New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3437896 =	28 (1.130)	3 (0.130)	3/260	3.0	9.9%	intergenic (+2/‑128)	trkA/mscL	NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
	?	NC_000913_3_pae_tpiA	3437924 =	29 (1.280)					intergenic (+30/‑100)	trkA/mscL	NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGATGCGCGGGAACGT  >  NC_000913_3_pae_tpiA/3437776‑3438070                                                                                                                                                     |                                                                                                                                                   tgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTtattat                                                                                                                                                    >  2:78470/1‑149 (MQ=255) tgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTtattat                                                                                                                                                    >  2:220568/1‑149 (MQ=255)     tGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGATTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTcc                                                                                                                                                <  1:174850/149‑1 (MQ=255)        cAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAACACGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCtt                                                                                                                                             >  1:353057/1‑149 (MQ=255)          aTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCGCTTTg                                                                                                                                           >  2:252214/1‑149 (MQ=255)                     cgcATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCCTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGa                                                                                                                                <  2:229355/149‑1 (MQ=255)                              cacggCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                                       <  1:120650/146‑1 (MQ=255)                              cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGTCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                                       <  2:227366/149‑1 (MQ=255)                               aaGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAGTGCGGCTGATAACGCCTTATTTTTTCCCTTTGGTTAGCTAGGatgaagtttat                                                                                                                      >  1:195434/1‑143 (MQ=255)                                    gATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAACGCGTCTAATAACCCCTTATTATTTCCCTTTGATTATACACGATTAATTAAattcctt                                                                                                                 >  2:129642/1‑149 (MQ=255)                                       cACGTAATTATGTTCCTCACAGATAAATAGTTAAATTCTGTTAACGAAAGGCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCt                                                                                                              <  2:195434/149‑1 (MQ=255)                                        aCGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGCCGTCGAAAGACTCTTCCTGCCAAGCCCTTTCTTCTTGTAATTAATAAGTCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGCTTAATTAAATTCATTCCTg                                                                                                             >  1:190308/1‑149 (MQ=255)                                          gTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGc                                                                                                           >  2:254097/1‑149 (MQ=255)                                                   ttCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGGCCTTTCTTCTTGTAATTAATAAGGAGTCTAATGACGCCTTATTATTTCCATTTGATTATCAAGGATTAATTAAATTCATTCCTGCCAGGGAAATg                                                                                                  >  2:401557/1‑149 (MQ=255)                                                       tCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCtt                                                                                              <  1:283047/149‑1 (MQ=255)                                                         acaGATAAAAGGTTTATTCCCGACGTCGAAAGACTATTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCATTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                                            <  2:149112/149‑1 (MQ=255)                                                              tAAAAAGTTTATTAACGACGTTGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAttt                                                                                       <  2:415829/149‑1 (MQ=255)                                                                aaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                                     <  1:16769/149‑1 (MQ=255)                                                                    gTTTATTACAGATGGTGAAAGACTCTTCCAGTGAATCACTTTCTTCTTGGAATTAACAAGGCGTTTAATGATGCCTTATTACTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGa                                                                                 <  2:99374/149‑1 (MQ=255)                                                                            ccGACGTCGAAAGACTCTTCCAGCCAAACCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGCCAGGAAAATGGCTTAACATTTGTTATACTTagcgt                                                                         >  2:43191/1‑145 (MQ=255)                                                                                         aCTCTTCTAGCCAAGTTCTTTCTTCTTGTAATTAATAAGGCGTCTCATGCATCCCAGTTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                                            <  1:395644/149‑1 (MQ=255)                                                                                                     aaGCCCTTTCTTCTTGTAATTAATAAGGCGTCGAAAGACGCCATATTATTTCCCTTTGATTATCAAGGATTAATTAAAATCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCGTAGGGAGAAAAACa                                                >  2:399045/1‑149 (MQ=255)                                                                                                     aaGCCCTTTCTGCTTGTAATTAATAAGGCGTCTGATGACGCCGTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                                <  1:78470/149‑1 (MQ=255)                                                                                                         ccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAg                                            <  1:254097/149‑1 (MQ=255)                                                                                                           tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATACAATGGGCa                                          >  1:347883/1‑149 (MQ=255)                                                                                                           tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGAATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAAAATGAGCa                                          >  2:279505/1‑149 (MQ=255)                                                                                                                   tGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaaa                                  <  1:363051/149‑1 (MQ=255)                                                                                                                               ggCTTCTAATGACGGCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGaa                      <  1:274691/149‑1 (MQ=255)                                                                                                                                    cTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGCGCATTATTAAAGAATTTCGCGAATTTGc                 >  2:184664/1‑149 (MQ=255)                                                                                                                                            gCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGATGcgcg         <  1:220568/149‑1 (MQ=255)                                                                                                                                                   tattTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTGGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGATGCGCGGGAACgt  >  1:247542/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGATGCGCGGGAACGT  >  NC_000913_3_pae_tpiA/3437776‑3438070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.