New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 1477604 =27 (0.740)4 (0.120) 4/264 4.5 13.2% intergenic (+154/‑17) ydbD/ynbA PF10971 family putative periplasmic methylglyoxal resistance protein/inner membrane protein
?NC_000913_3_pae_tpiA 1477623 = 27 (0.790)coding (3/606 nt) ynbA inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATCTGATTAATTGGGGATAATCATTCCTGACAGTGAGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGG  >  NC_000913_3_pae_tpiA/1477475‑1477768
                                                                                                                                                    |                                                                                                                                                 
aTCTGATTAATTGGGGATAATCATTCCTGACAGTGAGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATg                                                                                                                                                   >  1:64372/1‑149 (MQ=255)
 tCTGATTAATTGGGGATAATCATTCCTGACAGTGAGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGa                                                                                                                                                  >  1:360131/1‑149 (MQ=255)
   tGATTAATTGGGGATAATCATTCCTCTCAGTGAGACCCTCTTCTATAAAAATCTTCTGAATTTTTAATGAAACGCCCTGGTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACg                                                                                                                                                <  1:623227/149‑1 (MQ=255)
    gATTAATTGGGGATAATCATTCCTGACAGTGAGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGAATTACGATATATCCGTCAGACACCTCTAGTCAACTTCACGCACACCGAATTATCAAGGATTGATAATGACGc                                                                                                                                               >  1:147611/1‑149 (MQ=255)
             gggATAATCATTCCTGACAGTGAGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGa                                                                                                                                      >  1:582032/1‑149 (MQ=255)
                               aGTGAGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGAAAAAACCGCTCTTTCAGt                                                                                                                    >  1:124139/1‑149 (MQ=255)
                                   aGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATCACGCCCTACCAGCTAAAACCACACTTTCAGCCGCt                                                                                                                >  2:147974/1‑149 (MQ=255)
                                    gTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTg                                                                                                               <  2:135992/149‑1 (MQ=255)
                                     tCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCGAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGt                                                                                                              >  2:577839/1‑149 (MQ=255)
                                        ccAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTaa                                                                                                           <  1:322617/149‑1 (MQ=255)
                                         acaTACCTGGATATATTCTTAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAg                                                                                                          <  1:269987/147‑1 (MQ=255)
                                                         tCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTgg                                                                                          <  2:131954/149‑1 (MQ=255)
                                                           tGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAAGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGAATTGATAATGACGCTCTACCAGATAAAACAGCTCTTTCAGTCGCTGTTAAAGACGACGATGTTTTGGCt                                                                                        >  1:293914/1‑149 (MQ=255)
                                                              aTTTTTTATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGTATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTta                                                                                     <  1:10015/149‑1 (MQ=255)
                                                              aTTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTta                                                                                     >  1:399158/1‑149 (MQ=255)
                                                              aTTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTta                                                                                     <  1:336957/149‑1 (MQ=255)
                                                                                       atagCTCCGTTAGGGAAATTGATGCACGAAAACGCAAACCGAATTATCAAGGATTGAGAATGACGCTTTACCAGATAAAACCGCTCTTACAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAatcac                                                            <  2:530082/149‑1 (MQ=255)
                                                                                          tctcCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAatcacatc                                                         >  1:420383/1‑149 (MQ=255)
                                                                                            tcCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAatcacatcac                                                       >  1:636559/1‑149 (MQ=255)
                                                                                            tcCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGACGACGATGTTTTGGCTTTATAAGCACAACGTTACAGCAAatcacatcac                                                       >  2:16615/1‑149 (MQ=255)
                                                                                              cGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATACCACCACATCGCAAAATGAACCAGCGCCAGCAGGTGCTGTTAAAGCGGACAGCGCGTTTGCGTTATTAGCACCACATTACAGCGAATCACATCActc                                                     >  1:628557/1‑149 (MQ=255)
                                                                                                  aGGGGAATTGATGCACCATATCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGAGAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGCAGCTCTTTTGGCTTTATAAGCACCACGTTACAGCTAATCACATCACTCTtgc                                                 <  1:316757/149‑1 (MQ=255)
                                                                                                    gggAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTtgctg                                               >  1:503294/1‑149 (MQ=255)
                                                                                                    gggAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATAACTCTtcctg                                               >  1:304912/1‑149 (MQ=255)
                                                                                                                               tcaTTATCAATCCTTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTtg                                                  <  1:296813/117‑1 (MQ=255)
                                                                                                                                     tCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGt              <  2:64372/149‑1 (MQ=255)
                                                                                                                                      cAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGtt             <  1:16615/149‑1 (MQ=255)
                                                                                                                                       aaGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTg            >  2:370527/1‑149 (MQ=255)
                                                                                                                                                 aaTGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTggcgg  <  2:527759/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATCTGATTAATTGGGGATAATCATTCCTGACAGTGAGTCCCCAATACCTTGATATATTCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGG  >  NC_000913_3_pae_tpiA/1477475‑1477768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.