New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 304599329 (1.170)4 (0.170) 4/274 2.8 12.9% intergenic (‑92/+175) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
?NC_000913_3_pae_tpiA = 3046006 26 (1.090)intergenic (‑105/+162) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Rejected: Frequency below/above cutoff threshold.

TGCAGTTGCCCGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGC  >  NC_000913_3_pae_tpiA/3045848‑3046139
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tGCAGTTGCCCGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGcc                                                                                                                                                 <  1:168073/149‑1 (MQ=255)
        cccgcccgATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAgg                                                                                                                                         >  1:383380/1‑149 (MQ=255)
          cgccgcaTGCCGCGACGGCCACCAGTCACAAGTGCTATAGCCATTTGTTTCTCTTCTCATAATTACTTTGTTGATGTAGCTTGGAGAGTGCTAACAAACTGGCTGTTTCTTCTATTTTGGTACGCTTTTCCCCCTCGCCCTTTCAGGga                                                                                                                                       <  2:335/143‑1 (MQ=255)
                  gCCCCGATTGCCACCAGTAACAGGTGCTATAGCCATTTGTTTTTACGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCg                                                                                                                               <  2:122864/149‑1 (MQ=255)
                    cgcgACTGCCACCGGGCAGAAGTGGTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCggg                                                                                                                             <  2:10552/149‑1 (MQ=255)
                    cgcgACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCtccctgaaagggcgagggggaaa                                                                                                                             >  1:208185‑M1/1‑126 (MQ=255)
                     gcgACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGCCGTATCTTTGGGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCGCGCCCTTTCCGGGCGGGGCCAgggg                                                                                                                            >  1:139010/1‑149 (MQ=255)
                                              ataGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGc                                                                                                   >  2:148252/1‑149 (MQ=255)
                                                  ccATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCtccctgaaagggcgagggggaaaagcgtgccaacattgaagattgagccagtt                                                                                               <  2:208185‑M1/149‑54 (MQ=255)
                                                  ccATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGc                                                                                               >  1:180908/1‑149 (MQ=255)
                                                     tttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTg                                                                                            >  2:330196/1‑149 (MQ=255)
                                                         tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTcc                                                                                        >  2:264671/1‑149 (MQ=255)
                                                         tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCATCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTcc                                                                                        >  2:373729/1‑149 (MQ=255)
                                                            ttCTGAGAAAAATTAATTCTTTGACGTATCTTTGAGATTGCTAACAAACTGGCTAAATCTTCAATGTTGGCACGCTTTTCCCCCTCGACTTTTCAGGGAGATGGCCGTGGTTAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCt                                                                                     <  2:114242/149‑1 (MQ=255)
                                                               cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAc                                                                                  >  1:26846/1‑149 (MQ=255)
                                                                gagaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGGGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                 >  1:53347/1‑149 (MQ=255)
                                                                               gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCtccctgaaagggcgagggggaaaa                                                                                                                            >  2:298097‑M1/1‑67 (MQ=255)
                                                                               gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCtccctgaaagggcgagggggaaaa                                                                                                                            <  1:298097‑M1/91‑25 (MQ=255)
                                                                                         cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                        <  1:148252/149‑1 (MQ=255)
                                                                                         cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGACGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTGTCCCCAAAGGGGCGAg                                                        <  1:367309/149‑1 (MQ=255)
                                                                                           ttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                      <  1:321461/149‑1 (MQ=255)
                                                                                           ttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                      >  2:214794/1‑149 (MQ=255)
                                                                                               gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACt                                                  >  2:184238/1‑149 (MQ=255)
                                                                                                aTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGAGGAGGGGAGTg                                                 >  1:142881/1‑149 (MQ=255)
                                                                                                   gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                              <  1:214794/149‑1 (MQ=255)
                                                                                                    cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACTCTAACCTTCTCCCCAAAGGGGCGAGGGGACTGTCTg                                             >  1:146300/1‑149 (MQ=255)
                                                                                                          aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaacgaattaatttttctcggaaaaacaaatgg                                       <  2:208185‑M2/149‑97 (MQ=255)
                                                                                                          aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                         >  2:112446/1‑99 (MQ=255)
                                                                                                          aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                         <  1:112446/99‑1 (MQ=255)
                                                                                                                cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTa                                 >  1:350296/1‑149 (MQ=255)
                                                                                                                           aTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                            <  1:269675/127‑1 (MQ=255)
                                                                                                                           aTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                            >  2:269675/1‑127 (MQ=255)
                                                                                                                                       ttttCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaac                                                                    <  1:298097‑M2/91‑68 (MQ=255)
                                                                                                                                       ttttCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaac                                                                    >  2:298097‑M2/1‑24 (MQ=255)
                                                                                                                                        tttCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaacgaattaatttttctcggaaaaacaaatggctatagcacttgtgactggtggcagtcgcg         >  1:208185‑M2/1‑23 (MQ=255)
                                                                                                                                              ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCGTAACCCCCTCCCCAAAGGGGCGAGGGGACTGTCCGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAg   >  2:217013/1‑149 (MQ=255)
                                                                                                                                               cTCGCCTTTTCAGCGAGAGTGCCGGTGAGGGGGTAAATATTCGCGCCAGGGCCGGCCTGTTCCCCTCACCCTAACCATCTCCCCAAAGGGGCGAGGGGACTGTATGAGCAATTTTGTACGTTGTCATCTGACTAAAAAGGCGCCGAAgc  <  1:69115/149‑1 (MQ=37)
                                                                                                                                                 |                                                                                                                                                  
TGCAGTTGCCCGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGC  >  NC_000913_3_pae_tpiA/3045848‑3046139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.