New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 3600976 =35 (0.960)3 (0.090) 3/256 4.9 8.3% intergenic (‑193/+52) rpoH/ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
?NC_000913_3_pae_tpiA 3601013 = 34 (1.020)intergenic (‑230/+15) rpoH/ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGTGCCAGAGCTTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGC  >  NC_000913_3_pae_tpiA/3600833‑3601119
                                                                                                                                               |                                                                                                                                               
tgtgCCAGAGCTTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCttt                                                                                                                                            <  2:295821/149‑1 (MQ=255)
    ccAGAGCTTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAGATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCt                                                                                                                                        >  1:570006/1‑149 (MQ=255)
     cAGAGCTTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTg                                                                                                                                       >  1:514524/1‑149 (MQ=255)
         gCTTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTAc                                                                                                                                   <  2:494032/149‑1 (MQ=255)
                                ttCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGt                                                                                                            >  1:32238/1‑149 (MQ=255)
                                    cTGTTTTATCAGACAGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGCGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATaa                                                                                                        >  2:555842/1‑149 (MQ=255)
                                      gTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAaca                                                                                                      >  2:45714/1‑149 (MQ=255)
                                                aCCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttatt                                                                                            <  1:412427/149‑1 (MQ=255)
                                                        tttATCCACAATTTTAATGCAAGCTTTTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGTAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                    <  2:520133/149‑1 (MQ=255)
                                                        tttAACCACAAGTTAAATGCAAGCTTGTGAATAAATTAAGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCGGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                    <  2:251249/149‑1 (MQ=255)
                                                              cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGAAATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAg                                                                              >  1:332480/1‑149 (MQ=255)
                                                                    ttCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCCTTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                        >  1:443716/1‑149 (MQ=255)
                                                                       aaaacAAGCTTGTGAATAAATGACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaaa                                                                     <  2:160265/145‑1 (MQ=255)
                                                                       aaTGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaaa                                                                     >  1:418442/1‑149 (MQ=255)
                                                                       aaTGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaaa                                                                     >  1:418434/1‑149 (MQ=255)
                                                                           cAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgtt                                                                 <  2:403842/149‑1 (MQ=255)
                                                                            aaGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttg                                                                <  2:514524/149‑1 (MQ=255)
                                                                               cTTGTGAATAAATTACGCACAAACGGGGACATAGAGGTGAAATACCGGGAAGAGACAACGGGGTATCTTTCCCTGCTACGGAACCCATTACAGGCAGAGAGGACAACACACTGTTATTATTCAGGCGTAAAGTGGCGTAACTGTTGTAc                                                             >  2:218397/1‑149 (MQ=255)
                                                                                 tgtgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCg                                                           <  1:207418/149‑1 (MQ=255)
                                                                                  gtgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGt                                                          <  1:491098/149‑1 (MQ=255)
                                                                                     aaTAAATTACGCACAAAATGTGGCATAGAGATGCAATACCGGGAAGAGACAACGGGGTCTCTTTCACTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACCCCTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGAACCGTGGc                                                       >  2:313773/1‑149 (MQ=255)
                                                                                              cGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGc                                              >  2:275873/1‑149 (MQ=255)
                                                                                                             aTAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCCat                               >  1:19035/1‑149 (MQ=255)
                                                                                                               agagATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTCCGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAGGCCACGCTGCCACCCAGCCAatcc                             >  1:67689/1‑148 (MQ=255)
                                                                                                                      aaaTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCa                      <  2:418434/149‑1 (MQ=255)
                                                                                                                      aaaTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCa                      <  2:418442/149‑1 (MQ=255)
                                                                                                                        aTACCGGGAAGAGAAAGCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATa                    <  1:354455/149‑1 (MQ=255)
                                                                                                                                  gagaCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCaa          <  2:570006/149‑1 (MQ=255)
                                                                                                                                   agaCAACGGGGTCTCTTTCCATGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCCTTTATTATTCAGGCGTAAAGTAGCGTAAATGTTGTACCGTGGCAAGCAACGCTGCCACCCACCCAATCATCGAGCATACCAGCAGCaaa         >  2:625491/1‑148 (MQ=255)
                                                                                                                                     aCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTTCCACCCAGCCAATCATCGAGCATCCCAGCAGCAATAg       >  2:236247/1‑149 (MQ=255)
                                                                                                                                      cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGc      <  1:390489/149‑1 (MQ=255)
                                                                                                                                       aaCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa     <  1:482814/149‑1 (MQ=255)
                                                                                                                                          ggggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTCCAGTGGCGTAAATGTTGTACCGTGGCAAGCCCCGCTGCCACCCAGCCAATCATCGGGCATACCAGCAGCAATAGCAGGc  >  1:253133/1‑149 (MQ=255)
                                                                                                                                          ggggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                        <  1:522954/127‑1 (MQ=255)
                                                                                                                                          ggggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                        >  2:522954/1‑127 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TGTGCCAGAGCTTAAGAGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGC  >  NC_000913_3_pae_tpiA/3600833‑3601119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.