New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3045993	29 (1.170)	4 (0.170)	4/274	2.8	12.9%	intergenic (‑92/+175)	ygfF/gcvP	putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
	?	NC_000913_3_pae_tpiA	= 3046006	26 (1.090)					intergenic (‑105/+162)	ygfF/gcvP	putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Rejected: Frequency below/above cutoff threshold.

CGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGC  >  NC_000913_3_pae_tpiA/3045858‑3046139                                                                                                                                                     |                                                                                                                                      cgccgcaTGCCGCGACGGCCACCAGTCACAAGTGCTATAGCCATTTGTTTCTCTTCTCATAATTACTTTGTTGATGTAGCTTGGAGAGTGCTAACAAACTGGCTGTTTCTTCTATTTTGGTACGCTTTTCCCCCTCGCCCTTTCAGGga                                                                                                                                       <  2:335/143‑1 (MQ=255)         gCCCCGATTGCCACCAGTAACAGGTGCTATAGCCATTTGTTTTTACGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCg                                                                                                                               <  2:122864/149‑1 (MQ=255)           cgcgACTGCCACCGGGCAGAAGTGGTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCggg                                                                                                                             <  2:10552/149‑1 (MQ=255)            gcgACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGCCGTATCTTTGGGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCGCGCCCTTTCCGGGCGGGGCCAgggg                                                                                                                            >  1:139010/1‑149 (MQ=255)                                     ataGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGc                                                                                                   >  2:148252/1‑149 (MQ=255)                                         ccATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGc                                                                                               >  1:180908/1‑149 (MQ=255)                                            tttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTg                                                                                            >  2:330196/1‑149 (MQ=255)                                                tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTcc                                                                                        >  2:264671/1‑149 (MQ=255)                                                tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCATCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTcc                                                                                        >  2:373729/1‑149 (MQ=255)                                                   ttCTGAGAAAAATTAATTCTTTGACGTATCTTTGAGATTGCTAACAAACTGGCTAAATCTTCAATGTTGGCACGCTTTTCCCCCTCGACTTTTCAGGGAGATGGCCGTGGTTAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCt                                                                                     <  2:114242/149‑1 (MQ=255)                                                      cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAc                                                                                  >  1:26846/1‑149 (MQ=255)                                                       gagaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGGGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                 >  1:53347/1‑149 (MQ=255)                                                                                cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                        <  1:148252/149‑1 (MQ=255)                                                                                cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGACGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTGTCCCCAAAGGGGCGAg                                                        <  1:367309/149‑1 (MQ=255)                                                                                  ttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                      <  1:321461/149‑1 (MQ=255)                                                                                  ttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                      >  2:214794/1‑149 (MQ=255)                                                                                      gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACt                                                  >  2:184238/1‑149 (MQ=255)                                                                                       aTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGAGGAGGGGAGTg                                                 >  1:142881/1‑149 (MQ=255)                                                                                          gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                              <  1:214794/149‑1 (MQ=255)                                                                                           cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACTCTAACCTTCTCCCCAAAGGGGCGAGGGGACTGTCTg                                             >  1:146300/1‑149 (MQ=255)                                                                                                 aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaacgaattaatttttctcggaaaaacaaatgg                                       <  2:208185‑M2/149‑97 (MQ=255)                                                                                                 aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                         <  1:112446/99‑1 (MQ=255)                                                                                                 aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                         >  2:112446/1‑99 (MQ=255)                                                                                                       cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTa                                 >  1:350296/1‑149 (MQ=255)                                                                                                                  aTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                            >  2:269675/1‑127 (MQ=255)                                                                                                                  aTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                            <  1:269675/127‑1 (MQ=255)                                                                                                                              ttttCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaac                                                                    <  1:298097‑M2/91‑68 (MQ=255)                                                                                                                              ttttCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaac                                                                    >  2:298097‑M2/1‑24 (MQ=255)                                                                                                                               tttCCCCCTCGCCCTTTCAGGGAgagggggaaaagcgtgccaacattgaagattgagccagtttgttagcaatctcaaagatacgtcaacgaattaatttttctcggaaaaacaaatggctatagcacttgtgactggtggcagtcgcg         >  1:208185‑M2/1‑23 (MQ=255)                                                                                                                                     ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCGTAACCCCCTCCCCAAAGGGGCGAGGGGACTGTCCGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAg   >  2:217013/1‑149 (MQ=255)                                                                                                                                      cTCGCCTTTTCAGCGAGAGTGCCGGTGAGGGGGTAAATATTCGCGCCAGGGCCGGCCTGTTCCCCTCACCCTAACCATCTCCCCAAAGGGGCGAGGGGACTGTATGAGCAATTTTGTACGTTGTCATCTGACTAAAAAGGCGCCGAAgc  <  1:69115/149‑1 (MQ=37)                                                                                                                                                     |                                                                                                                                      CGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGC  >  NC_000913_3_pae_tpiA/3045858‑3046139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.