New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3600976 =	35 (0.960)	3 (0.090)	3/256	4.9	8.3%	intergenic (‑193/+52)	rpoH/ftsX	RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
	?	NC_000913_3_pae_tpiA	3601013 =	34 (1.020)					intergenic (‑230/+15)	rpoH/ftsX	RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTT  >  NC_000913_3_pae_tpiA/3600865‑3601157                                                                                                                                                     |                                                                                                                                                 ttCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGt                                                                                                                                                  >  1:32238/1‑149 (MQ=255)     cTGTTTTATCAGACAGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGCGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATaa                                                                                                                                              >  2:555842/1‑149 (MQ=255)       gTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAaca                                                                                                                                            >  2:45714/1‑149 (MQ=255)                 aCCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttatt                                                                                                                                  <  1:412427/149‑1 (MQ=255)                         tttATCCACAATTTTAATGCAAGCTTTTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGTAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                                                          <  2:520133/149‑1 (MQ=255)                         tttAACCACAAGTTAAATGCAAGCTTGTGAATAAATTAAGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCGGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGc                                                                                                                          <  2:251249/149‑1 (MQ=255)                               cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGAAATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAg                                                                                                                    >  1:332480/1‑149 (MQ=255)                                     ttCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCCTTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                                                              >  1:443716/1‑149 (MQ=255)                                        aaaacAAGCTTGTGAATAAATGACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaaa                                                                                                           <  2:160265/145‑1 (MQ=255)                                        aaTGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaaa                                                                                                           >  1:418434/1‑149 (MQ=255)                                        aaTGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTaaa                                                                                                           >  1:418442/1‑149 (MQ=255)                                            cAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgtt                                                                                                       <  2:403842/149‑1 (MQ=255)                                             aaGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttg                                                                                                      <  2:514524/149‑1 (MQ=255)                                                cTTGTGAATAAATTACGCACAAACGGGGACATAGAGGTGAAATACCGGGAAGAGACAACGGGGTATCTTTCCCTGCTACGGAACCCATTACAGGCAGAGAGGACAACACACTGTTATTATTCAGGCGTAAAGTGGCGTAACTGTTGTAc                                                                                                   >  2:218397/1‑149 (MQ=255)                                                  tgtgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCg                                                                                                 <  1:207418/149‑1 (MQ=255)                                                   gtgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGt                                                                                                <  1:491098/149‑1 (MQ=255)                                                      aaTAAATTACGCACAAAATGTGGCATAGAGATGCAATACCGGGAAGAGACAACGGGGTCTCTTTCACTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACCCCTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGAACCGTGGc                                                                                             >  2:313773/1‑149 (MQ=255)                                                               cGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGc                                                                                    >  2:275873/1‑149 (MQ=255)                                                                              aTAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCCat                                                                     >  1:19035/1‑149 (MQ=255)                                                                                agagATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTCCGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAGGCCACGCTGCCACCCAGCCAatcc                                                                   >  1:67689/1‑148 (MQ=255)                                                                                       aaaTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCa                                                            <  2:418442/149‑1 (MQ=255)                                                                                       aaaTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCa                                                            <  2:418434/149‑1 (MQ=255)                                                                                         aTACCGGGAAGAGAAAGCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATa                                                          <  1:354455/149‑1 (MQ=255)                                                                                                   gagaCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCaa                                                <  2:570006/149‑1 (MQ=255)                                                                                                    agaCAACGGGGTCTCTTTCCATGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCCTTTATTATTCAGGCGTAAAGTAGCGTAAATGTTGTACCGTGGCAAGCAACGCTGCCACCCACCCAATCATCGAGCATACCAGCAGCaaa                                               >  2:625491/1‑148 (MQ=255)                                                                                                      aCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTTCCACCCAGCCAATCATCGAGCATCCCAGCAGCAATAg                                             >  2:236247/1‑149 (MQ=255)                                                                                                       cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGc                                            <  1:390489/149‑1 (MQ=255)                                                                                                        aaCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                                           <  1:482814/149‑1 (MQ=255)                                                                                                           ggggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTCCAGTGGCGTAAATGTTGTACCGTGGCAAGCCCCGCTGCCACCCAGCCAATCATCGGGCATACCAGCAGCAATAGCAGGc                                        >  1:253133/1‑149 (MQ=255)                                                                                                           ggggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                              <  1:522954/127‑1 (MQ=255)                                                                                                           ggggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                              >  2:522954/1‑127 (MQ=255)                                                                                                                     tCCCTGTTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCGGCAGCAATAGCAGGCATTCATCGaa                              <  1:579597/149‑1 (MQ=255)                                                                                                                                 aaCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCAtt                  <  1:223603/149‑1 (MQ=255)                                                                                                                                       ttGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATAGAGCATACCAGCAGAAATAGCAGGCATTCATCAAATGATAACCCATTAattgc            >  2:230043/1‑146 (MQ=255)                                                                                                                                           aGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAAc        <  1:45714/149‑1 (MQ=255)                                                                                                                                              gAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTc     <  2:523178/149‑1 (MQ=255)                                                                                                                                                 agagTATAACACGCTTTTATTATGCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGtt  <  1:174444/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTT  >  NC_000913_3_pae_tpiA/3600865‑3601157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.