=255)                                tCACCCTCTTCCCAGAGGGGCGAGGGGATTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATTTGAGGTTATGGGAATGCTCAACCTGGAAGCCGGa                                                                                                             <  2:214473/149‑1 (MQ=255)                                 cccccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAg                                                                                                            <  2:390871/147‑1 (MQ=255)                                 cacaCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCGGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGACTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAg                                                                                                            <  1:402288/149‑1 (MQ=255)                                    ccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCATATTTACTGCTGCTTCACGCAGGATCTGAGtt                                                                                                                                         <  1:388712/117‑1 (MQ=255)                                    ccTCTTCCCAGAGGGGCGAGCGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCATATTTACTGCTGCTTCACGCAGGATCTGAGtt                                                                                                                                         >  2:388712/1‑117 (MQ=255)                                         tCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTg                                                                                                    >  1:345868/1‑149 (MQ=255)                                         tCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTg                                                                                                    <  2:497084/149‑1 (MQ=255)                                          cccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGc                                                                                                   <  1:437575/149‑1 (MQ=255)                                                     gAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTAATGCGGTGACGGAGTTTATGGGAATGCTCAACCTGGAAGCCGGGGGCTTTCTGCAGATTCGCCTg                                                                                        >  1:159746/1‑149 (MQ=255)                                                         ggACTCTCCGAGTATCATGAGGCCGAAATCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCat                                                                                    <  2:620007/149‑1 (MQ=255)                                                          gACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTGTTCTGCAGATTCGccgtccagt                                                                                   >  1:282221/1‑142 (MQ=255)                                                             tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCatgatg                                                                                >  1:508234/1‑149 (MQ=255)                                                               tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGtt                                                                                                         >  2:261569/1‑122 (MQ=255)                                                               tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGtt                                                                                                         <  1:261569/122‑1 (MQ=255)                                                                       tCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTca                                                                      >  2:639178/1‑149 (MQ=255)                                                                        cATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaa                                                                     <  1:243919/149‑1 (MQ=255)                                                                          tGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGTTCTTAGTTTATGGGAATGCTAAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagc                                                                   >  2:453462/1‑149 (MQ=255)                                                                          tGAGGCCGAAAACTATGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagc                                                                   <  1:623331/149‑1 (MQ=255)                                                                               ccGAAAACTCTGCTTTTAAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCTCCTGCCATGATGAAGTTATTCAAGCAAGcc                                                              <  1:98990/149‑1 (MQ=255)                                                                                   agaCTCTGCTTTTGCGGTAATCTATTCCCATAAACTCATCTTTACTGCTGCTTCACGTAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCTGGTGGTTTTCTGGAGATTCGCCTGCCATGTTGAAGTTATTCAAGCAAGCCAGga                                                          <  2:216666/147‑1 (MQ=255)                                                                                   aaaCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGga                                                          >  1:152253/1‑149 (MQ=255)                                                                                            ttttCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCAAGGAGAACTgaaa                                                 >  1:61851/1‑146 (MQ=255)                                                                                                 aGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGt                                            <  2:670238/149‑1 (MQ=255)                                                                                                 aGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGt                                            <  2:583115/149‑1 (MQ=255)                                                                                                   gTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCt                                          <  2:508234/149‑1 (MQ=255)                                                                                                       tttATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCAc                                      <  1:639178/149‑1 (MQ=255)                                                                                                           ttCCCATAAACTCAGATTTACTGCTGCTTCAAAAAAGATGAGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCaa                                  >  1:674877/1‑149 (MQ=255)                                                                                                             gctaTAAACTCAGATTTATTGGTGCTTCACGCAAGATCTGGTTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCaaaa                                <  2:282221/146‑1 (MQ=255)                                                                                                              ccATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTATTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGACTGTTGTCTACACCCAAAAt                               >  2:611981/1‑149 (MQ=255)                                                                                                              ccATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGACAGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTCCACCCaaact                               >  2:44890/1‑147 (MQ=255)                                                                                                               cATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCTAGGAGATCTGCATGATGTCTGCACCCAAAAtt                              >  1:200870/1‑149 (MQ=255)                                                                                                                    aCTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCt                                                                                                                             >  2:547503/1‑49 (MQ=255)                                                                                                                    aCTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCt                                                                                                                             <  1:547503/49‑1 (MQ=255)                                                                                                                      tCAGATTTACTGCTGCTTCACGCCGGATCTGAGTTTACGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAACCAGGAGACCTGCACGATGTCTGCACCCAAAATTAAATTTa                       >  2:80184/1‑149 (MQ=255)                                                                                                                           tttACTGCTGCTTCACGCAGGATCGGAGTTCATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTGAAGCAAGCCCGGAGATCTGCATGCTGGATTCACACAAAATCACTTTTAACgac                  >  2:644807/1‑147 (MQ=255)                                                                                                                                  ctgctTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATGACATTTATCGGCGCTGGtt           >  1:394984/1‑149 (MQ=255)                                                                                                                                    gctTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTcg         >  1:214517/1‑149 (MQ=255)                                                                                                                                    gctTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTcg         >  1:203224/1‑149 (MQ=255)                                                                                                                                     ctTCACGCAGGATCTGCGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGGTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCCGGTTcga        >  1:504646/1‑149 (MQ=255)                                                                                                                                           gCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGACGCTGGTTCGACGAttg  >  2:70038/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                 GGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTT  >  NC_000913_3_pae_tpiA/4341589‑4341875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.